Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,272,743	C→T	C79C (TGC→TGT)	chaC →	glutathione‑specific gamma‑glutamylcyclotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,272,743	0	C	T	97.3%	111.3 / NA	39	C79C (TGC→TGT)	chaC	glutathione‑specific gamma‑glutamylcyclotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (21/15);  minor base G (1/0);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGA  >  NC_000913/1272509‑1272988                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      gATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGAccgcc                                                                                                                                                                                                                                        <  1:86214/250‑1 (MQ=255)                         tGCCGATAGTAAACCGGCAATTGGTGCCATTGAAGAATAACTATTCTGGTCAGCAGAACAACGGGCGGCTTCGCTGGCAGCGAAGCTGGCTTGTCGACCTGATGAGGGACCGGCGTGGAACTTCGGCCATGGATCGTTGATGCAGAATCCGGCACTGGAGTTTACCGCATCGTGCACAGGTACAATGGTTGGAAGGCATCGCGCATTTTGGCGCCGCCTGACCGCCGGGCGCGGGACGGCGAACCAGCCgg                                                                                                                                                                                                               >  2:401676/1‑251 (MQ=255)                          gCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCggg                                                                                                                                                                                                              >  1:38344/1‑251 (MQ=255)                           ccGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGCCCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGa                                                                                                                                                                                                             <  2:124202/251‑1 (MQ=255)                                tGTAAAAGGGCATTGGGTGCCATTGAAGCATCACTCTTATGGTCAGCAGAACAACGGGCGGATTAGCTGGCGGCGCCGCTGGCTTGTTGACCTGATGAGGGACCGGTGTGGATCTTCGGGTGTGTGGCGTTGATATGGAATCCGGCACTGGAGTTTACCGAATCGTGGACCGGTGCACTGGATGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGAGGGAGTGACCACCACACGGGACGGAt                                                                                                                                                                                                        >  2:209824/1‑251 (MQ=255)                                   aaaaCGGCATTTGGTGCCATTGAAGAATCCCTCTTATGGTCATCAAAACAACGGACGGCTTCGCTGGCGGAAACGCTGGCTTGACGACCTGCTGAGGGACCGGAGTGGATCTTCGGCTTTGGATAGTTGATGTGGAATCATACACGGGAGTTTACTGAATCGTGCACCGGTACAAGGGTTGGATGGCCTCGCGAATCTTGTCTGCGCATGACCACCGATCGCGGCACAGCGCACCACCCGTGACGGAtcat                                                                                                                                                                                                     >  2:118693/1‑248 (MQ=255)                                                     aTTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCgg                                                                                                                                                                                   >  1:237756/1‑251 (MQ=255)                                                                        ggTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGGAAGAGGGCGGACGCACCACAGGCGGCGcc                                                                                                                                                                >  2:88360/1‑251 (MQ=255)                                                                               agAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGCTCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACCCTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGCAAGAGGGCGGACGCACCACCGGCGTAGCCTATCGAc                                                                                                                                                         >  1:392114/1‑251 (MQ=255)                                                                                    aacGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGcc                                                                                                                                                     >  1:155402/1‑250 (MQ=255)                                                                                    aacGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCa                                                                                                                                                    <  2:182900/251‑1 (MQ=255)                                                                                     acGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCag                                                                                                                                                   <  1:73933/251‑1 (MQ=255)                                                                                                cGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTg                                                                                                                                        >  1:221902/1‑251 (MQ=255)                                                                                                                  cTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCtg                                                                                                                      >  1:368909/1‑251 (MQ=255)                                                                                                                                    gtcctgtgtGGATCTTCGGTTATGGATCGTTGATGTTGAATCCGGCATTGGAGTTTTCCGAATCGTGCACCGGTTCACTGGTTGGATGTCAGCGCGAATCTTGCCTGCGCCTAACCGGCGGGCGGTGGACTTCGCTCCAGTCGGGACGGATGATTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGTCTACCGACTGCCAGAAGAGACGCTGGAGCATGAACTAACCCTGTTGTGGAAGCGTGAgatt                                                                                                    <  2:233199/246‑2 (MQ=255)                                                                                                                                         gtgtGGATCTTCGGGTACGGATCGTTGATGGGGAATCCGGCAATGGAGTTTACCGAATCGTGCACCGGTACAGTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCAGGCGCGGGATTGCGCACCAGCCGGGACGGATGCTTGCACTGAACGAGGGCGGACGCACCACAGGCGTAGCCTATCGACTGCCAGCAGAAACGCTGGCGCAGGAACCAACCCTGATGTTGAAAGGAGAGAGGATTAc                                                                                               >  2:321681/1‑251 (MQ=255)                                                                                                                                                   taggCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCTTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGAGGCTTGCACTGAAAGAGGGCGGCCGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGTGCAGGAACTAACCCTGTTGTGGAAGCGAAGGATGAATACCGGCTGtttg                                                                                     >  2:326614/3‑249 (MQ=255)                                                                                                                                                   tCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCTCCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTACACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGtt                                                                                       <  1:15058/249‑1 (MQ=255)                                                                                                                                                   tCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAACCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGCCTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCTTAGCGACGGCCATAAGAGACGCTGGAGCAGGAACTAGCCCTGTTGTGCAAGCGAGATATGATTACCGGCTGtt                                                                                       <  2:215088/249‑1 (MQ=255)                                                                                                                                                     ggCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCt                                                                                   >  1:402805/1‑251 (MQ=255)                                                                                                                                                      gCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTg                                                                                  <  1:287509/251‑1 (MQ=255)                                                                                                                                                                      gtgGAAGCCGGCACTGGAGTTTACCTAATCGTGCGCCGGGACACTGGTTGTATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTTTCAGc                                                                  <  2:373698/251‑1 (MQ=255)                                                                                                                                                                           aTCCGGCACTGGAGTTTACCGAATCGTGCAGCGGTACACTGGTTGGATTGCATCGCGCATTTTGACTGCGCCTGACCGCCGGGCGCGGGACTGGGCACCAGCCGGGACGGATGCTTGCACTGAAAGATGGGGGACGCACCACAGGCGCCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGcttgat                                                             <  1:38191/251‑1 (MQ=255)                                                                                                                                                                              cGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGcttgatctt                                                          <  1:382617/251‑1 (MQ=255)                                                                                                                                                                                   cTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTtgatg                                                      >  1:60089/1‑250 (MQ=255)                                                                                                                                                                                    tGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTgatgat                                                    >  1:251900/1‑251 (MQ=255)                                                                                                                                                                                     ggAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTtgatga                                                     <  1:88360/249‑1 (MQ=255)                                                                                                                                                                                            aCCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTTCCCAACTGGTGTCAACTTGATATTGATGATTGACGcac                                            >  2:430038/1‑251 (MQ=255)                                                                                                                                                                                             ccGAATCGTGCATCGGTGCACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGTCCGGCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTTGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGcaca                                           <  1:355458/251‑1 (MQ=255)                                                                                                                                                                                             ccGAATCGGGCACCGGTACACTGGTTGGACTGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGcaca                                           <  2:368909/251‑1 (MQ=255)                                                                                                                                                                                                              ccacTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGGCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGtt                          <  1:53985/250‑1 (MQ=255)                                                                                                                                                                                                                 cTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTttat                       >  1:102796/1‑251 (MQ=255)                                                                                                                                                                                                                      tGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAAACTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATgg                  >  1:101090/1‑251 (MQ=255)                                                                                                                                                                                                                      tGGAGGGCATCGCGCAGTTTGTCTGCGGCTGACCGCCGGGCGCGTGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCAGAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTGGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGAGGATGGACGCACAGTAAACGCCATTGTGTTTATTATg                   <  2:187820/250‑1 (MQ=255)                                                                                                                                                                                                                       ggATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAgatgat                                                                                                  >  1:76883/1‑170 (MQ=255)                                                                                                                                                                                                                       ggATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAgatgat                                                                                                  <  2:76883/170‑1 (MQ=255)                                                                                                                                                                                                                       ggATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACACCATTGTGTTTATTATgg                  >  1:434947/1‑250 (MQ=255)                                                                                                                                                                                                                             cATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCcgcg           >  1:426317/1‑251 (MQ=255)                                                                                                                                                                                                                                       tttGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGa  >  1:7776/1‑250 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      GATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGA  >  NC_000913/1272509‑1272988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑