Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,298,624	C→T	P9P (CCC→CCT)	ychE →	MarC family putative inner membrane protein YchE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,298,624	0	C	T	100.0%	66.0 / NA	19	P9P (CCC→CCT)	ychE	MarC family putative inner membrane protein YchE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/7);  total (12/7)

CATCGCTTTTCGCCATTATAGCTAACAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTT  >  NC_000913/1298385‑1298812                                                                                                                                                                                                                                                |                                                                                                                                                                                             cATCGCTTTTCGCCATTATAGCTAACAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCa                                                                                                                                                                                    >  1:189025/1‑250 (MQ=255)         ttCGCCATTATAGCTAACAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTc                                                                                                                                                                            <  1:345512/250‑1 (MQ=255)                     gCTAACAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTg                                                                                                                                                               <  1:27369/251‑1 (MQ=255)                        aaCAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTgcgc                                                                                                                                                            >  1:272069/1‑251 (MQ=255)                               aataaATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCa                                                                                                                                                      >  1:168272/1‑250 (MQ=255)                                    aTTGTAGTACGATTTGGAGGCCACATTACCATGTTTTGCACAACTAGATAACAATACCGAATGATAGACCTTTTACGTAGTTAGGAGGGGAAAAATGCTGTCAAAAGACGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGCTTCAGACCTTTTTTGATTTTCATGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCgt                                                                                                                                                >  2:31331/1‑250 (MQ=255)                                     ttGTAGCATGATTTGGTGGCTACATTAGCATGATATGCTCAAATCGATAACAATAACGAATGATAACAATTTTAAGTAGATAGGGGATGCTAAATGCAGTCCAAAAGCATATTGTCAGCGCGTCTTTTCCACCATATTTATGGCTAACATTATCCGCCTTTAGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGCTATTCCTGTTTACTTCACATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGt                                                                                                                                               >  1:353762/1‑251 (MQ=255)                                           taTGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGat                                                                                                                                         >  1:415322/1‑251 (MQ=255)                                                  tggtggCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGAGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTccc                                                                                                                                  >  2:331932/1‑251 (MQ=255)                                                                                              gAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGTCCAGTTATCAGACAGCGGCAGCGCGaa                                                                                       >  2:116102/1‑250 (MQ=255)                                                                                               aaTGATCGGAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCACAAGACGTATTGACAGCGCGTATTTTCAAACTTATTTATGGCTAACATTATCCGACTTCTGATTCGGAGCTAACCGTGATTCAGACCTTTTTTGCTTTTCCTGTTTACTTCAAATTTTTCATCGGGATATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGAGGCAGCGCGAAAc                                                                                     >  2:408953/1‑251 (MQ=255)                                                                                                              aaGTAGTTAGGAGGAGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAAACTTATTTCTGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGCTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTAc                                                                       >  2:400724/1‑250 (MQ=255)                                                                                                                     tAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTg                                                                                                                                                               >  2:107736/1‑155 (MQ=255)                                                                                                                     tAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTg                                                                                                                                                               <  1:107736/155‑1 (MQ=255)                                                                                                                                           aaaGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTtgtg                                         <  1:9438/251‑1 (MQ=255)                                                                                                                                                              gTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCTTGATTCAGACCTGTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGGCTGTGGCCATTATCTTGTTGATCTCGCTTTGTCTCGGc                      <  2:272069/251‑1 (MQ=255)                                                                                                                                                                     cAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGa               <  1:243016/251‑1 (MQ=255)                                                                                                                                                                            agttatGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCTGCGCTAAACAAAACTAACCTTGCAGCCAACCTGTCTGTGTCCATGATCTTGTGTATCTCGGTTTTTCTCGGCGACACGATTCTAc         <  2:214529/248‑1 (MQ=255)                                                                                                                                                                                  ggCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACtttt  >  2:316529/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                             CATCGCTTTTCGCCATTATAGCTAACAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTT  >  NC_000913/1298385‑1298812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑