| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,188,087 | T→C | T110A (ACG→GCG) | yehA ← | putative fimbrial adhesin YehA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,188,087 | 0 | T | C | 95.5% | 60.6 / NA | 22 | T110A (ACG→GCG) | yehA | putative fimbrial adhesin YehA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (11/10); minor base A (1/0); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
ATGTCAGATTAAAGGAATAGACACCTGCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAACAACCAAGTTGTGCTGA > NC_000913/2187856‑2188331 | aTGTCAGATTAAAGGAATAGACACCTGCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTg > 1:42603/1‑251 (MQ=255) aGATTAAAGGAATAGACACCTGCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACt < 1:44287/251‑1 (MQ=255) aaacCTGCATTTTGTGCCACTGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTAATGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGAATCACCGCAACTGAATAACCACTGGTGAAACG‑GGTCTGATTATTTg > 2:334402/3‑250 (MQ=255) gTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAAGTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCAt < 2:112583/250‑1 (MQ=255) aGCTATTTACACATGCATCCCATGACTTGCCACCTAATAATCACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACAGTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCa < 2:23932/251‑1 (MQ=255) aTTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCCCTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAAt > 1:150356/1‑251 (MQ=255) gCATCCCATGACTTGCCACCTAATAAACAAAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGAGAATGTCTCAACCCCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATACACGTTTGCATTACTGGCCGCAGGTGTCAACGCAATTGAATAACTACTGGTGAAACT‑GGTATGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAaattta < 2:302525/250‑1 (MQ=255) tGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATgg > 1:348915/1‑251 (MQ=255) gACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATgg < 2:150356/250‑1 (MQ=255) cTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACCCCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCCCTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACT‑GGTCTGattatt < 2:110901/192‑1 (MQ=255) cTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACT‑GGTCTGattatt > 1:110901/1‑192 (MQ=255) cACCTAATAAAACCAGAAGAAATTGTACTGCGGCACTCCCGCGTGTAGATGATGAAGCACTGGATAATGTAGAAACACCATTAATCATAACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACAGCAACGGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTACATTTAATTTTATGGTATAGtt > 2:388939/1‑250 (MQ=255) cAACACCATTAAGCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGGGTCACCGCAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTa < 1:260280/251‑1 (MQ=255) ttGAACCGCCGCCCCCCGCAGCAACATTCACTTTTGCATTACTGGCCGCAGGTGTCAACGAAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTCAGCATGACGAATCATTAAAATTATGCTCATATgac > 2:82892/2‑249 (MQ=255) gccacccGCAGAAACATTCCCTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTTAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTtgtg < 2:238440/247‑1 (MQ=255) ccccccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAAGTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGGCGCCATTTgtgt < 1:880/251‑1 (MQ=255) aaccatCACCTTTTCATTACCTGCCGCAGGTGGTACCGCAACTGAAAAACCACTTGTGAAACTTGGT‑TTATTAATTTACAACGTTATGTTTTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGGCGCCATTTGTGTTTGAGGAAGTg < 1:332563/246‑1 (MQ=255) ttACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGaac > 1:191768/1‑251 (MQ=255) ttACTCGCCGCAGGTGTCACCGCAACTGAATAACCACTGGAGAAAAT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGaac > 1:358615/1‑251 (MQ=255) tGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAACAAcc > 1:280191/1‑251 (MQ=255) cggTGTCACCGCCACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAACAACCAAGTtgt > 2:386955/2‑251 (MQ=255) tCACCGCAAATGAATAACCAATGGTGAAACT‑GGTCTGCTTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTACCTTTAATTTTATGGCATCGTTATAAAGTCCAACAATAATATCAGCCGGTCTCATAGGGGTCATCGTATTTTTACTTACGCATGACGAATCCTTAAAATTATGCTCATATGTCGCCATTTGTGTTTGTGTAAGTGCACAATCCCCCTGAACAACCAAGTTGTGCTgc > 2:71888/1‑250 (MQ=255) | ATGTCAGATTAAAGGAATAGACACCTGCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACT‑GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAACAACCAAGTTGTGCTGA > NC_000913/2187856‑2188331 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |