Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	100.0%	177.9 / NA	51	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (26/25);  total (26/25)

AGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCT  >  NC_000913/4636679‑4637167                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCcccc                                                                                                                                                                                                                                                  <  1:5844/250‑1 (MQ=255) aGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCg                                                                                                                                                                                                                                                 <  1:163579/251‑1 (MQ=255)               gtactGCGTATCGGCATGCGGTTGTTGCTGGGCGATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCTTGCGAAGAGCAACGGAGGGGACGTTGAGCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACATCATCCCCCGTTTCGCGTCAatat                                                                                                                                                                                                                                   <  2:60877/247‑1 (MQ=255)               gTAATGCGTTTCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCATCGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAat                                                                                                                                                                                                                                     <  2:95403/249‑1 (MQ=255)               gTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAatat                                                                                                                                                                                                                                   <  1:389751/251‑1 (MQ=255)               gTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAat                                                                                                                                                                                                                                     <  1:20842/249‑1 (MQ=255)                    gCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGGTAAACCGGGTGGGCGAAGATCAACGGAGGGGACGTTGATCGACTCCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGAGTTGCTCGCTGGGGATCTAACGCATGGTCAACTGGCTCATGCGTTTAAGCAGTTACAACATCCCCCGTTGCGTGCCAATGTCGGt                                                                                                                                                                                                                               <  2:365326/250‑1 (MQ=255)                         tCGGCATGCGGGTGTTGCGGGGCTACTTTTAGCTGGTGTCGGTGGCAGCCGGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGTTGACGTTGATCGACACCGCAACGTTGCTGGCTGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGTGCAACTGGCGCAGGCGTTTAAGCAGCTACAACATCCCCCGTTTCGCGCTAATATctttgtcat                                                                                                                                                                                                                          <  2:54604/250‑9 (MQ=255)                                        tgCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACTCCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTGCGCGCCAATATCGGTGGCATTAACaaaagatcgga                                                                                                                                                                                                           <  1:49059/250‑9 (MQ=255)                                                   ttgtttcTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAACCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTTATCGACACCGCAACGTGGCTGGCGGAGCTGGCGCGTCCCGAGTTGCTCTCTGGGGACCCAACGTGTGTGCAGCGGGCGCATGCGTTTAAGCAGCTACAACATCCCTCGTTTCGCGCCTATATCGGTGTCATTAACAAAGTGCGCAATGAATATC‑ATg                                                                                                                                                                                               <  2:250766/244‑1 (MQ=255)                                                         cTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCCTTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTatat                                                                                                                                                                                        >  1:213321/1‑251 (MQ=255)                                                           ggtggcggtggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATTGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCAGTAACAAAGTGCGCAATGAATATC‑ATGTCTatat                                                                                                                                                                                        <  1:398082/249‑1 (MQ=255)                                                           ggtggcggtggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTatat                                                                                                                                                                                        <  1:342208/249‑1 (MQ=255)                                                                    tgcAGCCTGGTTCGTCCAGGCAATTTATGGCCAAGCCGTTAAACCAGGCGTGCGCAGAGCAACGGAGGGGCCGTTGATAGACACCGCAACGTTGCTGGCGGCGCTGGAGCGTCCAGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTCATCAGCTACACCAGCCCCCGTTTAGCGCCAATATCGGAGGCATTGACAAAGGGCGCACTGAATATCCAT‑TCTATATGACCGATgagc                                                                                                                                                                             >  2:60209/2‑248 (MQ=255)                                                                       aGCCTGGTTCGTACTGGCCATTGTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGGCGTTGATAGACACCGCAACGATGCTGGCGGAGCTGGCGCGTACCGATTTGCTCTCTGGGGCCCCAACGCCTGCGCAACTGGCGCAGGCGTGTAATCCGCTAACACATACCCCATTTCCAGACACTACCAGTGGCAGTCACAAAGTGCGAAATTAATATC‑ATGtatcactgaccgatgggaagg                                                                                                                                                                          >  2:239796/1‑231 (MQ=255)                                                                        gCCTGGTTCGTACTGTCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAgtg                                                                                                                                                                         <  2:10408/251‑3 (MQ=255)                                                                         ccTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGc                                                                                                                                                                        <  2:163483/251‑1 (MQ=255)                                                                          cTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑CTGTCTATATGACCGATGCGCAGGGc                                                                                                                                                                        >  2:353970/1‑250 (MQ=255)                                                                            ttttCGTACTGTCCATTTATCACAAAGAAGTTAACCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACCTCGCAACGTTGCGGGCGGAGCTGGTGCGTCCCGATTTGCTCTCTGGGTACCCAACGCATGGGCAACTGGCGCAGGCGTTTAAGCAGCTACAACATCCCCCGTTTCGCGCCAATATCGTTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCa                                                                                                                                                                       <  2:277133/247‑1 (MQ=255)                                                                               tCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGt                                                                                                                                                                   <  2:72256/250‑1 (MQ=255)                                                                                cGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTa                                                                                                                                                                  <  1:159285/250‑1 (MQ=255)                                                                                cGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTAt                                                                                                                                                                 <  1:353970/251‑1 (MQ=255)                                                                                 gTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGAGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGGACCGCTTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTCAGCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTATATATGACCGATGCGCAGGGCACAGTAtt                                                                                                                                                                >  2:112100/1‑251 (MQ=255)                                                                                   aCTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAg                                                                                                                                                                                                                  >  2:163480/1‑200 (MQ=255)                                                                                   aCTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAg                                                                                                                                                                                                                  <  1:163480/200‑1 (MQ=255)                                                                                                  cAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAAt                                                                                                                                                >  1:327349/1‑250 (MQ=255)                                                                                                    aagaagTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGGCCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCAGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATaa                                                                                                                                              >  1:267371/1‑250 (MQ=255)                                                                                                         gTTAAATCGGGCGTGCGAAGAGCAACGGAGGGGCCGTTGATCGACACAGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATc                                                                                                                                                                                                                                  >  1:115024/1‑162 (MQ=255)                                                                                                         gTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATc                                                                                                                                                                                                                                  <  2:115024/162‑1 (MQ=255)                                                                                                          ttAAACCGGGCGTGCGAAGAGCAACGGAGGCAACCATGATCCACACCGCAACGTTGCTGGCGGCGCTGGCGCGTCCCGATTTGATCTCTGGGGACCCAACGCCTGGGCAACTGGCGCCGGCGTTTAATCAGATACAACATCCCCCGTTTCGCGCCAATATCGGGGGCATTAACCAAGTGCGAAATGAATATC‑ATGTCTATATGACAGATGCGCAGGGCAAAGGATTGTTCGATTCGGCAAATAAAGACGtt                                                                                                                                       >  2:178103/1‑251 (MQ=255)                                                                                                            aaaCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTgg                                                                                                                                     >  1:417797/1‑251 (MQ=255)                                                                                                                      tGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCAGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGGGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGattat                                                                                                                           >  2:44436/1‑251 (MQ=255)                                                                                                                         gAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCCTGGGCAACTGGCGCAGGCGTTTAATAAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑CTGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTcg                                                                                                                        >  2:247598/1‑251 (MQ=255)                                                                                                                                   ggAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTCATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGACTATC‑ATGTCTATATGCACGATGCGCAGGGCAACGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATg                                                                                                              >  2:167875/1‑251 (MQ=255)                                                                                                                                       gggACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGt                                                                                                          >  1:133232/1‑251 (MQ=255)                                                                                                                                               gATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTa                                                                                                   <  1:247598/250‑1 (MQ=255)                                                                                                                                                                      cGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGCATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTgcg                                                                           >  1:40143/1‑251 (MQ=255)                                                                                                                                                                                  gTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGAATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTg                                                               >  1:178383/1‑251 (MQ=255)                                                                                                                                                                                         tttGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATc                                                        >  1:304895/1‑251 (MQ=255)                                                                                                                                                                                            gCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTg                                                     >  1:378048/1‑251 (MQ=255)                                                                                                                                                                                                 ctGGGGACCCAACGCATGGGCAACTGGCGCAGGCGGTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAAGATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGGATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCTCTGGAATGACGGCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGcc                                                   <  1:263771/248‑1 (MQ=255)                                                                                                                                                                                                 ctGGGGACCCAACGCATAGGCAAATGGCGCAGGCGATTAATCAGCAAAACCATCCCCCGCTCCGCACCAATCTCGGTGGCAATAAAAACGTGCGCAATGAATATC‑ATGTCTATCTGACCGTAGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGCCGTCTGGCTAACGTTGCGTGGTCCGTATAGTGCGCGCAGCACGTTCCCAAATCCTGCCGAt                                                >  2:424143/1‑251 (MQ=255)                                                                                                                                                                                                         ccAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGaa                                          >  1:99692/1‑249 (MQ=255)                                                                                                                                                                                                                        cTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGatgtatgt                         >  1:201665/1‑251 (MQ=255)                                                                                                                                                                                                                         gggCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGtt                        <  2:201665/250‑1 (MQ=255)                                                                                                                                                                                                                                   gtttacccAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCg               >  1:115247/8‑250 (MQ=255)                                                                                                                                                                                                                                   gTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTCTATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGCCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCgaac                 >  2:23373/1‑245 (MQ=255)                                                                                                                                                                                                                                    tttAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGGTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGAt             >  2:303870/1‑251 (MQ=255)                                                                                                                                                                                                                                      tAATCAGCTTCAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGCCCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCTAAAGTTCTGTGATGTATGTTGCCGCACCGAtt            <  2:417797/250‑1 (MQ=255)                                                                                                                                                                                                                                       aaTCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTAt          >  1:323447/1‑251 (MQ=255)                                                                                                                                                                                                                                               acaacaTCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCTCAGCACGTTGCAAAATCCTGCCGATCCCTAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCt  <  1:178103/251‑1 (MQ=255)                                                                                                                                                                                                                                               acaacaTCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCt  <  1:376138/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    AGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATC‑ATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCT  >  NC_000913/4636679‑4637167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑