Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,356,932	G→A	T60I (ACC→ATC)	sapA ←	putative periplasmic binding protein SapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,356,932	0	G	A	100.0%	103.4 / NA	31	T60I (ACC→ATC)	sapA	putative periplasmic binding protein SapA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/13);  total (18/13)

CCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTG  >  NC_000913/1356686‑1357165                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          ccGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATc                                                                                                                                                                                                                                       <  2:95265/251‑1 (MQ=255)   gTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGACACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCg                                                                                                                                                                                                                                      >  1:89379/1‑250 (MQ=255)          ttATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTa                                                                                                                                                                                                                               >  1:204558/1‑250 (MQ=255)                                 aaaaaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTGCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATggga                                                                                                                                                                                                       <  2:135872/251‑2 (MQ=255)                                         gCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGgtg                                                                                                                                                                                               >  1:52597/1‑251 (MQ=255)                                                   aaaCACCACAGCGTCGGCATTCATGTTACGAGGGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGcc                                                                                                                                                                                     <  2:204558/251‑1 (MQ=255)                                                                    cattcattTTACGAGTGGGAGTAAACCAGTCGGGTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCAGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGAGATAGGTCTAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGAc                                                                                                                                                                     >  1:358879/1‑250 (MQ=255)                                                                                  gTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTcgcg                                                                                                                                                      >  1:232536/1‑251 (MQ=255)                                                                                   tGGGAGTAAACCAGTCGGTTTTTTGAAACGGCACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCgg                                                                                                                                                     >  1:45529/1‑251 (MQ=255)                                                                                          aaaCCAGTCGGTGTTTTGAAACGGAACATCGCGACGCAGGTTGAAGCGATAGGTCGCGTCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGGCGATCATAAAACTGGGCGGCAAGGATAGCGACACTTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTTGCGGATATCa                                                                                                                                               <  2:393580/250‑1 (MQ=255)                                                                                           aaCCAGTCGGTTTTTTGAAACGGAAAAACGCGACGCAGGTGGAAGCGATAGGTCGAGCCGTTATCGAGTACTTCCCAGCTTTCGGCAAGTTCAGGCCTCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAAATGGGCGGACAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGATGTTGACTTGCCCGCTGACGCAATAGACAAACCCGCGGTCGCGGATATCAGc                                                                                                                                             >  2:406563/1‑251 (MQ=255)                                                                                              cAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATg                                                                                                                                          >  1:113508/1‑251 (MQ=255)                                                                                               aGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGc                                                                                                                                         >  1:408673/1‑251 (MQ=255)                                                                                                 tCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCgg                                                                                                                                       >  1:100766/1‑251 (MQ=255)                                                                                                        tttGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACGTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATAGCGACAATTAACCCACTGCGCGCTGTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGgaga                                                                                                                                 <  2:210288/250‑1 (MQ=255)                                                                                                                             gCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGa                                                                                                           <  1:96935/251‑1 (MQ=255)                                                                                                                                             ggTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAg                                                                                           <  2:408673/251‑1 (MQ=255)                                                                                                                                                cgcgCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCa                                                                                         >  1:86473/1‑250 (MQ=255)                                                                                                                                                 gcgcCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAAt                                                                                       <  1:37308/251‑1 (MQ=255)                                                                                                                                                     cGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCAcc                                                                                   >  2:124189/1‑251 (MQ=255)                                                                                                                                                             aGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAgaga                                                                           <  2:96997/251‑1 (MQ=255)                                                                                                                                                                                   gTTCCGACATCAGGCGATAGGTATAGGTCTCGACATCCAGCAGTCGCTCATAAAACTGGGCAGCAAGGATATCGACAATTCACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACACAATAGACAAAACCGCTGACGCGGATATCAGCATGAGGGGGAGATTCAGGCGCGGCGATTGCCTGCCCACTCACAAGTCCAGCAATCACCAAAAGAGAAGATAATACCTGGCGCataat                                                      >  2:88772/1‑250 (MQ=255)                                                                                                                                                                                         gCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTAGATCATAAAACGGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCAGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAgg                                               <  2:146613/251‑1 (MQ=255)                                                                                                                                                                                          cATCAGGCGATAGGCATAGGGAGCGACATACAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGCTAACGGTGTATACTTACCCGCTGAAGCAATAGACAAAACCGCTGTCGCGGGTATCAGCATGCGAGGGAGATTCAGACACGTCGATTGCCTGACAACACACACGTCCAGCAATCGCAAAAAGATAAGATAATAACTGGAGCATACTATTAAg                                                >  2:228554/1‑249 (MQ=255)                                                                                                                                                                                              aGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCCTAATATTAAGGGAttt                                          >  2:414875/1‑251 (MQ=255)                                                                                                                                                                                                gCGATATGTTTAGGGATCGACATCCGGCATTCGATCATAAAAGTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGTGGCGATTGCCTGACGACTCACAATTCCAGCAATCACCAAAAGAGTCGATATTACCTGGAGCATGATATTAAGGGATTTTa                                        <  2:246279/251‑1 (MQ=255)                                                                                                                                                                                                                              tCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAAt          >  1:118226/1‑251 (MQ=255)                                                                                                                                                                                                                                gATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGa        <  1:126319/251‑1 (MQ=255)                                                                                                                                                                                                                                      aaaaCTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGt                                     <  2:108128/216‑1 (MQ=255)                                                                                                                                                                                                                                      aaaaCTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGt                                     >  1:108128/1‑216 (MQ=255)                                                                                                                                                                                                                                      aaaaCTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTg  >  1:162748/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          CCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTG  >  NC_000913/1356686‑1357165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑