Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	968,837	G→A	D158N (GAT→AAT)	lpxK →	tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	968,837	0	G	A	97.0%	103.6 / NA	34	D158N (GAT→AAT)	lpxK	tetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (12/20);  minor base C (1/0);  total (14/20)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGT‑CCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGG  >  NC_000913/968594‑969071                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            aTTCGCGTGGGGGTGGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGGTTACAGCATTACCGTCTGGCGCGTAATGTGGa                                                                                                                                                                                                                                      <  2:205773/251‑1 (MQ=255)     gcgggggggTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTTTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACTCCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAAtt                                                                                                                                                                                                                                  <  1:133578/247‑1 (MQ=255)               cGTATCGCGGGGATATGGTTGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTAtt                                                                                                                                                                                                                         <  1:146893/250‑1 (MQ=255)               cGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAAACCGGGTTGGTTTTTTCACGCCCTTATGCGCCTGTTTCGGGTTTTCCCGTTCGTTCTTGTTCGGTAAAAGCCCTTCTGGCGCCACACCCTGTTGTGCAGATCATCCTAACCGCAGACGGGTTACCACCTTACCGTCTGGCGCGGTAAGTAGAAATTGTAGTTAttt                                                                                                                                                                                                                        >  2:304468/1‑250 (MQ=255)                gTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGGTTTATCAAAGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAACCATTCTGGCGCAACACCCTGATGTGCAGGTCATCGTAACCGACGACGGTTTACAGCCTTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTgt                                                                                                                                                                                                                       >  1:87636/1‑250 (MQ=255)                      gggggATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGAGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGgtgt                                                                                                                                                                                                                 <  2:133611/250‑1 (MQ=255)                      cgGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGgtgt                                                                                                                                                                                                                 <  1:368121/251‑1 (MQ=255)                                       ggCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATg                                                                                                                                                                                                <  1:23933/251‑1 (MQ=255)                                         cTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGATGATGAACCTTTGTTGGTTTTTCAAAGCACTGATGCGCCTGGTGCGGGTTCTCCCGTTCGTTCTGATGCGGGAAAAGCCCTTCTGGGGCAACACCCTGCTTTGTAGAATATTGTAACCGCCAGCGGTTTAAAACAATACCGTCTGGCGCGTCATGTGGGAATTGTCGTGTTTGATGGtgtggggcggcttgggaatggg                                                                                                                                                                                              >  2:311361/1‑233 (MQ=255)                                                                                      aGGCGGGGGATGAAACTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGCCGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGGGGGCGAGCggggggcgtt                                                                                                                                                 >  2:158510/1‑245 (MQ=255)                                                                                              gatgaACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCCATGCGGGGGCGAGGGGGGGGCTTTAAGGCggg                                                                                                                                         >  2:202543/1‑250 (MQ=255)                                                                                                 gaACCTGTGTTGATTTATCAACGCACTGATGCGCCGGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGGTTACAGCATTACCGTCTGGCGCGTAATGTGTAAATTGTCTTTATTGATGGTGTGCGTCGCGTTGGCAAGGGCTGGTGGTTTCCGGCGGGGGCAAGTTGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGa                                                                                                                                      <  1:21603/251‑1 (MQ=255)                                                                                                    ccTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCCATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGGTGa                                                                                                                                      >  1:237571/1‑248 (MQ=255)                                                                                                    ccTGTGTTGATTTATCAACGCACTGAGGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGa                                                                                                                                      <  2:237571/248‑1 (MQ=255)                                                                                                       gtgtTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCGGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAAGTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGAGGTTGCCGGCGGGGCCAAGGCGGGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGt                                                                                                                                <  1:366907/251‑1 (MQ=255)                                                                                                                       gCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGGGGGCGAGCGGGGGGCTGAAAGTCGGGTGATGCGGGAAACGGAAAcggcggg                                                                                                                >  2:115077/1‑250 (MQ=255)                                                                                                                        cACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGGGGTTGCCGGCGGGGCGAATGGGTGAGCGAGCGGGGGGCTTTAAGTCGGTTGCTGCGGAAATCGTCAAcggggggg                                                                                                               >  1:358126/1‑246 (MQ=255)                                                                                                                             aTGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTCATCGTCCAcggcgggggcccc                                                                                                          >  1:429915/1‑244 (MQ=255)                                                                                                                               gcgcCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGT‑CCCTc                                                                                                        <  1:25624/250‑1 (MQ=255)                                                                                                                                    tGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGGTCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGACATTTTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGGGGTTGCCGGCGGGGCCCATTCGTTGGCGGGCGGGGGGCTTTAAGTGGGTTGATGGGGTTATCgccacccggcgggagcctcgccggg                                                                                                   >  2:225840/1‑227 (MQ=255)                                                                                                                                                  ccgtgctttcTGATGCGGTAAACGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGT‑CCCTCGCAGCGGTTAAATCCCCATg                                                                                    <  2:401336/244‑1 (MQ=255)                                                                                                                                                             gATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGT‑CCCTCGCAGCGGTGAAATCCCCATGCATctgct                                                                            <  1:49667/248‑1 (MQ=255)                                                                                                                                                                      aaaGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGGGG‑CCCTCGCAGCGGGGGAATTCCCATGCATCTGCGGCCggggtaggg                                                                >  1:67516/1‑245 (MQ=255)                                                                                                                                                                                 ggCGCAACACCCTGATGTGCAGATCATCGTAACCTACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGTCTGGTGGTTGcc                                                                                                                                                                                   <  2:372331/126‑1 (MQ=255)                                                                                                                                                                                 ggCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGcc                                                                                                                                                                                   >  1:372331/1‑126 (MQ=255)                                                                                                                                                                                 ggCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGT‑CCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTAc                                                     <  1:202543/251‑1 (MQ=255)                                                                                                                                                                                           ccTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGGCAACGGCGG‑GTGCCCTCGCAGCGGGGGAAACCCCATGCATTCGCTGCCGGGGCAGGCgggggaatttcggaacgggac                                           >  1:155944/1‑232 (MQ=255)                                                                                                                                                                                                 gtgCAGATCATCGTATCCGACGACTGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCTTTATTGATGGTGTGCGTCTCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCTGTTGATGCGGTAATCGGCAACGGCGGTGT‑CCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGAACCGGTACGCGtt                                      <  2:215536/250‑1 (MQ=255)                                                                                                                                                                                                   gCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTAGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCACTTCGTGAACGAGCGGGGCGCTTAAAGGCGGTTGCTGCGGTAATCGTCAACGGCGGCGG‑CCCTCGCGCCGGGGTAATCCCCATGCATCAGCTGCCGGGGCAGGCGGGGaaatttagcaacagcaagcggttgg                                   >  1:297298/1‑228 (MQ=255)                                                                                                                                                                                                             gTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGT‑CCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGGACCGGTACGCGTTGTGACGTTGCTCa                         <  2:46230/251‑1 (MQ=255)                                                                                                                                                                                                                  cgacgaCTGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTTGCAATGGCTGGTGGTTGCCGGCGGGGCCAAGGCGTGAGCGAGCGGTGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGT‑CCCTCGCTGCGGTGAAATCCCCGGGCATCTGCTTCCGGGTCGGGCGGTGAATTTACGGACCGGTACGCGTTGTGACGTTGCTCAGCTTg                    <  1:279054/251‑1 (MQ=255)                                                                                                                                                                                                                                 gCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGT‑CCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAATGGCGa     <  1:114920/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTTACCGTCTGGCGCTTAATGTGGAAAGTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGT‑CCCTCGCAGCGGTGAAATCCCCATGCATCTGCGGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGTCGATg   <  2:363518/251‑1 (MQ=255)                                                                                                                                                                                                                                     tACCGTCTGGCGCGTAAGGTGGAAATTGTCGTTATTGAGGGTGGGCGTCGCTTTGGAAATGGATGGTGGTTGCCTGCGGGGCCCATGCGTGAGCGCGCGGGGCGCTTAACGTCGGTTGATGCGGTAATCGTCAACGGCGGTGT‑CCATCGCAGCGGTGAAATCCCCATGCATTTGATGCTGGGTAAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGTTCAGCTTGAACATGTAGTGGTGATgg  <  2:141412/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            ATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGT‑CCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGG  >  NC_000913/968594‑969071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑