| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,358,859 | T→C | Y110C (TAT→TGT) | puuP ← | putrescine:H(+) symporter PuuP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,358,859 | 0 | T | C | 95.7% | 63.3 / NA | 23 | Y110C (TAT→TGT) | puuP | putrescine:H(+) symporter PuuP |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (9/13); minor base A (1/0); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
AACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTG > NC_000913/1358624‑1359083 | aaCGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAACCACCACCATGTTGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATTGCGAACAA‑GGTTACCACCCACACCCACGGCGGCACTTCCCGGGAGAGGGCGGGGAGAGAGATTTTCTCCAACAAACC‑GTTAATCAACCGCAAAAAGAGACAAACCAGCAGCGATg > 2:151882/1‑251 (MQ=255) tctcCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAg < 2:400117/249‑1 (MQ=255) cTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGCA‑GGGTACCACCCCCCCCCACGGCGGCACTTCCGGGCAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCcagcag > 1:65500/1‑222 (MQ=255) cTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGTCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GGGAATCGTCGTCAAAATGAGTCAATCcagcag < 2:65500/222‑1 (MQ=255) tGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTTAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCCCTTCCGGGAAGAGGGGGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAAACAATCCCGCAGCGATGGCCAGCCCACCCTAAATCCGACGTg > 1:426815/1‑251 (MQ=255) gAACTACCAGGAAGATAAACACCTCCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGCTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGAGTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCGGACCATAAATCCGACGTGc < 2:170180/251‑1 (MQ=255) gAACTACCAGGAAGATAAACACCACCATGAGTGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCAACGGCAAAAAGAGTCAATCCGGCAGCGATGACCAGCCGACCATAAATCCGACGTg < 1:178853/250‑1 (MQ=255) atgAAGATAAACACCACCATGATGGAGATGTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGTCCTTCAGAGTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACTCCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAAAAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCAGACCATAAATCCGACGTGCGGGTTAAt < 2:254826/249‑1 (MQ=255) gATAAACACCACCCTGATGGAGATTTAAACCAGTACAAACACGGTATTGACGTTAGCGCCCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATAGCGACAAAAGTTTACAACCC‑CACCCAAGGCGACACTTTCGGGGAGAGGGCGGAGAGATAGAATTTTACCAACAA‑ACACTTAAACAACGGCAAACAGAGAACATACAACAGCGAAGAACAGACGACCATAAAtaacgcctgggagttcataaaat > 2:285337/1‑229 (MQ=255) aaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGCATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGACGAA‑GGGTACCACCCACACCCCCGGCGGGACTTCCGGGAAGAGGGCGGAGAGAAAAATTTTCGCCAACAAGAC‑GGTAATCATCGGCAAAAAGAGACAATCCCGCCGAGATGACCAGCCGGCCATAAATCCCACGTGCGGGGTAAACGACttttt > 2:300693/1‑250 (MQ=255) gCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACCGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAAcc < 1:85232/250‑1 (MQ=255) ggTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGGCTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGc < 2:28367/251‑1 (MQ=255) tGACGCTCTTCAGAGTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCCCCCACACCCACGGCGTCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAAGCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTGGCCGTATCTGAt < 2:112047/250‑1 (MQ=255) ctctTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTa < 1:294622/191‑1 (MQ=255) tCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGcac < 1:318099/250‑1 (MQ=255) gaA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAACAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAgg > 1:166665/1‑251 (MQ=255) ccacccacACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCgg > 1:340881/1‑250 (MQ=255) acccacacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATAGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAAc > 1:85990/1‑251 (MQ=255) ccggcggCCCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGCCCAGCCGACAATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGAAAACTGGCGACCCAGTTTGCCTTAGCTTATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCg < 1:33193/250‑1 (MQ=255) ccccTTCCGGGAAGAGGGCGGAGAGATAGATTTCCGCCAACAAGAC‑GTTAATAACCGGCAAAAAGATACAATCCAGCAGCGATGACCAGCCGACCATAAACCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATg < 1:256192/248‑1 (MQ=255) gAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTACTGCCGGAGACa > 2:333756/1‑250 (MQ=255) gatagatTTTCGCAAACAAGAC‑GTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACGGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCa < 1:300693/251‑1 (MQ=255) c‑GTTAATCATCGGAAAAAAGAGACAATACAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGAATCAAATACGGTCATGGGCGTg > 2:172498/1‑248 (MQ=255) | AACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAA‑GGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGAC‑GTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTG > NC_000913/1358624‑1359083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |