| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,098,620 | T→A | V245V (GTT→GTA) | ghrA → | glyoxylate/hydroxypyruvate reductase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,098,620 | 0 | T | A | 92.9% | 88.8 / ‑0.5 | 29 | V245V (GTT→GTA) | ghrA | glyoxylate/hydroxypyruvate reductase A |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base A (15/11); total (17/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
GAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGGAA > NC_000913/1098380‑1098859 | gAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCa > 1:96657/1‑250 (MQ=255) gCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGGTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTa < 1:30476/250‑1 (MQ=255) ccGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGt < 1:380250/251‑1 (MQ=255) gCATTTCTGAGCAAATGTCGGGTATTTATTAATTTGTTACCGAATCCCCCTGAACCCGTCGGCATTATTAATCAACAATTACTAGAAAAATTACCTGAGGGCGAGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATATCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGACCCCTTACCGCCTGAAAGTCCGCGCTGGCAACATCCAc < 2:227828/250‑1 (MQ=255) tGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAAcacc > 1:205000/1‑251 (MQ=255) aaTTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACCCCACATGTCGCcgcgct > 2:411358/1‑249 (MQ=255) ccccTGAAACCGTCGGCATTATTAATCAACAAATACCCGAAACATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGCGGAAGATGACCTGCTAGCGGCGCTGGATAGCGGCAACGTAAAAAGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGa > 1:191384/1‑250 (MQ=255) aaCCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTgg < 1:178618/251‑1 (MQ=255) cGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCCATTACCCGTCCCGCTGCAGCTGTGGAGTAc > 2:353674/1‑250 (MQ=255) aaTTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGaaaa > 2:79479/1‑250 (MQ=255) ttACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAgg > 1:265670/1‑251 (MQ=255) tGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAggg > 1:23738/1‑251 (MQ=255) ggCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGAACTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCGTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACGCCTCATGTCGCCTCTATTACCCGTCCCGCGGAAGCGGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAATGGGAGAggtt < 2:259248/251‑3 (MQ=255) gcgTATCTCCTCAACCGGGCTCGTGGTGTTCATGGTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATTTGTTTAACCGTGAACCCGTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGTAGCGGTGTAGTACATTTCTCGCACCATTGCCCAGCTCGACAAAGGGGAGAGTGTCTg < 2:199070/251‑1 (MQ=255) gTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTAc < 1:162879/180‑1 (MQ=255) gTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTAc > 2:162879/1‑180 (MQ=255) gTATCTCCTCAACCAGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTATGCg > 1:101231/1‑251 (MQ=255) cctcAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCaa < 2:96657/250‑1 (MQ=255) cTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTCCTCGCGGCGCTGGATAGCGGCAAAGTACACGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCCCGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACcg > 2:439083/1‑251 (MQ=255) catgttccggcaGATGACATGCTCGCGGCGTTGGCTCGCCGCACCGTCAACGGCGCAATGTTGGGTGCTTTTAATCGTGAACCCTTACAGCCTGCAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGACGATGTGGAGAACATTTCTCGCACCATTGCCCAGCTCGACCAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGctact > 2:388747/12‑250 (MQ=255) tgGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGc > 1:321284/1‑251 (MQ=255) tGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCCCGCGGCTACTAATAAAGCATCAGGATTCCt > 2:380494/1‑251 (MQ=255) tGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATACACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGATGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGAGGGCAAGTCGAcc > 1:263812/1‑216 (MQ=255) ggcgCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATcc < 2:265670/251‑1 (MQ=255) cgcTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGTATGTTTTTAATCGTGAACCCTTACCGCCTGAAATTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCTCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCtt < 1:221965/251‑1 (MQ=255) aGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAGGCATCAGGATTCCTGCTATCCTTGGCGGGaa < 1:439083/251‑1 (MQ=255) aGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCACTCTGGCAACATCCACGAGTGACGATAACACCAAATGTCGCCGCGATTACCCGTCCCGCTGAAACTGTGCAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGCGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGGa > 1:396927/1‑250 (MQ=255) aGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGACCCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCa > 2:217956/1‑143 (MQ=255) aGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCa < 1:217956/143‑1 (MQ=255) | GAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGGAA > NC_000913/1098380‑1098859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |