Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,436,697	G→A	E60E (GAG→GAA)	trkA →	NAD‑binding component of Trk potassium transporters

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,436,697	0	G	A	100.0%	196.7 / NA	57	E60E (GAG→GAA)	trkA	NAD‑binding component of Trk potassium transporters
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/34);  total (23/34)

CGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCG  >  NC_000913/3436451‑3436938                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   cGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGATATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTTGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAg                                                                                                                                                                                                                                                <  1:271627/251‑1 (MQ=255)      agGGCGACGGCTTCTTTTACTCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACAGTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTTAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTTGCATCCACGCGTATTGCGGGAAGCAGGTG‑cc                                                                                                                                                                                                                                          <  1:172114/251‑1 (MQ=255)              ggCTTCTTTTACGCTATGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATACACGCGTATTGCGGGAAGCAGGTG‑Ccggtgacgc                                                                                                                                                                                                                                  <  1:125457/251‑8 (MQ=255)                cttcttTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGCTGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGAGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCCAGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCg                                                                                                                                                                                                                                >  2:202584/1‑251 (MQ=255)                  tcttTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGa                                                                                                                                                                                                                               <  1:208480/250‑1 (MQ=255)                     tttACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGat                                                                                                                                                                                                                              <  1:336435/248‑1 (MQ=255)                      ttACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGTTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCGCATCCTCGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATg                                                                                                                                                                                                                           <  1:22857/250‑1 (MQ=255)                      ttACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCGTACAGGATAAATTGGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTT‑CCGACGACGCCGATATg                                                                                                                                                                                                                           <  2:440393/250‑1 (MQ=255)                             aatataagcAAAAAGTGTAGAGATAACGGGGCGCGACTGATGAAAATGTGCATGCTGGGGTCCGGCCAGGTTGGAGGCTCACTGGCGGAAAACCTGGTTGGCGAGAACAACGATAGTACTGTTGTCGATCACAACGGTGAGCGTCTGCGGACCTTACATGATTAATTTGAACGGCGGGTGGTGCAGGGGCATTGCTCTCAGCTACGCGTCTTGCGGTAAGCAGGTG‑CCGGCGACGCAGATATGATGGTTg                                                                                                                                                                                                                    <  2:24964/242‑1 (MQ=255)                             aagctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGtt                                                                                                                                                                                                                     <  2:226558/249‑1 (MQ=255)                                   aacAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAAc                                                                                                                                                                                                             <  1:173460/249‑1 (MQ=255)                                          aGTGATGAGATAACGGGTCGCGACGGATGAAAAGTATCATTCTGGGGTCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGAGGACCTGACAGGATAAATTTGACCTGCGGTTCGTGCAGGGGCATGGATCTCATCCACGCGTATTGCGGGAAGCAGGGG‑TCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCa                                                                                                                                                                                                      <  1:186899/251‑1 (MQ=255)                                              gtgaGATAACTGGTCGCGTCTGATGACAATTATCATTCTGGGTGTCGGTCATGTTGGCGGCACTCTGTTGGAAATCCTTGTTGTCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCTTCTTCGGACCTTACAGGATAAATTTGATCTGCGGGTCGTGCAGGGGCATGGCTCTCTTCCGCGCGTATTGCTGGAAGCAGGTG‑CCGACGACGCCGCTATGCTGGGTGCTGTAACCAGTTCacttc                                                                                                                                                                                                  <  2:233367/250‑5 (MQ=255)                                                    tAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAAcc                                                                                                                                                                                             >  1:270147/1‑250 (MQ=255)                                                          gTCGCGACTGATGACAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCCGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGCG‑CCGACGACGCCGATATGCTGGTTGCTGTAAACAGTTAAGATGAAACCAAAATgg                                                                                                                                                                                      >  2:70295/1‑251 (MQ=255)                                                              cgACTGATGAAAATGATCATTCTGGGTGACGGCCAGGTGGGCGGCTCACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTGCTGTTGTCGATACCAACGGTGAGCGTCTGCGTACCTTACAGGATAAAGTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGTGAAGCAGGTG‑CCTACGACGTCGTTATGCTGGTTTCTGTAACCAGTTCAGATGAAACCAATAGGGTtgc                                                                                                                                                                                  <  2:270147/251‑1 (MQ=255)                                                               gACTGATGGAAAATATCATTCTGGGTGCCGGCCAGGTTGGCGGCACCCTGGCGGAACACCTGGTTGGCGAGAACAACGATATTAATGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATACATTTGACCTGCGGTTCGTGCTGGGGCATGGCTCTAATCCCCGCGTATTGCGGGAAGCAGGTGGCCG‑CGCCGCCGATAGGCTGGCTGCTGTACCCAGTTCAGATGAAACCAATATGGTtgcc                                                                                                                                                                                 >  2:201928/1‑251 (MQ=255)                                                                    atgaAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcctgcc                                                                                                                                                                             >  1:76284/1‑250 (MQ=255)                                                                          aTTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGc                                                                                                                                                                      >  1:267269/1‑251 (MQ=255)                                                                            tATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGcc                                                                                                                                                                     <  2:7989/250‑1 (MQ=255)                                                                               cATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTAc                                                                                                                                                                  <  1:240271/250‑1 (MQ=255)                                                                                         gCCGGCTAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCa                                                                                                                                                        >  1:119542/1‑250 (MQ=255)                                                                                                aGGTTGGCGGCACACTGGCGGAAATCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGGCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCt                                                                                                                                                <  2:76284/251‑1 (MQ=255)                                                                                                aGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATTCCAACGGTGAGGGTCTGCGGACCTTACAGGATAAATTTGGCCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCTATATGCTGGTTGCTGTAACCAGTTCAGATTAAACCAATAGGGTGGCTTGCCAGGGAGCCTACTCACTTTTCAACACCCCt                                                                                                                                                <  2:194354/251‑1 (MQ=255)                                                                                                                ggCGGAAAACCTGGTTGGCGCGAACAACGATATTACTGCTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGCCCTGCGGGTCGTGCAGGGGCATGGCTCTCATCAACGCGTATTGCGGCAAGCAGGTG‑CCGACGACGCCGATACGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGt                                                                                                                                 >  2:191022/1‑250 (MQ=255)                                                                                                                 gCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCATATGAAACCAATATGGTTGCCTGCCAGGTAGCCGACTCACTTTTCAACACCCCTAATCGCATCGCTCGTAt                                                                                                                               <  1:394138/251‑1 (MQ=255)                                                                                                                   ggAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATcc                                                                                                                             >  1:296213/1‑251 (MQ=255)                                                                                                                       aaCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCCTGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGCCGACGCCGATATGCTGGTTGCTGTAACCAGTTCCGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTAATCACTTTTCAACACCCCTAATAGCCTCGCTCGTATCCGCTc                                                                                                                         >  2:397088/1‑251 (MQ=255)                                                                                                                           tGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCACCGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGCGCAGGGACATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGCCGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCACTAATCGCATCGCTCGTATCCGCTCAcc                                                                                                                      >  1:180423/1‑250 (MQ=255)                                                                                                                           gggTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCa                                                                                                                     <  2:327683/250‑1 (MQ=255)                                                                                                                                                 tACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGAt                                                                                                <  2:324816/250‑1 (MQ=255)                                                                                                                                                          cGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCCCTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATttc                                                                                      >  2:180262/1‑251 (MQ=255)                                                                                                                                                          cGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTAttt                                                                                       <  1:202584/250‑1 (MQ=255)                                                                                                                                                            aTACCAACGGTGAGCGTCTGCGGACCGTACAGGATAAAGTTGACCGGCGGGTCGTGCAGGGGCATGGCTATCATCCACGCGTATTGCGTGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAAGCGCATTGCTCGTATCCGCGCACCAGACTACGTGCGCGATGCCGATAAGCTATttcat                                                                                    <  2:94077/251‑1 (MQ=255)                                                                                                                                                              aCCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTACGGGAAGCAGGTG‑CCGCCGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGAt                                                                                                >  1:205212/1‑237 (MQ=255)                                                                                                                                                              aCCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCGGCGGGTCGTGCAGGGGCATGGCTCTCATCTACGTGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGGTCAGATGAAACCAATAGGGTTGCCTGTCAGGTAGCCTACGCGCTTTGCAACAACCCTAATCGCATCGCTGGTATCCGCTCACCAGACTACGTGCGCGATGCCGAt                                                                                                <  2:205212/237‑1 (MQ=255)                                                                                                                                                               ccAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTT‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAAGATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATttcattca                                                                                 <  1:367109/251‑1 (MQ=255)                                                                                                                                                                 aaCGGTGAGCGTCTGCGGACCTTACAGGATAAATGTGACCGGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGa                                                                               <  2:267269/251‑1 (MQ=255)                                                                                                                                                                     gTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCt                                                                           >  1:220130/1‑251 (MQ=255)                                                                                                                                                                        aGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGAt                                                                                                <  1:307808/227‑1 (MQ=255)                                                                                                                                                                        aGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGAt                                                                                                >  2:307808/1‑227 (MQ=255)                                                                                                                                                                               gCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTg                                                                 <  1:70295/251‑1 (MQ=255)                                                                                                                                                                                 ggACCTTACGGGATAAATTTGCCCTGCGGGTCGTGCCGGGGCATGGCTCTCATCCACGCGTATTGCGGGCAGCAGGTG‑CCGACGCCGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTCTCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGat                                                               >  2:214109/1‑251 (MQ=255)                                                                                                                                                                                   aCCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGatc                                                              >  1:384888/1‑250 (MQ=255)                                                                                                                                                                                    ccTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATTGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCTCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGatcat                                                            <  1:282791/251‑1 (MQ=255)                                                                                                                                                                                               aaaTTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCTCGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCAc                                                  <  2:334965/250‑1 (MQ=255)                                                                                                                                                                                                        cTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGtt                                        >  1:135333/1‑251 (MQ=255)                                                                                                                                                                                                             ggTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGtt                                    >  1:397213/1‑250 (MQ=255)                                                                                                                                                                                                             ggTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTa                                   <  2:135333/251‑1 (MQ=255)                                                                                                                                                                                                              gTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑ACGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTAt                                  >  1:418305/1‑251 (MQ=255)                                                                                                                                                                                                                cGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCg                                >  1:339170/1‑251 (MQ=255)                                                                                                                                                                                                                   gCAGGGGCATTGCTCTCATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGat                              <  2:118625/250‑1 (MQ=255)                                                                                                                                                                                                                           aTGGCTCTCATCAACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGATCAGTTGGTTATCGATAATATTTAc                     >  2:360673/1‑251 (MQ=255)                                                                                                                                                                                                                                  tcATCCACGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTg               >  1:90807/1‑250 (MQ=255)                                                                                                                                                                                                                                        aCGCGTATTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGt        <  1:320954/251‑1 (MQ=255)                                                                                                                                                                                                                                              aTTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTTCTGTAACCAGTTCAGATGAACCCAATATGGTTGCCTGCCAGGTAGCCTACTCACGTTTCAACTCCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTAGCGATAATATTTACCGACTGAGTGAGTATCCCg  <  2:205854/251‑1 (MQ=255)                                                                                                                                                                                                                                              aTTGCGGGAAGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCg  <  2:90807/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   CGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTG‑CCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCG  >  NC_000913/3436451‑3436938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑