Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,278,959	C→T	L335L (CTG→TTG)	narK →	nitrate:nitrite antiporter NarK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,278,959	0	C	T	100.0%	77.5 / NA	23	L335L (CTG→TTG)	narK	nitrate:nitrite antiporter NarK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/10);  total (13/10)

TGCTGTATCTGGCAACCTTCGGCTCCTTCATCGGCTTCTCCGCGGGCTTTGCAATGCTGTCAAAAACGCAGTTCCCGGATGTTCAGATTCTGCAATACGCTTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAGGGGGTTCTGACGAACGTGCGATGCGTGAAGCGGCAACCGACACGGCGGCGGCGCTGGGTTTCATC  >  NC_000913/1278738‑1279186                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    tgctgcATCTGGCAACCTTCGGCTCCTTCATCGGCTTCTCCGCGGGCTTTGCAATGATGTCAAAAACGCCGTTCCCGGATGTTCAGATTCTGCAATACGCTTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCCGGTGGTGCATTATCTGAACGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGGCTGACGGGc                                                                                                                                                                                                          >  2:439681/1‑249 (MQ=255)              aaCCTTCGGCTCCTTCATCGGCTTCTCCGCGGGCTTTGCAATGCTGTCAAAAACGCAGTTCCCGGATGTTCAGATTCTGCAATACGCTTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCa                                                                                                                                                                                           <  1:334686/251‑1 (MQ=255)                    cGGCTCCTTCATCGGCTTCTCCGCGGGCTTTGCAATGCTGTCAAAAACGCAGTTCCCGGATGTTCAGATTCTGCAATACGCTTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCg                                                                                                                                                                                      <  1:143935/250‑1 (MQ=255)                      gCTCCTTCATCGGCTTCTCCGCGGGCTTTGCAATGCTGTCAAAAACGCAGTTCCCGGATGTTCAGATTCTGCAATACGCTTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGttc                                                                                                                                                                                   <  1:342569/251‑1 (MQ=255)                          cTTCATCGGCTTCTCCGCGGGCTTTGCAATGCTGTCAAAAACGCAGTTCCCGGATGTTCAGATTCTGCAATACGCTTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTcg                                                                                                                                                                               >  1:395472/1‑251 (MQ=255)                                         cgcgGGCTTTGCAATGCTGTCAAAAACGCAGTTCCCGGATGTTCAGATTCTGCAATACGCTTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGgcgc                                                                                                                                                                >  1:435074/1‑251 (MQ=255)                                                    cAATGCTGTCAAAAACGCAGTTCCCGGATGTTCAGATTCTGCAATACGCTTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGAc                                                                                                                                                      >  1:281382/1‑250 (MQ=255)                                                          tGTCAACAACGCAGTTCCCGGCTGTTCAGATTCTGCAATACGATTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGCTGGCGATTTTCAGCGGCCTGTTGTTCATGACCTTACCGACTGTCGGGCAGGGCGGCAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAgctggg                                                                                                                                               >  2:182096/1‑251 (MQ=255)                                                                                 gTTCAGATTCTGCAATACGCTTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTcc                                                                                                                         >  2:262457/1‑250 (MQ=255)                                                                                   tcgtaTTCTGCGATTCGCGTGCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTGCTGCAGGTGGTGCATTATCTGACCGTGTGGGCGGATCTCTTGTCACGCTGGTGAACTTTATTCTGATGGCGATGTTCAGTGGCCTGTTGTTCCTGTCCTTAGGGACTGACTCGCAAGGCGGAAGCTTCATGTCGTTCTTGGCGTTCGTCCTGGCGCTGTGCCTGGCATCTGGGCTGGTTAGGAGTTCTATTTTCCAg                                                                                                                       <  2:401821/246‑1 (MQ=255)                                                                                                        cttcGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGGGGTGCCTTATCTGGCCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGGTCTTCCGt                                                                                                  >  2:300532/1‑250 (MQ=255)                                                                                                          tcGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTGCAGTGATCTTCCGTa                                                                                                 <  1:339796/249‑1 (MQ=255)                                                                                                                 gTTTATAGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTAACGCTGGTGAACTGTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGCACTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGAGGTCTACCTGCCGCTGTTCCTGCAAGCTGGGCTGTGTAGTGATTCCACTTCCCAGATGGTTTCAGGGTTCTTcctgaacgtgcc                                                                                          >  2:40087/1‑239 (MQ=255)                                                                                                                   ttATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATg                                                                                      >  1:2502/1‑251 (MQ=255)                                                                                                                        gttGCGCTGGCGCTCTCTGCCGGGTGTTCATTGTCTGACCGTCTGGGCGGAACTCGGGTCGCGCTGGTGAACTTTATTCTGATGGCGATTTTCGGCGGCCTGTTTGTCCTGTCCTTGCCGACTGCCGGGCTGGGCTTAAGCTTCTTGTCGTTCTTCGCGGTCTGCCTGGCGCTGTTCCTTACATCTTTGCTTGGTAGTGTTTCCTCTTGCCAGATGATTGCAGTGATCTTCCGTAAACTCACAATGGATc                                                                                  <  2:192717/248‑1 (MQ=255)                                                                                                                        ggTGCGCTGGCGCGTTCTGCAGGTGGTGCAGTATCTGACCGTCTGGGCGGATCTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCTGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGTTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATcg                                                                                 <  1:436944/251‑1 (MQ=255)                                                                                                                              gtGGCGTGTTCTGCAGGTGGTGCATTATCTGACCGTCTGTGCGGAACTCGTGTCTCGCTGGTGCACTTTATGCTTATGGATATTTTCAGCGGCCTTTTGTTCCTGTCCTTACCGACTGACGGGCAGGGCGGAAGCTTCAGGGCGTTCTGCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGaa                                                                           <  2:233458/250‑1 (MQ=255)                                                                                                                                          gCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAggggg                                                               <  1:439681/251‑1 (MQ=255)                                                                                                                                               tggtgCCTTATCTGAACGTCTGGGCCGAAATCGTGTCACGCTGGTGAACTTTACTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCATCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAGGGGGTTCTg                                                          >  2:391204/1‑251 (MQ=255)                                                                                                                                                                   tGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTATGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGAGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAGGGGGTTCTGACGAACGTGCGATGCGTGa                                       >  1:400286/1‑250 (MQ=255)                                                                                                                                                                       cGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAGGGGGTTCTGACGAACGTGCGATGCGTGAAGCgg                                  >  1:363714/1‑251 (MQ=255)                                                                                                                                                                           aCTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAGGGGGTTCTGACGAACGTGCGATGCGTGAAGCGGCAAc                              <  1:262457/251‑1 (MQ=255)                                                                                                                                                                                                       tGATGGCGATTTTCAGCGGCCTGTTGTTCCTGACCTTACCGACTGACGGGCAGGTCGGCAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAGGGGGTTCTGACGAACGGGCGATGCGTGAAGCGGCAACCGACCCGGCGGCGGCCCTGGGtttactc  >  2:140507/1‑247 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    TGCTGTATCTGGCAACCTTCGGCTCCTTCATCGGCTTCTCCGCGGGCTTTGCAATGCTGTCAAAAACGCAGTTCCCGGATGTTCAGATTCTGCAATACGCTTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAGGGGGTTCTGACGAACGTGCGATGCGTGAAGCGGCAACCGACACGGCGGCGGCGCTGGGTTTCATC  >  NC_000913/1278738‑1279186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑