Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,306,7360TG93.3% 96.6 / ‑6.0 32S325A (TCC→GCC) oppFmurein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base G (14/14);  minor base A (2/0);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCCTG  >  NC_000913/1306495‑1306979
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
agaccTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCtt                                                                                                                                                                                                                                             <  2:219029/247‑1 (MQ=255)
         gACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑g                                                                                                                                                                                                                                   <  1:174762/251‑1 (MQ=255)
                 gtgaggctcaccaccATCCACTACATCCGTACACCAGGGTATTTATTTCGGCAGTCCCCATACCTGATCCGGTTCTGGAGAAGAACAAACCCACCCAGTTACTGGAAGGGGAATTACCGTCGCCGTTCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCTGTGCCGGATTGCCGGGCCAGAGTGCGCGAAATCACGTCCTGTTTGGGAGGGGAGTGTCAGACACGCAGTTTCTTGCCTGAAA‑GTCGATCCg                                                                                                                                                                                                                           <  2:184450/242‑1 (MQ=255)
                           ccACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGCGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAAc                                                                                                                                                                                                                 >  2:158792/1‑251 (MQ=255)
                                 tCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTACCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGCGGGGAGGTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATa                                                                                                                                                                                                             >  2:178956/1‑249 (MQ=255)
                                      tACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGACGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAACAACAATAAGGGCTg                                                                                                                                                                                                      >  2:39063/1‑251 (MQ=255)
                                       aCATCCTAACGCCAGGGCATTAATGTCGGCAGTCCCCATACCTGATCCGGAGCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTCCCGTCGCCGATCACCCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCCATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTTTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGa                                                                                                                                                                                                     <  2:3412/251‑1 (MQ=255)
                                                             aTGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTGCTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATGGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACg                                                                                                                                                                               <  2:357759/251‑1 (MQ=255)
                                                                       tCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCAAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACACGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCAAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTACAAACAATAAGGGCTGACCGTTGTCAGCCCTTTTTAACGCTACAAGCg                                                                                                                                                                      >  2:393816/1‑250 (MQ=255)
                                                                                   aTCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTCCCGTCGCCGATCACCCCGCCTTCCGTTTTTGTTTTCCGTACCCTTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTTTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTaaaaa                                                                                                                                                                                                                  <  2:241460/194‑1 (MQ=255)
                                                                                   aTCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTaaaaa                                                                                                                                                                                                                  >  1:241460/1‑194 (MQ=255)
                                                                                             ggAGAAGAACAAAACCATCCAGTTACTGGAAGGGTAATTCCCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTTTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatgat                                                                                                                                               <  1:39063/251‑1 (MQ=255)
                                                                                             ggAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatgat                                                                                                                                               >  1:363354/1‑251 (MQ=255)
                                                                                                 aagaaCAAAACCATCCAGTTTATGGAAGGGGAATTACCGTCGCCGATCAACCCGTCATACTGTGGTGTTTTTCGGACCCGTTGCCCTAGTGCCGGCACAGAGTGGTCCAAAACACGTCCTGTCATGGAGGGGATTTGCAGAAACCCCGTTTCTTGCCTGAAA‑GTCAATCAGCTTTAAAAACAATAAGGGATGACAGTTGTCAGCCCTTTTTCACGCTAAAATCGATTATTTATTCCCGCCAGATGATATgg                                                                                                                                           <  1:66729/251‑1 (MQ=255)
                                                                                                 aagaaCAAAACCATCAAGTTACTGGAAGGGGAATTACCGTCGCCGATCAAACCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAC‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACCGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATgg                                                                                                                                           >  2:60793/1‑251 (MQ=255)
                                                                                                      cAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAg                                                                                                                                      <  1:316795/251‑1 (MQ=255)
                                                                                                         aaCCACCCGGTTCCTGGAAGGGGATTTCCCCTCGCCGATCACCCCGCCTCCCGGTTGTTTTTTCCGTACCCGTTGCCGGATTGCGGGTCCAGAGTGCGCCAAAACACTTCTTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCGAAGttt                                                                                                                                   <  1:117924/251‑1 (MQ=255)
                                                                                                                  gTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCttt                                                                                                                          <  1:158792/251‑1 (MQ=255)
                                                                                                                        ggAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTcgc                                                                                                                     <  1:197683/250‑1 (MQ=255)
                                                                                                                          aagggaagttACCGTACCCGTTCAACCCGACTTCCGATTCAGTTATCCGTACCCGTTGCCCGACAGCCGGTAAAGACAGCGCCCCAACAAGCCCTGTTCTGGAGGGGAGTTTCAGACCCACCGTTTCTTGCCTGCAA‑GTCGATCAGCTTAAACAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAACGCGATTATTTATTCCCGCCAGCTGATCTGGCACCGTTTGTGATCTTTTTCGCGGc                                                                                                                  >  2:436799/9‑251 (MQ=255)
                                                                                                                                              aTCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCaaa                                                                                               >  1:327895/1‑250 (MQ=255)
                                                                                                                                                     cGCCTTCCGTGTGTGTTTTCAGTACCCATTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGCGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGCCAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATACCAGAATGCGGGCAAAAACATCGt                                                                                       >  2:246666/1‑251 (MQ=255)
                                                                                                                                                      tccTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACACCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAACCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCCCGCTAAACGCGATTATTTATTCCCGCCAGCTGATATGGCAAAGTTTGTGATCTTTTTCGCGTCATAACAGAATGCGTGCAAAAACATCGtt                                                                                      >  2:254115/2‑251 (MQ=255)
                                                                                                                                                       ccTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTg                                                                                     <  1:325859/251‑1 (MQ=255)
                                                                                                                                                             ggtggtgtTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCa                                                                               <  1:50567/247‑1 (MQ=255)
                                                                                                                                                                tttgtttttcgctcccGTGGCCGGATTTCCGGTCCAGGGTGAGCCTAATCACGTCCTTTTCTGGAGTGGAGTTCCATACACGCCGTTTCTTGCCTGAAA‑TTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTGGTCAGCCCTTTTTCACGCTAAAAGCGTTTATTTATTCCCGCCAGATGATATTGCAAAGTTTGTGATTGTTTTCGCTGCATAACAGAATGCGGGCAAAAACATCATCTATTTCAcc                                                                             <  2:252429/237‑1 (MQ=255)
                                                                                                                                                                                gTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGcc                                                             >  1:205606/1‑250 (MQ=255)
                                                                                                                                                                                      cGATTGCCGGTCCAAAGTGCGCCAAAACCCGTACTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCAGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGCCCGCCAGACCa                                                       >  2:383116/1‑250 (MQ=255)
                                                                                                                                                                                                tCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAC‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTc                                            >  2:85038/1‑251 (MQ=255)
                                                                                                                                                                                                                             tGGAGGGGAGTTTCCGACACGCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAAt               >  1:106573/1‑251 (MQ=255)
                                                                                                                                                                                                                                aGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAC‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCa             >  1:41822/1‑250 (MQ=255)
                                                                                                                                                                                                                                          agaccacTCCGTTGCTTGCCTGAAATGTC‑ATTCGCTTTAAAATCAATGTGGGCGGACAGTTGTCCTCCCGTTTTCACGCTAAACGCAATTAGTTATTCCCTCCGGATGATTTGGCCAAGTTTGTGATCTTTTGCGCGTCATAACAGAATGCGGGCAAAAACATCGTTGAGTCCACCTTCTTCACTGTCCGCCAGTCCAATCACCACTTCGGCAAAAAAGTCCGGTGTCAAATCGAAGTCAACATGTTCCTg  <  2:3957/247‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
AGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAA‑GTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCCTG  >  NC_000913/1306495‑1306979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑