Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,836	A→G	L180P (CTG→CCG)	ldtC ←	L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,836	0	A	G	95.7%	77.8 / NA	23	L180P (CTG→CCG)	ldtC	L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (11/11);  minor base T (0/1);  total (11/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCC‑TGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTC  >  NC_000913/1169605‑1170047                                                                                                                                                                                                                                         |                                                                                                                                                                                                                    aCCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCC‑TGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGc                                                                                                                                                                                                  <  1:425033/251‑1 (MQ=255)  ccAGACGCTCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCTCTTTGGTGCC‑TGGGGGGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTCCCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCa                                                                                                                                                                                                 <  2:343694/251‑1 (MQ=255)                 ttGGGTCGGAAGAGACTTTTAGCGGAGTGTTGATAATATTCACTTTGGTGCC‑TGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGACCTTCCCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCATATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTTTCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTcc                                                                                                                                                                                   <  2:38087/250‑1 (MQ=255)                      gcGGCAGAGACTTTTATCGGAGTGTTGAGGATATTCACTTTGGTGCC‑TGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCAGCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAAGGCCGAAAGCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCTGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGc                                                                                                                                                                             <  2:261805/250‑1 (MQ=255)                                  tttATCGTAGTGGTGATGATATTACCTTTGGGGCCGTGGGG‑GACCGGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGAGGAATACAGCCAGAACTTACCCGCAGGCCAAGGCCGAAAGCGGCGTTCGTACAATGAAGCAAATAACCGCCGCCCTAGGCCGCCAGACGAATCGCATGATGGCCCATGGGGTTATCCGGGCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACggg                                                                                                                                                                  <  2:14080/250‑1 (MQ=255)                                                                            gACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATACCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGATGGCGTCCAGGATGGGTTTGctcgat                                                                                                                        >  2:221120/1‑246 (MQ=255)                                                                                                 gATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACGCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCa                                                                                                   <  1:244920/251‑1 (MQ=255)                                                                                                  atatCGTCCTCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCAt                                                                                                  >  2:352965/1‑251 (MQ=255)                                                                                                                 aGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTtat                                                                                                                                                                        <  1:196606/166‑1 (MQ=255)                                                                                                                 aGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTtat                                                                                                                                                                        >  2:196606/1‑166 (MQ=255)                                                                                                                 aGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCgt                                                                                   >  1:297657/1‑251 (MQ=255)                                                                                                                 aGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCgt                                                                                   >  1:33961/1‑251 (MQ=255)                                                                                                                 aGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCgt                                                                                   >  1:53027/1‑251 (MQ=255)                                                                                                                                              gCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTg                                                      <  1:307582/251‑1 (MQ=255)                                                                                                                                                     ccGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCAGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGCTacac                                               >  1:204764/1‑251 (MQ=255)                                                                                                                                                                gTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCACATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGAAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAAt                                    >  2:118268/1‑251 (MQ=255)                                                                                                                                                                       ccATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTAcc                             <  1:352965/251‑1 (MQ=255)                                                                                                                                                                               cAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGgt                     <  1:409182/251‑1 (MQ=255)                                                                                                                                                                                        gccgccATAGGCCTCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATGGGATACACGTTTACCGAATTTTTACCCGGCGGGTAGTAATaga            <  1:142301/251‑1 (MQ=255)                                                                                                                                                                                        gccgccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATaga            <  1:160587/251‑1 (MQ=255)                                                                                                                                                                                        gccgccATAGGCCGCCAGACGAATCGCATGATGGCACCTTGGGTTATCAGGTCCAGCAGGAACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGATGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCACTACATATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAAt               >  1:244078/1‑248 (MQ=255)                                                                                                                                                                                            ccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACg        >  1:33059/1‑251 (MQ=255)                                                                                                                                                                                                  gccgccAGACGAGTCGAATGCTTGCCAGTTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCCATTCCCTGAGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCCGGTTGGATTTGCACGTTTGTCTGAAACGGTGGTCCCCATTGTCGGTGTCAGCGTGTCACCACCTACATGACCAATACCTATTGGATACACAGTAACAGCATTTTTAACCGGCGGGTAGTAAAAAAGACGCAGCTc  >  2:33956/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                    ACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCC‑TGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTC  >  NC_000913/1169605‑1170047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑