Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	657,282	G→A	intergenic (+165/‑10)	pagP → / → cspE	Lipid A palmitoyltransferase/transcription antiterminator and regulator of RNA stability CspE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	657,282	0	G	A	100.0%	59.9 / NA	18	intergenic (+165/‑10)	pagP/cspE	Lipid A palmitoyltransferase/transcription antiterminator and regulator of RNA stability CspE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

AGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATT  >  NC_000913/657047‑657519                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              aGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCt                                                                                                                                                                                                                                <  2:8063/251‑1 (MQ=255)               ccGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTaa                                                                                                                                                                                                                  <  1:423206/251‑1 (MQ=255)                  ggTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGt                                                                                                                                                                                                               <  2:168583/251‑1 (MQ=255)                            acaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTTTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGGGGttt                                                                                                                                                                                                     <  1:292391/251‑1 (MQ=255)                            acaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGttt                                                                                                                                                                                                     >  1:127851/1‑251 (MQ=255)                                                    ggATGCGCTGTCAGGTTTGAGACAAATAAATTTTTATTAACTTCTTTAAAATCAAGTGCTAAAATAAGTAACATAAAAAATAACGCGACTTTTTTCACTTTTTATAAAAGGTACCCGTGTCAATGCGTACCACAATTGGTGTACTGTTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTTAGTGGTTTAATTAGTCCAAAGGATTCGGTTTc                                                                                                                                                                             <  2:237015/251‑1 (MQ=255)                                                         cgcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTAc                                                                                                                                                                        >  2:185881/1‑251 (MQ=255)                                                                 gTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTACAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGa                                                                                                                                                                  >  1:208770/1‑249 (MQ=255)                                                                           aaaTGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGAAAAGTTACACTGGACAAAGCGTACCACAATTGGCGTACTGGTAACCGACACAGCATTTGAGTCTATTTTTAATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCAAAAGGAGTCGGTTTCATTACTCAGGAAGACGgcagca                                                                                                                                                       >  2:296835/1‑250 (MQ=255)                                                                               gAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGCCTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAGGAc                                                                                                                                                  >  2:309177/1‑251 (MQ=255)                                                                               gAAGTTTTAGGAACTTCGTTAAAATCAATTGCTAAAATAAGTAACATCAAAAATAACGCGACTGTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGAc                                                                                                                                                  <  2:172161/251‑1 (MQ=255)                                                                                                          aTAGCTCAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATcc                                                                                                                         >  2:327768/1‑249 (MQ=255)                                                                                                                   aTAAGTAACATCAAAAATAACGCGACTTTTAGCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACCCTTCTCTGCAATCCAGACTAATGGt                                                                                                              <  2:152910/251‑1 (MQ=255)                                                                                                                               aaaaaTAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTAGACAAAGCGTACCAGAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATGTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTGTAATGAGTCCAAATGTTTCGATTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGGCGAATGGTTTTAAAACACtt                                                                                                  <  2:308753/251‑1 (MQ=255)                                                                                                                                                                                              gtgtACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAGAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAAc                                   <  1:328002/251‑1 (MQ=255)                                                                                                                                                                                                           cGACACAGCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGt                      <  1:396436/251‑1 (MQ=255)                                                                                                                                                                                                                  gCATTTGTGTCTATTTTTCATGTAAAAGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTACCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATAc               >  1:132105/1‑251 (MQ=255)                                                                                                                                                                                                                               tttttCATGTAAAAGTAATTTTGATGTCTAAGATTACCGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAAAGTAATCGCTCTGTAAGATACGTCAGCAAGAAtt  >  2:363850/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              AGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATT  >  NC_000913/657047‑657519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑