Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,317,129	G→A	Q32* (CAG→TAG)	trpA ←	tryptophan synthase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,317,129	0	G	A	100.0%	115.0 / NA	34	Q32* (CAG→TAG)	trpA	tryptophan synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/15);  total (19/15)

ACTCATCAATGCCTTTGTTAAACACCAGATTGGCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGC  >  NC_000913/1316883‑1317374                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      aCTCATCAATGCCTTTGTTAAACACCAGATTGGCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTc                                                                                                                                                                                                                                                    >  1:103565/1‑250 (MQ=255)           gccttggtgaacccccAGATTTGCATACATCAACTGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACTTTGTGCCGGAGTCACACCGGCCGCAAAGGCGCGCTGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATTCCTAACTCCAGCGCGTCATCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGAt                                                                                                                                                                                                                                         <  1:296629/237‑1 (MQ=255)           gCCTTTGTTAAACACCAGATTGGCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGAt                                                                                                                                                                                                                                         >  2:421077/1‑251 (MQ=255)           gCCTTTGTTAAACACCAGATTGGCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGAt                                                                                                                                                                                                                                         >  1:94090/1‑251 (MQ=255)                                         cAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAACGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCCATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATgcgc                                                                                                                                                                                                           >  2:99141/1‑251 (MQ=255)                                                                              aTCAGTGCCAGCATTTCAAAACATGGTGCCGGAGTCCCACCGGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTc                                                                                                                                                                      <  2:94090/251‑1 (MQ=255)                                                                                            ttCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATc                                                                                                                                                         <  1:402517/250‑1 (MQ=255)                                                                                               aaaaCATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGCGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGATCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATc                                                                                                                                                         >  1:193103/1‑247 (MQ=255)                                                                                                aaaCATTGTGCCGGAGTCACACATACCGCAAACGCGCGCAAAGTGGCGTTATGAATCGCCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCAAGCGCGTCAGCACCGGCTTCCATTAGCGCATCGACAATTTTCACTGACTACTCACTGCCCGGATCACCGAGCGTGACGACAGGAACGAATGCACCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTACCATCAGAtt                                                                                                                                                    >  1:129579/1‑251 (MQ=255)                                                                                                    aTTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCAGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCCATGACTACTCAATGCCCGGCTCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGCTTTccc                                                                                                                                                >  2:170535/1‑251 (MQ=255)                                                                                                      tgtgCCGGAGTCACACATGCCGCAAAGGAGCGCAGAGTGACGTGTTGAGTCGTCGGACCATCCGCAAGTGGGTCGGAGAAGGGGATACCTAAATCAAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATACTTTTCAATGAATACTCAATGCACAGCTCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAAATGGGCAAACAGAGATGCGTAGAGGTCCATCAGATTAcccc                                                                                                                                               >  2:331590/1‑250 (MQ=255)                                                                                                                 cacaCCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCaa                                                                                                                                   <  1:36075/251‑1 (MQ=255)                                                                                                                        gCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCTTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACGCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAAGGCCCGGATCACCGAGCGTGACGAAAGTAACGAATGCGCCTTCTTTGCTCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCg                                                                                                                            <  1:355028/251‑1 (MQ=255)                                                                                                                            cAAAGCCGCGCAGAGCGGCATTTTGAATCATTGAGCCACCCGCCAGCCGGTCGGAGAAGGGGATACCTAACACACGCCAGTCAGCCCCGGCTTCACTTAGCGTATAGATAATTCTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGCACGATTGCGCCTCCTTTGCGCTCCTTCACCTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCACGCTTTCAAAATATCGTGa                                                                                                                         >  2:56206/1‑250 (MQ=255)                                                                                                                                      cAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGACCGGTGa                                                                                                                  >  1:426381/1‑247 (MQ=255)                                                                                                                                            ggCGTTTTGAATCGGCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATGCCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCttt                                                                                                        <  1:356505/251‑1 (MQ=255)                                                                                                                                                    gAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATcgccgc                                                                                                <  1:244970/251‑1 (MQ=255)                                                                                                                                                        cgtcgGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGAcc                                                                                            <  1:99141/251‑1 (MQ=255)                                                                                                                                                           cgGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACTAAAGGAACGAATGCGCCTTCGTTTTGCTCCTGCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCgg                                                                                          <  1:243250/250‑1 (MQ=255)                                                                                                                                                            gggCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGa                                                                                         >  1:233032/1‑250 (MQ=255)                                                                                                                                                                 aTCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGAACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAACGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGAGAAGCTGTCTTTATCTCCGCGACCGGAAAGGtt                                                                                   >  2:363602/1‑251 (MQ=255)                                                                                                                                                                     gCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAacc                                                                               >  1:413495/1‑251 (MQ=255)                                                                                                                                                                       cAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAaccac                                                                             <  1:399566/251‑1 (MQ=255)                                                                                                                                                                                       gggATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCttt                                                              >  2:167042/1‑250 (MQ=255)                                                                                                                                                                                                    ccAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTcgc                                                >  1:172174/1‑251 (MQ=255)                                                                                                                                                                                                    ccAGCGCGGCAGCACCGGCTGCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACGGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTcgc                                                <  2:233032/251‑1 (MQ=255)                                                                                                                                                                                                                 aCCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTAATCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGAAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGCCCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCAACAtttaca                                    >  2:340764/1‑247 (MQ=255)                                                                                                                                                                                                                  ccGGCTTCAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAAc                                  <  1:327428/251‑1 (MQ=255)                                                                                                                                                                                                                         cAATTAGCGTATCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATgg                            <  1:421077/250‑1 (MQ=255)                                                                                                                                                                                                                                   aTCGATAATTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGt                  <  1:129628/250‑1 (MQ=255)                                                                                                                                                                                                                                          aTTTTCAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGAt          >  1:21814/1‑251 (MQ=255)                                                                                                                                                                                                                                               cAATGACTACTCAATGCCCGGATCACCGAGCGTGACGCACGGACCGAATCAGCCTTCTTTGCGCTCCTTCACCTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGCCCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCCAGGGCCAGGTCGTGGGGGGATTcccg     >  2:65511/1‑249 (MQ=255)                                                                                                                                                                                                                                               cAATGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCa      <  1:170535/250‑1 (MQ=255)                                                                                                                                                                                                                                                  tGACTACTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAgcgc  >  1:143980/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ACTCATCAATGCCTTTGTTAAACACCAGATTGGCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGC  >  NC_000913/1316883‑1317374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑