Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,715	A→T	S377C (AGT→TGT)	ydiF →	putative acetate‑CoA transferase YdiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,715	0	A	T	100.0%	72.3 / NA	24	S377C (AGT→TGT)	ydiF	putative acetate‑CoA transferase YdiF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/12);  total (12/12)

GTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTACCGGAAATTACTTT  >  NC_000913/1776485‑1776941                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   gTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGAcc                                                                                                                                                                                                                  >  1:22667/1‑249 (MQ=255)       gcgcGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGt                                                                                                                                                                                                          <  1:2145/251‑1 (MQ=255)                  aaTGTTGGCGTCGGTATTGCTGAAGGCATTGGCCTGGTCGCACGAGAAGAAGGTTGCGCTGATGACTTTCTTCTGACGGTAGAAACAGGACCGATTGGCGGTATTACTTCACAGGGGATCACCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGACCCAGTTTGATTTTTATCACGGAGGCGGTCTGGCAGTTTGGTATTTGTGTTTTGCTGAAGTAGACCAGCACGATAACCTCAGCGt                                                                                                                                                                                               >  1:368863/1‑251 (MQ=255)                           ttCGGTATTGCTGCCGCCATTGGCCTGGTCGACCGCGAAGCAGGTTGTGCTGATGCCTTTCTTCTGACGGTAGAAACAGGCCCGCTTGGCGGACTTACTTCACAGGGGATCGCATTTGGCGCGAACGTGAATAGCCGAGCCATTATAGATATGACGTCCCAGTTTGATTATTATCACGGTGGCGGTCTGGATGTTTGTTCTTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGGGCATAAAtt                                                                                                                                                                                      >  2:205655/2‑251 (MQ=255)                                   tGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGt                                                                                                                                                                               >  2:334935/1‑250 (MQ=255)                                                         gCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCg                                                                                                                                                               >  1:8047/1‑244 (MQ=255)                                                         gCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCg                                                                                                                                                               <  2:8047/244‑1 (MQ=255)                                                                 agaagGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGata                                                                                                                                                <  1:216540/251‑1 (MQ=255)                                                                        tgtgCTGATGACTTTATTCTGACGGTCGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGCACGCGAATACCCGTGCCATTCTGGATATTACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCCGCACGGTAACGTCGGCGTGCCTAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGc                                                                                                                                         >  2:189961/1‑251 (MQ=255)                                                                                 gaCTTTATTCGGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGGCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTGATTGATATCAGTGCCACTTCga                                                                                                                                 <  2:53672/250‑1 (MQ=255)                                                                                  aCTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCgaag                                                                                                                               <  1:195399/251‑1 (MQ=255)                                                                                          cTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCAt                                                                                                                       >  1:185329/1‑251 (MQ=255)                                                                                            gACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCAtt                                                                                                                      <  1:5109/250‑1 (MQ=255)                                                                                                     aaCAGGTCCGAATGGCGGAATTACTTCACAGGGGCTCGCCTCTGGCGCGAACGTGAATACCCGTGCCATTCTGGTTATGACGTCCCAGTTTGATTTTTATCACGGCGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGCAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACATCGAAGAAAATCATTTTCTGCGGc                                                                                                             >  2:153759/1‑250 (MQ=255)                                                                                                                  ggCGGACTTACTTCACAGGGGATCGCCTTTGGCGAGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCggg                                                                                               >  2:183076/1‑251 (MQ=255)                                                                                                                      ggaTTACTTCACCGGAGATCGCCTTTGGCGCGAACATGAATACCCGTGCCATACTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGCAGTCGACCAGCACGGTACCGTCGGCGTGCATAAATTCAATGGTAACATCATGGGCCCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCGTTTTCTGCGGCACATTAACTGCGGGCAGt                                                                                           >  2:432080/3‑251 (MQ=255)                                                                                                                                   ggggATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTAGTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTGCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGaaa                                                                              <  1:35460/251‑1 (MQ=255)                                                                                                                                                      aaCGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTGTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGTGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGTGGGCAGTGGAAAAACAGAAATTACCGACGGCAAATTaaa                                                           <  1:262795/251‑1 (MQ=255)                                                                                                                                                                          tCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACggg                                       >  1:325816/1‑251 (MQ=255)                                                                                                                                                                             ggttatGACGTCTCAGTTTGATTTTTATCACGGTGGCGGGTTGGATGTTTGGTATGTGTGTTTTGCTGAAGCCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCAGGGGCACCGGTGGATTTAGTGATATCAGTGCCACTTCGAAGAAAATCATTTTCGGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGAAAAATAAATATCGTCCAGGTAGGTCGGTTga                                    <  2:279847/248‑1 (MQ=255)                                                                                                                                                                                            gTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCggg                     <  2:329706/251‑1 (MQ=255)                                                                                                                                                                                                      tATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTACCgg            <  1:334935/250‑1 (MQ=255)                                                                                                                                                                                                           cGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTACCGGAAAtt       >  2:267449/1‑250 (MQ=255)                                                                                                                                                                                                               ggCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGTGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAAGCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCTTCCAGGAAGGACTGGTGAAGAAATTTATTCGGGAACTACCGGAAATTACttt  <  1:19056/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   GTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTACCGGAAATTACTTT  >  NC_000913/1776485‑1776941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑