| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,295,985 | C→T | A713T (GCG→ACG) | adhE ← | fused acetaldehyde‑CoA dehydrogenase and iron‑dependent alcohol dehydrogenasealdehyde/alcohol dehydrogenase AdhE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,295,985 | 0 | C | T | 97.0% | 103.2 / NA | 34 | A713T (GCG→ACG) | adhE | fused acetaldehyde‑CoA dehydrogenase and iron‑dependent alcohol dehydrogenasealdehyde/alcohol dehydrogenase AdhE |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (16/16); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCAGCAGTACGGTCGCCCGGTGCGCTCAGACCCAAGTGGTCGGCAATTTCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTCGGCATGTCCATAACCAGGTT > NC_000913/1295743‑1296213 | agcagcAGTACGGTCGCCCGGGGCGCCCAGACCCAAGTGGTCGGCAATTCCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATAccc < 2:231035/250‑1 (MQ=255) aCGGTCGCCCGGTGCGCTCAGACCCAAGTGTTCGGCAATTTCAGCATAACGACGGCGAGCGTGCGGACGGTCATACGTGCTTAATGCAGTCTGCTTGGTCGGGTTGTCGTTCTCATTGTAGCGAATAACTTTACAAATCAGCAGGGTGTTTGCTAGACCGTGCGTAATAGGGAACTGGGAACGCAGTTGGTGCACCATGGGGTGACATACCCTCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCg < 1:198009/251‑1 (MQ=255) gcTCAGACCCAAGTGGTCGGCACTTTCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAacac > 2:36394/1‑251 (MQ=255) aCCCAAGTGGTCGGCAATTTCAGCATAACGACGGCGAGCATGCGGACGGTCATAATGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCCGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCAc > 1:285189/1‑251 (MQ=255) caagtcggcGGCAATTGCAGCATAACGAAGGCGAGCCTGCCGACCGTCAAACTGGCTGACTGCAGTCAACTTGGTCGGGCTGTCGTTCACATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTCTGCCAGACCGTCCGGAATATGGAACTGCGCACCCAGTATGTGCGCCATTGAATGACATACACGCAGGAGGTCGTTCGCAACCGCGCTACCAGCGATAGTTGCTGCACTGTGCACACgctaccccg > 2:253117/9‑243 (MQ=255) aaGTGGTCGGCAATTTCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGATGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACgcgc > 2:316110/1‑251 (MQ=255) ggCAATTTCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGGTCGCAAACGTGATACCCGCGATAGTCGCTGCGCTGTGAACACGTTCACGCGTTACCGGAt < 2:70870/251‑1 (MQ=255) aaTTTCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGAtttt < 2:299505/251‑1 (MQ=255) tttCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGAttttt > 1:329435/1‑250 (MQ=255) ggCGAGCCTGCGTACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGAACGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGACGAAGCACTGTGAACACGTTCACGCGCTACCGGCTTTTTAGTCCCTTCGTggc > 2:226392/1‑248 (MQ=255) gCCTGCGGATGGTAACACGGGCTGAATGCACCCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGACAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCAAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGCCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGc > 2:277516/1‑251 (MQ=255) ccTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCt > 1:23257/1‑251 (MQ=255) ccTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCt < 1:36394/251‑1 (MQ=255) tCATACTGGCTGAATGCCGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACACAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTAGCTGCACtgt > 2:58701/1‑190 (MQ=255) tCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACtgt < 1:58701/190‑1 (MQ=255) gCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTc > 1:435432/1‑249 (MQ=255) gcgtGGTCGGGTTGTCGTTCGCATTGTAGCGAAGAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACCCCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCt < 2:329435/246‑1 (MQ=255) cGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGACTATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATc > 2:397614/1‑250 (MQ=255) aTTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGCGTGACATACACCCAGGAAGGCGTTCGCAAACGTGCTACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACt > 1:300563/1‑251 (MQ=255) gAATAACGTTTCAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGGTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCGCTGTGAACACGTTCGCGCGCTACCGGAGTTTTATGCCCTGCGGGGTAGGACGCTGACGGATATTCTGTCAGCAGTTTCAGTTCCTGCAGTGCCGGACCATCAGAGAACTCAGATGcc < 2:285189/251‑1 (MQ=255) ttACAACTCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGGGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCCAACGTGCTACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACACTTCGGGGTAGGACg > 2:286994/1‑178 (MQ=255) ttACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGATTGTGCGACATAGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGGGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACg < 1:286994/178‑1 (MQ=255) cagcagGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGAAACATAAg < 1:397614/251‑1 (MQ=255) agGGCGTTTGCCATACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTTACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGTATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGATACATAAGCTTc < 2:282132/251‑1 (MQ=255) gggCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACTCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTTCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGAAACATAAGCTTcc < 1:429554/251‑1 (MQ=255) gTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGAAACATAAGCTTCCATGGCGTGAGTTACTg > 1:281861/1‑251 (MQ=255) gTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGAAACATAAGCTTCCATGGCGTGAGTTACTg > 1:198043/1‑251 (MQ=255) aaTATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCa < 1:220919/251‑1 (MQ=255) atatGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAg < 1:277516/251‑1 (MQ=255) atGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGac > 1:257497/1‑251 (MQ=255) tGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGa > 1:346247/1‑250 (MQ=255) gctccATTGAGTGACACACACCCAGGAATGCGTGCGCTGACGTGATAACCGCGATAGTCGCGTCGCTGTGAGCACGTTCACGCGCTACCGGATGTTTAGACCCGTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATGAGAGAACTCAGATGCCA‑GTACAGAAACATAAGCTTCCATGGCGTGAGGTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTc < 2:355443/248‑1 (MQ=255) gcCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTCgg < 1:307357/251‑1 (MQ=255) cAGGAAGGGGTGCGAAAACGAGATGCCCGCGAAAATAGCAACACTGTGCACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGAATGGCGCGGGCAGATCTTCTTTCAGCATTTTCAGGGACTGCGGAGCCTGACCCTCAGAGAGCTCCGGT‑CCATGGACAACACCATAAACTTCAATGGCGTGAGCTCCAGCGTCCAGACCCACGAACGCACACAGGACCATCGGCGTGTCCATaatctggtc > 2:298090/1‑242 (MQ=255) | AGCAGCAGTACGGTCGCCCGGTGCGCTCAGACCCAAGTGGTCGGCAATTTCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCA‑GTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTCGGCATGTCCATAACCAGGTT > NC_000913/1295743‑1296213 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |