Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,366,433	G→A	G295D (GGC→GAC)	puuE →	4‑aminobutyrate aminotransferase PuuE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,366,433	0	G	A	100.0%	111.7 / NA	37	G295D (GGC→GAC)	puuE	4‑aminobutyrate aminotransferase PuuE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/17);  total (20/17)

AACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCAC  >  NC_000913/1366189‑1366663                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         aaCGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTa                                                                                                                                                                                                                                    <  1:26836/251‑1 (MQ=255)   cGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACg                                                                                                                                                                                                                                  >  1:418743/1‑251 (MQ=255)                     ggTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGc                                                                                                                                                                                                                  <  1:153480/249‑1 (MQ=255)                           cGCTATTCGCCGCCTGTGTGACGAGCACGGTATTGTGATGATTGCTGATGAAGTTCAAAGCGGCTTTGCGCGTTCTGGTAAGCTGTTTGCCATGGATCATTACTCCTATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCAGGTAACCCGCTGGCGGTgtcgg                                                                                                                                                                                                          <  2:331320/251‑5 (MQ=255)                                   gccgccTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCAGTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGGCGGTAACGCGAATATTATGGACGCGCCCGCGCCGGGCTGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCAc                                                                                                                                                                                                  <  2:46525/251‑1 (MQ=255)                                    ccgccTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCAcg                                                                                                                                                                                                 >  1:394926/1‑251 (MQ=255)                                        cTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCggg                                                                                                                                                                                             >  1:82638/1‑250 (MQ=255)                                         tgtgCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTg                                                                                                                                                                                            >  2:79959/1‑251 (MQ=255)                                              gacgaGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGAGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGACGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCa                                                                                                                                                                                       >  2:50224/1‑250 (MQ=255)                                                          aTTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACaa                                                                                                                                                                          <  1:57695/251‑1 (MQ=255)                                                             gtgATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAg                                                                                                                                                                        <  1:251430/250‑1 (MQ=255)                                                                   atTGCTGATGAAGTGCAAAGCGGCATTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGCCGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCCGCCGCGCACGCGGTG‑CTCAACATTTTCTCCAAAGAATCA‑cc                                                                                                                                                                >  2:39526/1‑250 (MQ=255)                                                                    ttGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAAAATCA‑ctc                                                                                                                                                               >  1:439946/1‑251 (MQ=255)                                                                                            ttGCGCGTACCGGTAAGCTGTGTGCCATGGATCATTACGCCTATAAGCCGGATTTAATTACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCATCTggg                                                                                                                                        <  2:141626/250‑1 (MQ=255)                                                                                                              tGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACg                                                                                                                     >  1:370673/1‑251 (MQ=255)                                                                                                               gTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGGCACCACTGGGCCAGCGTCCCACAAACACGt                                                                                                                    >  2:202024/1‑251 (MQ=255)                                                                                                                   gCCATGTATCATTGCGCCGAGTAGCCGGTTGTAAGGACGATGGCGAAAGGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTATCGCGAAGATGATGGACGCACCCGCGCCGGGCGGGCTTTTCGACACCTACGCCGGTAACCCGCTGGCGGTTGCTGCCGCGCACGCG‑TGTCTCAACATTAGCGGCAAAGAATCA‑CTCTGCGAACTCGCGAATCAACGGGGCCAGCGTCGCAAAAACTCGttgtt                                                                                                                <  2:41221/251‑3 (MQ=255)                                                                                                                      aTGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCATCGCGGCCGGGATGCCGCATTCGGGCGTGGTCGGTAACGCGTATCTTATGGACGCAACCGCGCCGGGAGGGCTTGGCGACACCTACGAAGGGAACCCGCCGGAGGTGGCTGCCGCGAACGCGGGG‑CCCAACCTTATAG‑CAAAGAAACAACCCAGCGAACGCGCGAATCAACTGGGCCACCGtaccaaacaaacgaagactg                                                                                                              >  2:374001/1‑231 (MQ=255)                                                                                                                          aTCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGcc                                                                                                         <  1:184739/251‑1 (MQ=255)                                                                                                                             aTTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCaaa                                                                                                      <  2:91643/251‑1 (MQ=255)                                                                                                                              ttACGCCGATAAGCCGGATTTAATGACGATTGCGAAAAGCCTAGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGATAACGCGAATATTATGGACGAACCTGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGGGGGCGGCGCGCACGACGTG‑CACCACAATATCAACCAAGAATTA‑CTCTGCGAACGCGCGAAACAACTGGGCCAGCGTCTCCAAAACACGATGATTTATGCCaaa                                                                                                      >  2:65939/1‑250 (MQ=255)                                                                                                                              ttACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCCACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCaa                                                                                                       >  1:292881/1‑249 (MQ=255)                                                                                                                                 cgccgcaaaGCCGGCTTTATTGACGATGGCTAAAAGCCTAGAGGGCGGGCTGCCCCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCCCCCGCGCCGGGCGGGCTTGGCGACACCTAAGCCGGAAACCCGCTGGCGGGGGCTGCCGCGCACGCGGGA‑CTCAACATTATCGACAAAGCATCA‑CTCTGCGCACGCGCGAATCAACTGGGTCAGCGTCTCAAAAACCCGTTGATTGATGACaaacaa                                                                                                   >  2:174992/8‑247 (MQ=255)                                                                                                                                              gATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCAt                                                                                     >  1:297584/1‑251 (MQ=255)                                                                                                                                                    aggACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTGTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCTAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTGCCGGCCATtgctgc                                                                               <  2:414679/249‑1 (MQ=255)                                                                                                                                                      gACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCTCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCgg                                                                             <  2:418743/251‑1 (MQ=255)                                                                                                                                                      gACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCgg                                                                             >  1:75422/1‑251 (MQ=255)                                                                                                                                                            ggCGAAAAGCCTCGCGGGCGGGATGCCGCTTTAGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGAGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGCAcgcg                                                                       >  1:28005/1‑251 (MQ=255)                                                                                                                                                                  aaGCCTTGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTgg                                                                 >  1:122873/1‑251 (MQ=255)                                                                                                                                                                        cgggggcgggATGCCGCTGTCGGGCGGGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTTTCAAAAACACGGTGATTGAGGTCAAAGAAAGCGGTCGGGCGATTGCTGCGGTACGCGGCCTGGGGTc                                                             <  2:183864/246‑1 (MQ=255)                                                                                                                                                                                  ttGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCAGGTAACCCGCTGGCGGTTGCTGCCGCGCACGCGGTG‑CTCAACATTATCGCCAAAGACTCA‑CTCTGCGAACGAGAGAATCAACTGGGCCAGCGACCCAAACACCCGTTGATTGATGCCACAGAAAACGTTCCGGCCATTGCTGCGGTACGCGGCCCGTGGTCgatgactccggg                                                 >  2:16563/2‑244 (MQ=255)                                                                                                                                                                                           tCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTa                                         <  1:50224/250‑1 (MQ=255)                                                                                                                                                                                                cGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATc                                   <  1:243292/251‑1 (MQ=255)                                                                                                                                                                                                                        ggACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAgcg            <  1:202024/250‑1 (MQ=255)                                                                                                                                                                                                                             cACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGGGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACGGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGAtt      <  2:394926/251‑1 (MQ=255)                                                                                                                                                                                                                                 cgcgCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGcac  >  1:3385/1‑251 (MQ=255)                                                                                                                                                                                                                                   cgcCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGCATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGcac  >  1:363311/1‑249 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         AACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTG‑CTCAACATTATCGACAAAGAATCA‑CTCTGCGAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCAC  >  NC_000913/1366189‑1366663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑