| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,499,661 | T→C | intergenic (+161/‑1599) | insD6 → / → yjgZ | IS2 insertion element protein InsB/uncharacterized protein YjgZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,499,661 | 0 | T | C | 97.1% | 115.1 / ‑3.1 | 36 | intergenic (+161/‑1599) | insD6/yjgZ | IS2 insertion element protein InsB/uncharacterized protein YjgZ |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (14/20); minor base A (0/1); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01 | |||||||||||
GGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑TAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCC > NC_000913/4499422‑4499904 | gggTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATa > 1:266025/1‑250 (MQ=255) ggTTATCGCTCGCCACGGGAATATCTGCGGCAGCGTGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTGTTCAGGACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTGGCAATCTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAAc < 1:417729/251‑1 (MQ=38) gttGTAAAGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCTCCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGGCTGGATTACAGTCTGCCATATATGATTTCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCGCCCT‑TAAAAAGCATATCAGGTTAGTTATCTTGGTCTGTGAGCGGATTGAATGGc < 2:185108/250‑1 (MQ=255) aaTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGtat < 1:231102/251‑1 (MQ=255) aaGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGGGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGTTATATTCTTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCa < 1:251335/250‑1 (MQ=255) gTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTGCAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAACCCATAACAGGTTAGGTATCTGCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTa < 2:389755/251‑1 (MQ=255) gTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGTCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAAACCATATCAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACATATACCGGt < 2:161296/251‑1 (MQ=255) ggtaagataGGGGCATATCCACCTTGTGCGGATATGAATACGGATATTTTCTACAGAATACGTTTCTGGACAAGAGACTGTAGTACAGTCTGTCATATATGATTTCCTGTGATCAGGAAGAAGAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTAGATTCCTCGCTCCCT‑CAAAAGCCATAACATGTTAGTTATCTTCGTCTGTCAGCGGATTGAATAGCGGTATATTTTCAGTTCAAATACCGGTCATcc < 2:14513/245‑1 (MQ=255) tataGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACTGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGGGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAAACATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACACATACCAGTCATCCACTgg > 2:80962/1‑250 (MQ=255) aGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGaaaa < 1:100426/251‑1 (MQ=255) tGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGTAAAATACCTGCGAAATGAAGGGCACTGAt > 2:224773/1‑251 (MQ=255) ttttATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGACATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTACCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGAc > 1:204500/1‑251 (MQ=255) ataCAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACggg < 2:182617/250‑1 (MQ=255) gAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCtgatg < 1:267247/251‑1 (MQ=255) gAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCtgatg < 2:296214/251‑1 (MQ=255) aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCtgatga < 1:146567/251‑1 (MQ=255) aTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCtgatg > 1:200608/1‑249 (MQ=255) cAGTACAAGCGACTGAATTCCAGTCTGCCATCTATGATTACCTGTGATCAGAAAGAATCAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGAATATTTTCAGGACAAATACCGGTCATCCACTGAAAAATAACTGCGAAATGACGGGCACTGATTTTTCAACTGACGGGCTGATGAGACGTTATg > 2:398525/1‑251 (MQ=255) cAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTGTCTACTGACGGGCTGATGAGACGTTATGTCACt < 1:173243/251‑1 (MQ=255) aaGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTg < 1:54444/251‑1 (MQ=255) cGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGAc > 1:256711/1‑223 (MQ=255) cGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCg > 1:238412/1‑251 (MQ=255) cGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACGGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAGTGACGGGTACTGATTTTTCTACTGAc < 2:256711/223‑1 (MQ=255) gTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGCTTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGAGGTATTAATc > 2:92069/1‑247 (MQ=255) tGCCATCTATGATTACCTGTGATCAGAAAGAATAAGTGGCTGAGGTTTCAAACAGTCTGACGGCATATTCAACTCTCCCA‑CAAAAACCATAACAGGTTAGTTATCGTCGTCTGTCAGCGGATTGAATGGCGGAATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCAATGATTTTTTTACTCACGGGCTAGTGGGCAGTTATGTCACTAGCGGtgaatattgagggtg > 2:274611/1‑237 (MQ=255) ccATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGcaca < 1:224773/251‑1 (MQ=255) atatatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGATATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGAAGGGCACTGATTTTTCTAATGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATACGTGGCacacc > 1:309074/1‑250 (MQ=255) gATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACCGGCGGTAATAATCAGTGGCACACTGTAGc < 1:355493/250‑1 (MQ=255) gtgATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGca < 1:105677/251‑1 (MQ=255) ggAAGAATAAGTGGCTGAGGTTTCAAACGGTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGAcc < 2:200608/251‑1 (MQ=255) ggAAGAATAAGTGGCTGAGGTTTCAAACGGTCTGCCGGTATAGTCCTCGCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTAGATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACGGACGGGCTGATGAGACGTTATGTCACTGGCGTTAATAATCAGTGGCACACTGTAGCCTCCCTGCCCATGAcc < 2:407069/251‑1 (MQ=255) gTCTGCCGGTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGAGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTc < 2:217580/249‑1 (MQ=255) ggTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGAATATTTTCCGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGCCGTTATGTCACTGGCGGTAAAAAACTGGGGCACACCGTCGCCTCCCTGCACATGCCCAAAATTATGAACAGAATTGacacatgcgctgtacgc > 2:297254/1‑238 (MQ=255) gTATATTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCCCCCTGCACATGACCACAATGATGAACCAGATTGGCCCTGTCGCTGACCGAc > 1:167186/1‑251 (MQ=255) tatTCCTCTCTCCCT‑CAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTCGCCTACCTGCACATGACCCTAATGATGGACAGGATTGGCCCTGTCGCTGACCGAccgc > 1:305397/1‑248 (MQ=255) cccT‑CAAAAACCATAACATGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTGCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACGGTAGCCTCCCTGCGCATGACCATAATGATGAACAGGATTGGCATTTTCGCTGTCCGACAGCCCAAggtc < 2:435044/248‑3 (MQ=255) | GGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCT‑TAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCC > NC_000913/4499422‑4499904 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |