| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,615,345 | C→T | G67S (GGC→AGC) | yjjW ← | putative glycyl‑radical enzyme activating enzyme YjjW |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,615,345 | 0 | C | T | 98.8% | 277.3 / ‑6.5 | 81 | G67S (GGC→AGC) | yjjW | putative glycyl‑radical enzyme activating enzyme YjjW |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (41/39); minor base G (0/1); total (41/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
GTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAAC‑AGCGCCACCACAAACGGCAGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGAATACGAGTGTTG > NC_000913/4615104‑4615584 | gTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAAC‑AGCGCCACCACAAACGGCAGCTGGGTCG‑TGGCTTCACCGCCCCTCACCGATATCCCTTCGAAAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGACTTGCGCCATGGGCGAGGCATGTTGCGGACACCTCTTCCGGCAGGTATCAAACTGCTCGCAAACCACAGCGTACCACACCACTTTGCTGTCAACAAt > 2:261415/1‑251 (MQ=255) cAGTTGCGGATCGTTTTTGATAGCAGTAAAC‑AGCGCCACCACAAACGGCAGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCGGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCt > 1:373872/1‑251 (MQ=255) cGGATCGTTTTTGATAGCAGAAAAC‑AGCGCCACCACAAACGGCAGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCCTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGCATCACAATGCTCGCAAACCACAGCGTTCCACACCACTTTGGTGTCAACAATCTGCAACGCCTGATGCg > 2:297018/1‑251 (MQ=255) gtaTCGTTTGTGATAGCA‑TAAACCAGCCCCCCCACAAACGGCAGCTGG‑TCGGTGGTTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCAGGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACGCCACTTTGCTGTCAACAATCTGCAACGCCTGATGCgg < 1:145626/249‑1 (MQ=255) ggATCGTTTTTGATAGCAGTAAAC‑AGCGCCACCACAAACGGCAGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCgg > 1:95108/1‑251 (MQ=255) gtgtttGATAGCAGTAAAC‑AGCGACACCACAAACGGCAGCTGGGTCG‑TGGCTTCACAGCCAATAACCGTTATCCCTTAGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGAGGAAACCTCTTCAGGCAGGGATCACACAGCTCGCACACCCCAGCGATCCACACCACTTTGCTGGCAACAATCTGCAAAGCCTGATGCGGACACt > 2:16953/4‑251 (MQ=255) gTTTTTGCTAGCAGTAAAC‑AGCGCCACCACAAACGCCAGCTGGGTCC‑TGGCTTCACCGCCACTCCCCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCCTGGGCGTGGCATGTTGCGACCCCCTCTTCAGGCAGGTATCCCACTGCTCGCAACCCACAGCGTTCCACACCCCTTCGCTGTCATCAATCTGCAACGCCTgcgccggacact > 2:158573/1‑240 (MQ=255) ccagaaaaC‑AGCACCCCCACAACCGGAGGCGGGGTCG‑GGGCTTCCCCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGgcacgca < 2:314216/246‑1 (MQ=255) cAGTAAAC‑AGCGCCACCACAAACGGCAGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGgcacgca > 1:314216/1‑250 (MQ=255) aGTAAAC‑GGCGCCCCCACAAACGGCAGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACt < 1:103099/251‑1 (MQ=255) gTAAAC‑AGCGCCACCACAAACGGCAGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTc > 1:204780/1‑251 (MQ=255) tAAAC‑AGCGCCACCACAAACGGCAGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTcc > 1:197943/1‑251 (MQ=255) aaaC‑AGCGCCACCCCAAACGGCAGCGGGTTCG‑GGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACGccc < 1:220485/251‑1 (MQ=255) c‑AGCGCCACCACAAACGGCAGCTGGGTCG‑TGGATTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCcac < 1:60566/251‑1 (MQ=255) aGCGCCACCACAAACGGCAGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCCTGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACCATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCcac > 2:340692/1‑250 (MQ=255) aaccggcggtggggTCG‑TGCCTCCTCCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCTCTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAAc < 1:345176/240‑1 (MQ=255) aaccGGCAGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAAc < 1:99243/248‑1 (MQ=255) ccGGCTGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGt < 1:211072/250‑1 (MQ=255) ggCAGCTTGGTCG‑GGGTTTACCCGCCACTCTCCGTTCTCCCTTCGATAAACAGCACTGCGTTGCGGACATGTCTAAGCACTGCGTCCACGCTCAGGGATTGCGCCGTGGGCGTGGCATGTTGCGGCCTCCTCTTCAGGCAGGTATCTCACTTCTCGCAACCCACAGCTTTCCACCCCCCTTTGCTGTCAACAATCTGCAACGCCTGATGCTGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTcc < 2:439287/251‑1 (MQ=255) ggtgtcttgtcTTCTCCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACTCCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTTGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGt < 1:83854/241‑1 (MQ=255) gggggggggCTTAACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCGGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTc < 2:373872/244‑1 (MQ=255) ggTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAATCAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCAAGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGAACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTcc > 1:429043/1‑251 (MQ=255) ggTCG‑AGGCTTCACCACCTCTCACCGTTAACCCTTAGATACAAAGCACTGCTTTGCGGCCATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATAGGCGTGGCATGTTGCGGAAACCTCTTCAGGCAGGTATCCCACTGCTCGCACACCACAGCGTTCCACACCACTTTCCTGTCAACCATCTGCCACGCCTGATGCGGACACTGTGGCACGCCATCCCCACAGTCATTGCAACGTCCCTTCGtac > 2:282650/1‑249 (MQ=255) gTCG‑TGGCTTCACCGCCACTCACCGTTATCCATTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTcc > 1:144897/1‑250 (MQ=255) ggtgatctccccgacACTCACCGGTATCCCTTCGTTAATCAGCTCGGCGTTACGGACATGGCTAAGCACTTCGTCCACGCTCATGGAGTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTAGCACACAGCTCGCAAACCGCAGCGTTCCACCCCACTCTGCTGTCAACAATCTGCAGCGCCTGATGCGGCCACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCTATCGTCGACg < 2:298276/237‑1 (MQ=255) gggCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGa < 1:125063/250‑1 (MQ=255) ggCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGAt < 1:412067/251‑1 (MQ=255) ccACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATtgtg < 1:43993/250‑1 (MQ=255) ccGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACa < 1:238025/251‑1 (MQ=255) ccACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGtt > 2:26802/1‑251 (MQ=255) ccTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCGGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGCCCACGGATTGTGACAGtttt < 2:74252/250‑1 (MQ=255) cGTTATCGCTTCGATAAATAGCACTGCTTTGCGGACAGGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCAAGTTGCAGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTGCACACCACTTTGCTGCAAACAATATGCAACGCCTGATGCGGACCCTGAGGCACGCATTCCCCACAGTCATTGCAACGTCCCATCGTCCCCGGATTATGACAgcgtttgaagc > 2:102284/1‑241 (MQ=255) tATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAgcgc > 1:383221/1‑250 (MQ=255) aTCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACCGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGGGGCACGCACTCCCCACAGTCATTGCCACGTCACATCGTCCCCGGATTGTGACAGTTTTTGCAgcgc > 2:154361/1‑249 (MQ=255) ccccTTCGACAAACAGCACTGCTTGGCGGACATGGCTAAGTACTTCGTCCACGCTCATGGATTGCGCCATGGTCGTGGCATGTTGCGGACTCCTCTTCAGGCAGGTATCACACTGCGCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTTTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGa < 2:355874/250‑1 (MQ=255) gATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCGCTTCAGGCAGGTATCACACTTCTCGCAAACCACAGCGTTCCTCACCACTTTGCTGCCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACTTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGAGTGCAGc < 1:387813/251‑1 (MQ=255) ttGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACaga < 2:109859/249‑1 (MQ=255) ttGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACaga > 1:109859/1‑249 (MQ=255) ttGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGc > 1:249577/1‑251 (MQ=255) tGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGc < 1:96803/250‑1 (MQ=255) gaggACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTACAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTGTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCa < 1:272712/249‑1 (MQ=255) cGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGcc > 1:105160/1‑249 (MQ=255) cGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCACTGCAAAAACAGAGcc > 2:161083/1‑249 (MQ=255) ggACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGcc > 1:27971/1‑248 (MQ=255) gACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCCCGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAgac > 2:201756/1‑251 (MQ=255) aCATGTCTAAGCACTGCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAgacg < 2:217844/251‑1 (MQ=255) gggCTAAGCACGTCGTCCACGCTCATGGATTGCGTCATGGGCGTGGCATGGTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAgacgtct < 2:422304/250‑4 (MQ=255) cTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGcc > 1:424294/1‑251 (MQ=255) gCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGc > 1:134659/1‑251 (MQ=255) cACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCAAGACGACTGCCTGGcc > 2:378980/1‑251 (MQ=255) tCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGCCACCAATTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGATGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTCCAGCCCTGCAAAAACAGAGCCAGACGCCTGCCTGGCCCGTCAAc > 1:62296/1‑251 (MQ=255) tCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACATCTTCAAGCAGGAATCACACTGATCGCAAACCACAGCGTTCCACACCCCTTTGCTGTCAACAATCTGCAACGCCTCATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAAc > 1:198825/1‑251 (MQ=255) tCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAgg > 1:375695/1‑249 (MQ=255) gATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCAGTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAggg < 1:131162/251‑1 (MQ=255) gATTGCGCCATGGGCGTGGCATGTTGCGGACACCGCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAggg < 2:105160/251‑1 (MQ=255) tGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGa < 1:378980/250‑1 (MQ=255) tGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCtt > 1:6925/1‑250 (MQ=255) gggCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTa < 2:96590/250‑1 (MQ=255) ggCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTTCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACt < 1:199325/251‑1 (MQ=255) tGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACCGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACt > 2:194830/1‑251 (MQ=255) ggCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACt < 2:197943/250‑1 (MQ=255) cATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGAGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAAAAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGCAGGGGGTAATCTTACTGACtca > 2:112053/1‑248 (MQ=255) ctTCAGGCCGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCCGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTc > 2:35180/1‑250 (MQ=255) tCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTc < 2:119822/248‑1 (MQ=255) caggcaggTATCTCACGGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATgg < 2:439735/251‑1 (MQ=255) caggcaggTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATgg > 1:31659/1‑251 (MQ=255) caggcaggTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATgg < 1:16953/251‑1 (MQ=255) ggcaggTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCt > 1:252279/1‑251 (MQ=255) tATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCAc > 1:62510/1‑251 (MQ=255) cacaCTGCTCGCAAACCACAGCGTTCCACACCACATTGCTGTCAACAATCTGCAACGACTGATGCGGACACTGTGGCACGCACTCTCCTCAGTCATTGCAACGTCCCATCGTCCACGGAGTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATctc < 2:204780/248‑3 (MQ=255) gCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCt < 1:26802/250‑1 (MQ=255) gCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGt < 2:6925/251‑1 (MQ=255) aaCCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTcc > 1:123613/1‑251 (MQ=255) ccACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTgg > 1:269234/1‑166 (MQ=255) ccACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATGGCAACGTCCCAGCTTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTgg < 2:269234/166‑1 (MQ=255) cacaGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGa < 2:301843/251‑1 (MQ=255) acaGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTTATGCGGACACTGCGGCACGCTCGCCCCACAGTCATTGCAACTTCCCATCGTCCACGTATTGTGACAGTTTTTGCAGCGCATATTGCAGCCCTTCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTAACTGACTAAAGCGCATCTCCTGGGCATGGCTTATCACGCGCGGCTGGCGTTCCAGaa < 1:282650/251‑1 (MQ=255) acaGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTTATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGa > 1:213302/1‑250 (MQ=255) cacaCCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGAATACGAgtgt > 1:46833/1‑251 (MQ=255) caccacCTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGAATACGAGTGTtg > 1:119435/1‑251 (MQ=255) accacTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAg < 1:326152/165‑1 (MQ=255) | GTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAAC‑AGCGCCACCACAAACGGCAGCTGGGTCG‑TGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGAATACGAGTGTTG > NC_000913/4615104‑4615584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |