Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,711,338	G→A	V171I (GTA→ATA)	rsxE →	SoxR [2Fe‑2S] reducing system protein RsxE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,711,338	0	G	A	100.0%	106.2 / NA	32	V171I (GTA→ATA)	rsxE	SoxR [2Fe‑2S] reducing system protein RsxE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/15);  total (17/15)

GCCTACGCCTTTGGCCTGTATCAATCATTAGGGATTTTTATTCCGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAGTATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCAT  >  NC_000913/1711092‑1711579                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gcctacgcctTTGGCCTGTATCAATCATTAGGGATTTTTATTCTGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAATTTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATAtt                                                                                                                                                                                                                                               <  1:336801/251‑1 (MQ=255)  cctacgcctTTGGCCTGTATCAATCATTAGGGATTTTTATTCCGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATAtt                                                                                                                                                                                                                                               >  1:340186/1‑250 (MQ=255)       gcctTTGGCATGTATCAATCATTAGGGATTTTTATTCCGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGCCGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGGTTGCCGGTGCAGATGCGATGTTAGGTAGCTGGGCAAAAATATTAcgagg                                                                                                                                                                                                                                         >  2:129932/1‑248 (MQ=255)                     tcaatcaTTAGGGATTTTTATTCCGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCcac                                                                                                                                                                                                                            >  1:51994/1‑250 (MQ=255)                                                gATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGAt                                                                                                                                                                                                  <  1:181143/249‑1 (MQ=255)                                                          aaCTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAACTTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTACCCTTTCCTGCTGGCGATGCTGCCACCAgg                                                                                                                                                                                      >  2:337934/1‑251 (MQ=255)                                                                aTCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCCCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCACTGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAggggcctt                                                                                                                                                                                >  1:241583/1‑245 (MQ=255)                                                                 gcgttggtggCCGCGCTGAAGCCGGCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGAAGGCTTTTCAATTGGGAGGGGCGCAACCTGCGCCATGTTCGTGCTGGGTGCACTACGCGAAATTATCGGCAATGGCACATTGTTGGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGGTGAGATTGGCCACAACGACTCCCCTTTACTGCTGGCGATGCTGCCACCAGGTGCAttt                                                                                                                                                                               <  2:440456/243‑1 (MQ=255)                                                                     tgtgGGCCGCGCTGAAGCCTTCGCCGCCTAAAAAGGTCCGGCGCTTGCGGCCCTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCGTTCCTGCTGGCGATGCTGCCCCCTGGTGCATTTAtt                                                                                                                                                                            <  2:330722/250‑1 (MQ=255)                                                                                   aaGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATg                                                                                                                                                             <  1:227137/251‑1 (MQ=255)                                                                                             cgccAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGa                                                                                                                                                    >  1:7718/1‑250 (MQ=255)                                                                                                   aaaaGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGTCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAGAATAcc                                                                                                                                             <  1:112712/251‑1 (MQ=255)                                                                                                           cGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGACATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATACGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATCTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCtgattgat                                                                                                                                     >  1:287377/1‑251 (MQ=255)                                                                                                               gcTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGaaa                                                                                                                                 <  1:20987/251‑1 (MQ=255)                                                                                                                  ttCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAg                                                                                                                                <  2:340186/249‑1 (MQ=255)                                                                                                                   tCGGCAATGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGAGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTACTGCTGGCGATGCTGCCACCAGGTGCTTTTATTGGCCTGGGACTGATGCTGGCAGGGACATTCCTGTTTTATGCAAGAAt                                                                                                                             >  2:158420/1‑251 (MQ=255)                                                                                                                               ggCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCg                                                                                                                 >  1:327145/1‑251 (MQ=255)                                                                                                                                gCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTATTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGt                                                                                                                <  1:302444/251‑1 (MQ=255)                                                                                                                                   tttCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTg                                                                                                               <  1:12329/249‑1 (MQ=255)                                                                                                                                             tATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGACGCAGCTg                                                                                                   >  2:9557/1‑251 (MQ=255)                                                                                                                                                gggCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGATATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCTACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATTAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCa                                                                                                 <  1:149805/250‑1 (MQ=255)                                                                                                                                                      aaCCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTg                                                                                          >  1:70632/1‑251 (MQ=255)                                                                                                                                                       aCCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGc                                                                                         >  2:321309/1‑251 (MQ=255)                                                                                                                                                                aTGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGATGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGCGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGTGACTGCTGCTGGCAGGAAAATACCTGCTTGATTAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCat                                                                                 >  2:344576/1‑249 (MQ=255)                                                                                                                                                                                cACTACGCGAAATTATCGGCAATGGCACATTGTGTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGAGTCCCCTTTCCTGCTGGCGATGCTGCCAGCAGGGGCATTTATTTGCCTGGGACTGATGCTTGCAGGAAAATACCTGATTGATGAAAGGATGAAAAATCGCTGTGCGGATTCAGCTAGAGAACGGGCATTGCCagacttttaaacatggaat                                                                <  2:58114/251‑19 (MQ=255)                                                                                                                                                                                  cTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGAGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCAACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCTAAACGGTGAAACAGGGAATgg                                                              >  1:391644/1‑250 (MQ=255)                                                                                                                                                                                                  gCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCaa                                              <  1:58376/251‑1 (MQ=255)                                                                                                                                                                                                                        tGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGCAAATACCTGATTGATGAAAGAATGAAACAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCCGGAGATCCTCACTCg                        >  2:114887/1‑251 (MQ=255)                                                                                                                                                                                                                           aGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTACCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCt                     <  1:337934/251‑1 (MQ=255)                                                                                                                                                                                                                                              tGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGCTGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGCAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACCGGGAATGTCTGATGAATACAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGGGAGAACAATCCTCAt  >  2:355244/1‑251 (MQ=255)                                                                                                                                                                                                                                                ggCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAg                                                                     >  1:60061/1‑182 (MQ=255)                                                                                                                                                                                                                                                ggCAAAAAGATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCGGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGGGCTGAAGCAGCTTCAGAACGTGCATTGCCAAACGGTGAAACAg                                                                     <  2:60061/182‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GCCTACGCCTTTGGCCTGTATCAATCATTAGGGATTTTTATTCCGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAGTATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCAT  >  NC_000913/1711092‑1711579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑