Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	313,543	G→A	intergenic (‑301/‑1748)	ykgR ← / → insE1	putative membrane protein YkgR/IS3 element protein InsE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	313,543	0	G	A	100.0%	122.2 / NA	35	intergenic (‑301/‑1748)	ykgR/insE1	putative membrane protein YkgR/IS3 element protein InsE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/21);  total (14/21)

ACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGGAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTTATCCGGAATGG  >  NC_000913/313298‑313786                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    aCGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTg                                                                                                                                                                                                                                                <  2:225617/251‑1 (MQ=255)       ttGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGACGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCt                                                                                                                                                                                                                                          >  1:34534/1‑251 (MQ=255)                           cATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGcc                                                                                                                                                                                                                      <  1:177950/251‑1 (MQ=255)                                                 aTGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTg                                                                                                                                                                                                <  1:333671/251‑1 (MQ=255)                                                              aCTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCAGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTa                                                                                                                                                                                   <  1:105667/251‑1 (MQ=255)                                                                  tCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATg                                                                                                                                                                                 >  1:41616/1‑249 (MQ=255)                                                                        aTTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGCTCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACg                                                                                                                                                                         >  1:158155/1‑251 (MQ=255)                                                                           gCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGc                                                                                                                                                                      <  1:326524/251‑1 (MQ=255)                                                                             aacAACGTCAGTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGGTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTGGTTGGTGACCGGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGAGTACACTGCAGGCACGCCTTATGAGAGAACGTGCCg                                                                                                                                                                    <  2:218725/249‑1 (MQ=255)                                                                               cAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCCGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGcc                                                                                                                                                                     >  2:393020/1‑248 (MQ=255)                                                                               cAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGcc                                                                                                                                                                     <  1:393020/248‑1 (MQ=255)                                                                                 aCGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTAGCTCCTGGGGTGTTAGACAAGATAGCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGt                                                                                                                                                                <  2:302126/251‑1 (MQ=255)                                                                                   gTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCGCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATTCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGa                                                                                                                                                              <  1:351695/251‑1 (MQ=255)                                                                                             tatGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGCTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAAtt                                                                                                                                                    >  1:382431/1‑251 (MQ=255)                                                                                                    ccTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCCGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGa                                                                                                                                              >  1:321213/1‑250 (MQ=255)                                                                                                        aaGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATACTGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGAAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGa                                                                                                                                          >  2:428003/1‑250 (MQ=255)                                                                                                             aGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGGCGTATCTCCTTGGGTGTTATACAAGAGATCGTTGTTGGTGACCTGGGAGAGAAATTGATGTCTATTATGCCGTCAACTATGCCGGAGACAGACTGGATTACACTGCAGTCAGGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTtgt                                                                                                                                    <  2:421080/251‑1 (MQ=255)                                                                                                                 ccGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAgg                                                                                                                                >  1:370520/1‑251 (MQ=255)                                                                                                                            tCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGtt                                                                                                                     >  1:307120/1‑251 (MQ=255)                                                                                                                               gATCTCAAGACTGGCCCAGGCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTa                                                                                                                    <  2:84904/249‑1 (MQ=255)                                                                                                                                    cAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGGTACTCAttg                                                                                                             <  2:355829/251‑1 (MQ=255)                                                                                                                                                       gCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAACCCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACtctc                                                                                          >  1:378547/1‑251 (MQ=255)                                                                                                                                                                     tGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCAc                                                                                                                                                                                         >  1:363356/1‑142 (MQ=255)                                                                                                                                                                             atatCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAAt                                                                    <  1:28592/251‑1 (MQ=255)                                                                                                                                                                                  gCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCAtt                                                               <  1:207966/251‑1 (MQ=255)                                                                                                                                                                                      ctcATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACg                                                           >  2:189207/1‑251 (MQ=255)                                                                                                                                                                                        catcatAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACgg                                                          <  1:191729/250‑1 (MQ=255)                                                                                                                                                                                        catcatAAGGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAAGTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGATAACGTGCCGCAGTGACGTGTTAATTATCTGAAAGAATTTGTGATGCTGTATCGGGTACTCATTGAGTTGATAGTTTTGCTCTCGGGAGAATAATAGATATTTAATCCATTAACg                                                           <  1:46090/249‑1 (MQ=255)                                                                                                                                                                                               aTGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGCGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGCGGCTGTATCGGTTACTCATTGCTTTGATAGTTTTACTCTCGGGGGAATAATAGATATTTAATCCATTAACGGAAAccagc                                                  >  2:192898/1‑251 (MQ=255)                                                                                                                                                                                                                                   tgttgGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACg                                                           <  1:946/206‑1 (MQ=255)                                                                                                                                                                                                                                   tgttgGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACg                                                           >  2:946/1‑206 (MQ=255)                                                                                                                                                                                                                                       ggTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTTAt           <  1:230878/250‑1 (MQ=255)                                                                                                                                                                                                                                            ccTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATGATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCAGTGATTTGATAGTTTTACGCTCGGGAGAATAATAGAGATTTAATCCATTAACGGAATCCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTTATCCGGaa     <  2:382431/251‑1 (MQ=255)                                                                                                                                                                                                                                            ccTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACGATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGATAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGTTGTATCGGTTACTCATTGATTTGATAGTTTTACCCTCGGGAGAATAATAGATATTTAATCCATTTACTGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTGATTCGGaa     <  2:346366/251‑1 (MQ=255)                                                                                                                                                                                                                                               gggAGAGAAATTGAGTGCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGGATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCTTGAATTTGATCCCAGAGTTTATCCGGAATgg  <  1:50258/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGGAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTTATCCGGAATGG  >  NC_000913/313298‑313786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑