BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,038	G→A	H97Y (CAT→TAT)	umpH ←	UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,038	0	G	A	95.1%	139.2 / NA	41	H97Y (CAT→TAT)	umpH	UMP phosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/17); minor base C (2/0); total (24/17)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTAC  >  NC_000913/699800‑700266

ttACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGCTAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGATTCACATCGGTAATAGTAAAACCGGCTTTGTACAGTTCATAAATCAGTGCGcc                                                                                                                                                                                                                          >  1:423992/1‑251 (MQ=255)

  aCCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGAGCGCCt                                                                                                                                                                                                                         <  1:116004/250‑1 (MQ=255)

            aaCGTTTTGCGCCCGGAGAGTTTCTCAATCTCTGCACTCAACGCGCCCCAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGGCCCAGTTGTAGGAACGCGTTTCGCCAACAAGCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCAGAAATCAGTGCGTCTTCGCCcaccac                                                                                                                                                                                                              <  2:83298/251‑1 (MQ=255)

              cGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACAt                                                                                                                                                                                                            >  2:419187/1‑251 (MQ=255)

               ggTTGGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGTCGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGATCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATa                                                                                                                                                                                                           <  2:161442/251‑1 (MQ=255)

                gTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGCTCATAAATCTGTGCGCCTTCGCCAACCACATAc                                                                                                                                                                                                          >  2:303625/1‑251 (MQ=255)

                               ttttCTTAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTcc                                                                                                                                                                                           >  1:122752/1‑251 (MQ=255)

                                          ccTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAg                                                                                                                                                                                <  1:75859/251‑1 (MQ=255)

                                             gCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGGCCCATGTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTGGTACATTTCATAAAGCAGTGCGCCTTCGCCCGCCACATACGCTTTCGTGCGTTCCTGGCGACGCAgg                                                                                                                                                                               <  2:35324/249‑1 (MQ=255)

                                                        gcCACAAGCTGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCg                                                                                                                                                                  <  2:308281/251‑1 (MQ=255)

                                                           acaAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGCAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGAAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCATGGCGACGCAGGAAATCGGAAGTCGccc                                                                                                                                                               >  2:286318/1‑250 (MQ=255)

                                                                 gggATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGAGATCAAACGGACACCGTTAGCGACGCAATAGGCTGCTTTATGAATACTGTCACAGTTATAGGAACGCGTTTCGACAACAATCACGAACTCAGGGTTCACATCGGTAATAGTGACACCGGATTTGTACAGTTCATAAATCAGAGCGCCTTCGCCCACAACATACGCTTTCTTGCCTTCCTGGAGACGCAGGAAATCGGCATTCGcactcgc                                                                                                                                                           >  2:359267/1‑243 (MQ=255)

                                                                     tAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGt                                                                                                                                                     >  1:350923/1‑251 (MQ=255)

                                                                      aaaaaCCGCGACCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAACCCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAgga                                                                                                                                                     >  2:134583/1‑249 (MQ=255)

                                                                             gctcccGTGGGGGTCCGGATTTGGGGCGAAAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCTCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACTGTTCATAAATCAGTGCGCCTGCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGTCGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAact                                                                                                                                             <  2:22386/248‑2 (MQ=255)

                                                                                 ccGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGGGCGCCGTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGt                                                                                                                                                                    <  1:178563/224‑1 (MQ=255)

                                                                                 ccGTGGGCGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGACCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATCAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGt                                                                                                                                                                    >  2:178563/1‑224 (MQ=255)

                                                                                  cGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTg                                                                                                                                        >  1:77358/1‑251 (MQ=255)

                                                                                      ggTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTc                                                                                                                                      >  1:158905/1‑249 (MQ=255)

                                                                                         gtCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGAATAAAACACGCTGTCAGGt                                                                                                                                  >  1:352327/1‑250 (MQ=255)

                                                                                            cGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACAt                                                                                                                              <  1:267619/251‑1 (MQ=255)

                                                                                             ggATTGGTGGCGATAAAACGTGCACCGTTAGCGACGCAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCAAATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCCGGTAAATc                                                                                                                             >  2:110728/1‑251 (MQ=255)

                                                                                                    tggCGATCAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGACCCAGTTGTAGGAACGCGTTTCGCCAACAATCCCGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCCGTCGCCATCGCAGAGGTATAAAACACTCTGTCAGGTACATCGACAccc                                                                                                                      >  2:222883/1‑250 (MQ=255)

                                                                                                     ggCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGGTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTg                                                                                                                     >  1:376143/1‑251 (MQ=255)

                                                                                                       cGATAAACCGTGCACAGTTAGCGACGAAATCGGCTGCTTTCTGCCTCCTGTCCCAGATGTAGGCACGCGTTTCGCAAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCACACCACCTACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCTCCCTCGCAGAGGTATAAAACCCGCTGTCAGGTCAATCGacacagctc                                                                                                                   >  2:249847/1‑246 (MQ=255)

                                                                                                       cGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCAGCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGGGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGTCAGGCGCCATCGCATAGGTATAAAACACGCTGTCAGGTACATCGacac                                                                                                                        <  1:249847/246‑1 (MQ=255)

                                                                                                                      cGTTAGCGACGAAATAGGCTGCCTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCCTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTc                                                                                                    >  1:140771/1‑251 (MQ=255)

                                                                                                                            cgacgaAATAGTCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGTCAGTCGCGATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGGTCGTCAGa                                                                                              <  2:292181/251‑1 (MQ=255)

                                                                                                                             gacgaAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGcc                                                                                                 >  1:59396/1‑247 (MQ=255)

                                                                                                                                 aaaTAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTg                                                                                         >  1:11546/1‑251 (MQ=255)

                                                                                                                                 aaaTAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTGCGCCCACCACATACGCTTGCGTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCGAGCGCAGAGGTATAAAACACGCTGTCTGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTg                                                                                         <  1:155459/251‑1 (MQ=255)

                                                                                                                                                        ggcccAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTg                                                                   <  1:303625/248‑1 (MQ=255)

                                                                                                                                                        gTCCCAGGTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGATCGCCAGATCTTGCCCAGTCGGCGAAGGATAGTTgg                                                                  >  2:308066/1‑251 (MQ=255)

                                                                                                                                                         tCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGt                                                                 >  1:131497/1‑251 (MQ=255)

                                                                                                                                                                                gccaaacatcccGAAATCAGGTTTCACATCGGTCATAGTGAAACCGGCTTTGTACAGTTCATCAATCAGTGCGCCTTCGCCAACCAAATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCCGTCGCCATCGCAGAGGCATAAAACACGCTGTCAGGTACATCGTCACCTGCGATGGCAAAGCGGTTCGCCACATCTTGCCCCGTCTGCGAAGGATAGTTGGTCAGCAACCCCCGCGGCAGGCCtt                                          >  2:396507/12‑251 (MQ=255)

                                                                                                                                                                                      aattaCGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCATTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCa                                    <  2:30395/247‑1 (MQ=255)

                                                                                                                                                                                                              ggTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTg            >  1:295122/1‑251 (MQ=255)

                                                                                                                                                                                                              ggTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCAACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAAACGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATAGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTGTATCCATAATACCGTGCAAAAAtaacgctg            >  1:305920/1‑244 (MQ=255)

                                                                                                                                                                                                                taagaGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCg                                                                                                                                                            <  1:394950/101‑1 (MQ=255)

                                                                                                                                                                                                                     gTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCgg     <  1:107653/251‑1 (MQ=255)

                                                                                                                                                                                                                        aaaCCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACAGCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTAc  <  2:158905/251‑1 (MQ=255)

TTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTAC  >  NC_000913/699800‑700266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`