Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,827	C→T	L152F (CTT→TTT)	evgS →	sensor histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,827	0	C	T	100.0%	71.4 / NA	23	L152F (CTT→TTT)	evgS	sensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/14);  total (9/14)

GTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAATAT  >  NC_000913/2484583‑2485072                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gttgCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCAc                                                                                                                                                                                                                                                  <  1:342185/250‑1 (MQ=255)               cGCAGCAACGGGTTCGTTGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTTCCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGAcc                                                                                                                                                                                                                                    <  1:401507/250‑1 (MQ=255)                                                      aTCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGc                                                                                                                                                                                             >  1:175917/1‑250 (MQ=255)                                                                  gagcagtAAATATCATATTAACACTCCGGGAATACGCAGAACTTCAAAAAGCAATGGACGCGCTTGCAGGAGGTGAAGTCGATATAGTGTTATCACATTGAGGTACTTCGCCTCCTCTTAATAATTACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTTGTAACCACCTTTCACGACTCAATGCTACCGCTTTCCCCACCATAACCAGTAAATATTGCTCGGGTAGCacatgtctcccca                                                                                                                                                                                <  2:374349/245‑13 (MQ=255)                                                                               cAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATtc                                                                                                                                                                   <  1:109458/251‑1 (MQ=255)                                                                               cAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATtc                                                                                                                                                                   <  1:309855/251‑1 (MQ=255)                                                                                aaaTTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATtca                                                                                                                                                                  <  2:175917/251‑1 (MQ=255)                                                                                    tAACACTCCGGGAATACGTAGATCATCAAAAAGCAATGGACGAGCGTGCAGAAGGTGAAGGAGATATAGTGTTAGCACAGTTAGTTGCTTCGCCGCCTCTTAATAATGACACTTG‑TGCAACCAAGCCATGGATAATGTCCTTGCCGACGCTGGTAACCACCTTGCACGAATCAATGCGACCGCTTACCTCACCAAAACCAGTAAAGATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATtcatca                                                                                                                                                               <  1:437408/250‑1 (MQ=255)                                                                                       cacTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCatcaatc                                                                                                                                                            >  2:202255/1‑250 (MQ=255)                                                                                              ggAATACGCAGATCATCAATAACAAATGGAAGCGCTTGCAGAAGGTGCCGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATCATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAACTATTGCTCGGGTAGCAAATTACCCCCCAGACGCAGTCATTCATCAATCATTTCCa                                                                                                                                                     >  2:166969/1‑250 (MQ=255)                                                                                               gAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATGCATCAATCATTTCCaaa                                                                                                                                                   <  2:415627/251‑1 (MQ=255)                                                                                                              caAAAAGCAATGGACGCGCCTGCCGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTTCTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTGCCTTTCCGGCGCTGGTAACCACCTGTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGCTAGCAAATTACCCCCCAGACGAGGTATGTCATCAATCATTTCCAAAAGCAACAATTAtct                                                                                                                                     <  2:72258/250‑1 (MQ=255)                                                                                                               aaaaaGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTAATCAATCATTTCCAAAAGCAACAATTATctct                                                                                                                                   >  2:400165/1‑251 (MQ=255)                                                                                                                         ggACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGGAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAtt                                                                                                                         <  1:149958/251‑1 (MQ=255)                                                                                                                                                                         tACTTCGCCCCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAAACAGTAAATAGTGCTCGGGTAGCAAATTACTCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAGTACttt                                                                          <  2:386735/250‑1 (MQ=255)                                                                                                                                                                            ttCGCCGCCTCTTAATAATGACA‑TTGCTGCCACCAAACCATTGCTAATTACATTTCCGGCGCTGGTAACCCCCTTTCAAGACTCCATGCGCCCGCTTAACTCACCAAACCAAGTAAATATTGATCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATAATTTCCAAAAGCAACAATTATCTATTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTg                                                                      >  1:36992/1‑251 (MQ=255)                                                                                                                                                                                                     tgctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGAGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGAAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGt                                               >  2:387675/1‑250 (MQ=255)                                                                                                                                                                                                       ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATg                                            >  1:307437/1‑251 (MQ=255)                                                                                                                                                                                                           caccaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCAACTTTCACGAATCAATGCGACCGCTTCCCTCACCAAAACCAGTAAATATTGCTCGGGAAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGATAGTAACATCATTACCAGCAGTATGAttt                                        >  1:183248/2‑251 (MQ=255)                                                                                                                                                                                                                      tGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtc                             >  1:81106/1‑251 (MQ=255)                                                                                                                                                                                                                       gATAAGTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGGAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATGTCCCCCTATTtca                            <  2:365136/251‑1 (MQ=255)                                                                                                                                                                                                                             tACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcactcac                       <  1:202255/250‑1 (MQ=255)                                                                                                                                                                                                                                                 ccaccTTTCACGACTCAATGCTACCGCTTACCTCACCAAAAACAGTAAATATTGCTCCGGTAGCAAATTACCCACCAGTCGAAGTAATTCATCAATCATTTCCAAAAGCAACAATTAGCTCTTTTACAAATTTCTATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGATATTTCACTCACTCCTTAAATGTAGTGAAatat  <  2:237071/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACA‑TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAATAT  >  NC_000913/2484583‑2485072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑