Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,322,325	G→A	L208F (CTC→TTC)	trpE ←	anthranilate synthase subunit TrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,322,325	0	G	A	100.0%	73.5 / NA	23	L208F (CTC→TTC)	trpE	anthranilate synthase subunit TrpE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/11);  total (12/11)

GGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTC  >  NC_000913/1322080‑1322536                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                    gggATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACgtt                                                                                                                                                                                                                <  1:32515/251‑1 (MQ=255)       aCTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGttttt                                                                                                                                                                                                          >  1:29470/1‑251 (MQ=255)             tttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTAGCTGCGGCGGCGCGGCTTCCGTCAGTTGCTGCCGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTttcttctt                                                                                                                                                                                                    >  2:1172/1‑251 (MQ=255)              ttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCGGGCGAGCAGTGAAACGTTGTTTttcttcttc                                                                                                                                                                                                   >  1:226554/1‑251 (MQ=255)              ttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTttcttctt                                                                                                                                                                                                    <  1:124546/250‑1 (MQ=255)               tCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTttcttcttc                                                                                                                                                                                                   <  1:71036/250‑1 (MQ=255)                 aGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGTGAGAAACGGCGAGATGGCACCACCCGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGTCACGGAACCCACTGGCAGCGGCGGCTCGGCTTCGGTCAGTTGCTGACTTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCAtt                                                                                                                                                                                                <  1:375054/251‑1 (MQ=255)                                    gCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGcc                                                                                                                                                                             <  1:100538/251‑1 (MQ=255)                                     cGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCTCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCt                                                                                                                                                                            <  1:216412/251‑1 (MQ=255)                                                                        gCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCGATCAcc                                                                                                                                         <  1:114743/251‑1 (MQ=255)                                                                                 ggAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATGCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCg                                                                                                                                  <  2:138332/249‑1 (MQ=255)                                                                                            ccAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCgaga                                                                                                                       <  1:269482/249‑1 (MQ=255)                                                                                                        gCTTTTTGCAAAACACACACTACGACACCGAACTCTTAATCGCTATGATTACATTCACAACGCATATGCAGCAAGGCCACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGATGACGAAGTTCGGTCAGGCGAGCCGTGAAACGTTGTTTTTCTTCTTCATTCGGAGAAAACAGGCTGGCCTGCATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATACAAACAGAAATc                                                                                                         >  2:248334/1‑251 (MQ=255)                                                                                                                     aaCGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTAttt                                                                                            <  1:1172/251‑1 (MQ=255)                                                                                                                          aCTACGCCAACGACCTACTCCTCGCTCTGATTATATTCACAACGCATATTCGGCACAGAACCCACTGGCAGCGGACGCCCAGCTTCGGTCAGTCGCTGCCGCAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGACTACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGGGTTTCAGCGAGGTAAAAACAGAAATCAGGGCAGTTATTTTCCGc                                                                                       >  2:353364/1‑251 (MQ=255)                                                                                                                                      aacacttaaTCGCTCTGATTACATTCACAACGCATATGCGGCACGGACACCACTGGAAGCGGCGGCGCGGCTCCGGTCAGTTGCTGAAGTAATTCGTTCAGGCGAGCAGTGAATCGTTGTTTTTCTTCTTCATTCGGTGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTATGATGGTCAATCACCATCAGCGTTTCCGCGAGATAAACACAGATATAAGGGCAGTTTTTTTCCGCTGTCAGTTGCgg                                                                           >  2:359842/9‑251 (MQ=255)                                                                                                                                           ttCATAGCTCGGATTACATTCACAACGCAAATGCGTCACGGAAACCACTGGCAGCGGCGGCGCGGCTTAGGACAGTTGCTGACGACGTTCGTTCAGGCGAGCAGGGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGGATACGGGGGCTTTTTTTCTGCTGGTCAATCACCATCAGCGTTTCAGCGAGATAAAATCAGAAAGCAGGGAAGGTATTTTCCGCTGACAGTTGCGGTAAAt                                                                      >  2:20570/1‑251 (MQ=255)                                                                                                                                                       aTTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATCCGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGAGGTAAATCTTCAACTCCCg                                                          >  2:277788/1‑251 (MQ=255)                                                                                                                                                                acaACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATAAGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGATGCGGTAACTCTTCAAATCCCGCCACAAGGt                                                 >  2:307514/1‑251 (MQ=255)                                                                                                                                                                                       ccACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCga                            >  1:245861/1‑249 (MQ=255)                                                                                                                                                                                              cAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAACCGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGCATACGGGTGCTTTTTTTCTGATGGTCAATAACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCACATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGCACATgg                   >  2:368768/1‑251 (MQ=255)                                                                                                                                                                                                                tgTCAGTTGTTGGCGTTGTTCGTTCAGGCGAGCAGGGAAACTTTGTTTTTTTTTTTCATGAGGAAAAAACAGGGTGGTCTGAAGAGGGTTTTTTTTTTTCTGATGGTCAACCACCACCAGCTTTTCAGGGAAAAAAAAACAGAAAACGGGGCAGTTATTTTCCGCGGACAGTGGCGGAAAATCTTCAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttccttc  <  2:245861/249‑1 (MQ=255)                                                                                                                                                                                                                                        cAGGCGAGAAGTGAAACGTTGTATTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGACTACGGCTGCTTTTTTTCTGCTGGCCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGCAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTAAAATCCCGCCACAAGGTCATAAGAGAACAGGACGCCGAAGAACATGGCTTCTCGttct        >  2:420579/1‑220 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                    GGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTC  >  NC_000913/1322080‑1322536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑