Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,294	C→A	noncoding (35/85 nt)	tyrV ←	tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,294	0	C	A	100.0%	35.7 / NA	15	noncoding (35/85 nt)	tyrV	tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/7);  total (8/7)

GACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCA  >  NC_000913/1287051‑1287507                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                      gtcTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGc                                                                                                                                                                                                                <  1:46493/249‑1 (MQ=255)            gTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAATCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGAAGATTTAAAGTCTGCTCCCTTTGGcc                                                                                                                                                                                                     >  1:20143/1‑251 (MQ=255)                      tggGGGCAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCTCTCTCGCTGACGCTCGAGTCGAACCTTGGTCGAAGCTTCTCCTCCTTCCCCGCATGGGCAGAATATTTAATTGCGCATTCGTT‑GGGACGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGACTGATTCGACCCTTCGAAGACGATGACGGCTGATTTAAAGTCTGATCACCTTGTCCGCTCggcaa                                                                                                                                                                                            >  2:183588/1‑247 (MQ=17)                      tggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAAc                                                                                                                                                                                           >  1:363243/1‑251 (MQ=14)                       gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAAct                                                                                                                                                                                          <  1:143570/251‑2 (MQ=255)                             aGGATTACGCTGCGCCGCTCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTccacca                                                                                                                                                                                    <  2:228538/251‑1 (MQ=255)                                     ccacggcTGCGCGCTTCGCGCTGCGGGGCGTTGCCTGCGGTAACGGTCTCTCGCTGGCGCTCGAGTCTAACCGTGGTCGAATCTTCTGATCCTTCCCCGCAGGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAATTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAAt                                                                                                                                                                            <  2:411323/246‑1 (MQ=25)                                                                           ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGAGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGCGATGGAGGTggggg                                                                                                                                       >  1:285458/1‑250 (MQ=255)                                                                                                                              tCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCa                                                                                    >  1:14144/1‑250 (MQ=255)                                                                                                                                         aTGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGACGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCCCGc                                                                        >  2:106519/1‑251 (MQ=255)                                                                                                                                                    atTTAATTGAGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTACCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCATATTTACCGGCAATGCTTGTTATCGTGGTGGGGGACGGATTATTCGTCGCTTCGCTACTCACTCTTCGGGCCGGTGCCTGTGGCTAGGTGCTCTCGCTTTCGCtcga                                                              >  2:414552/1‑250 (MQ=21)                                                                                                                                                        aaattagcgcttagtattaaggTTCAGGTATTTTTGAAAGTGATGGTGGTGGGGTAAGTATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCGCCCTTTGGCCGCTCGGGAAATCCGCCAGGGGTAATACATATTGTGAGGTTATGCTAGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCtcgaatc                                                           <  2:212887/229‑1 (MQ=25)                                                                                                                                                                   cGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAATTTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGAGGCAACGTTCTCTCGCTTTCGCTAGAATCGAACCTTAGt                                                 >  1:236445/1‑248 (MQ=255)                                                                                                                                                                                                       ggttggggAAGGAGTCGCACCTTCGAAGTCGATGACGGCAGTTTTCAAGGCTGCTCCCGTCGGCCGCTCGGTAACTCGAGCAGTGGTATGTCAAATTCGGAGGTATTGCTAGTGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCGCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACttt            <  2:360421/246‑1 (MQ=255)                                                                                                                                                                                                                aGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTGCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCa  <  1:368489/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                      GACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCA  >  NC_000913/1287051‑1287507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑