| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,396,772 | T→C | F236F (TTT→TTC) | amiB → | N‑acetylmuramoyl‑L‑alanine amidase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,396,772 | 0 | T | C | 96.0% | 247.4 / NA | 76 | F236F (TTT→TTC) | amiB | N‑acetylmuramoyl‑L‑alanine amidase B |
| Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (32/40); minor base A (2/1); total (35/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGC > NC_000913/4396529‑4397014 | aCCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCg > 1:297752/1‑251 (MQ=255) ccGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGt < 1:431442/251‑1 (MQ=255) cgcgcAATCCGGTTAAAACGGAAAGTAACCGCACTACGTGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTTGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGGCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAGAATGTCACC‑ATCGCCAGCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGGTCAAAGGCGtttt < 2:407934/250‑1 (MQ=255) gcAATCCGTTTAAAACGGAAAGTAACCGAACTACGGGTGGTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAAAACTGGCGATAAAAGTATCATCGCTAATGATGCCGGACACGGCGGGCAGGATCCTGGCGCTATCGGCCACGGTGGTAAGCGGGAGAAAAATGTCACC‑ATCGACATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGAGCCGATGTTCAAAGGCGTTTTaaac < 2:240767/251‑3 (MQ=255) gcAATACGTTTAAAACGGCAAGTAACCGCAATACGGGTGTTATCAACAGTAATACGGTAACGCGTACGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATAATCGCTATTGATGCCGGACACGGCGGTAAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAAC‑ATCGCCATCGCGCGGAAA‑TTGCGTACTTTGCTGAATGACGATCCGATGTTAAAAGGCGTTTTCAcc > 2:64968/1‑251 (MQ=255) cAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAAccc < 1:167666/251‑1 (MQ=255) ttAAAACGGAAAGTCACCGCACTACGGGTGTTATCAGCAGAAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGACGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGCGCAAAATGTCACC‑AACGCCATCGCGCGTAAA‑TTGCCGACCTTGCTAAATGACGATCCGATGTTACAAGGCGTTTTAACACGTGCCggg > 1:358483/1‑251 (MQ=255) aaaCGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGAt < 1:104671/251‑1 (MQ=255) aaaGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACttt > 1:336023/1‑251 (MQ=255) aaaGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATGATCATCGCTAGTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATTTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGTTCCTATGTGCAAAGGCGTTTTAACCCGTGACGGGGATTACttt < 1:278352/251‑1 (MQ=255) aaGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGTCGTTTTAACCCGTGACGGGGATTACTTTa < 1:33014/251‑1 (MQ=255) aCCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGGTTTAACCCGTGACGGGGGTTACTTTATCTCg > 1:141594/1‑251 (MQ=255) cACTACGGGTGTTATCAGCAGTAATACGGTAGCGGGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑GTGCGTACTTTGCTCAATGACGATCCGAGGTTCAAAGGCGTTTTAACCCGTGACGGGGATAACTTTATCTCGGTGa < 2:209371/251‑1 (MQ=255) tACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATgg > 1:274455/1‑251 (MQ=255) tgtTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCCGGCGCTATCGGACCCTGTGGTACGCGGGCGAAAAATGTCCCC‑ATCGCCATCGCGCGTAAA‑TTGCGGACTTTGCTCAATGACGATCCGAATTTCAAAGGCGTGTTAACCCGTGACGGGGGCTACTTTATCTCGGGGGTggggagcg > 2:431750/1‑247 (MQ=255) tATCAGAAGTAATACGGTAACGCGTCCGGCAGCGAGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGGGGGACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGGAAA‑TTGCGTACTTTGCTCAATGAAGATCCGATGTTCAAAAGGGTTTTAACCCGTGTCGGGGATTACTTTCTCTCGGGGCTGGGGCGCAGTg > 1:306807/1‑251 (MQ=255) tAATACGGTAACGCGTCCGGCAGCGCGCGCGCCGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAAGGCGGTCAGGCCCCTGGCGCTATCGGCCCCGGTGGTACGGGGGAGAAAAAT‑TGACCCATCGCCATCGCGCGTAAA‑TTGCGGACTTTGCTAAATGAAGATCCGATGTTCAAAGGCGTTTTAACCCGTGGCGAGGTTTAATTTCTCTCGGTGATGGGGCGCCGTgcgtggggac > 2:50295/1‑242 (MQ=255) tAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGGGATGTGGCAc > 1:251021/1‑251 (MQ=255) aaTACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTGTATCTCGGTGATGGGGCGCAGTGATGTGGCACg < 1:64968/251‑1 (MQ=255) tACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTCTCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGt > 1:199778/1‑250 (MQ=255) tACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGt > 1:269121/1‑250 (MQ=255) cGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCCATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGGGGCACGTa > 1:234390/1‑249 (MQ=255) cgcgTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGCTGCCGGACACGGCGGTCAAGACCCTGGCGCTCTAAGCCCCGGAGGCACGCGGGCGAAAAATGTCACC‑CACCCCATCGCGCGTAAA‑TTACGATCTTATATCAATGAAGGTTCGAGGTTTAAAAGGGTTTTAACACGGGCCGGGGATTACTTTTTATAGGGGATGGGGCGCAGTGATGTGGCACGGAAGCaaaa > 2:3544/1‑250 (MQ=255) ccGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCaa < 1:434455/251‑1 (MQ=255) ccGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCCCTATCGGCCCCGGTGGTACGCGGGCGAAAAATGTCCCC‑ATCCCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCCCGTAAGCAAAACGCCa > 1:247184/1‑250 (MQ=255) gCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGTCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑GTGCGTACTTTGCGCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGGGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAAttt < 1:275163/251‑1 (MQ=255) gcgcgcgcGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCt < 1:223522/251‑1 (MQ=255) gcgcgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCCGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCg > 1:169456/1‑251 (MQ=255) cgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTCAGCAAAAAGCCAATTTCCTCgtgt > 1:48971/1‑251 (MQ=255) ggCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGc > 1:199124/1‑251 (MQ=255) ggCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAAGACCCTGGCGCTCTCGGACACGGTGGTACGCGGCAGGCAAATGTCACC‑CTCGCCAACGCGCGTAAA‑TTGCGTCATTTGCTCCATGACGATCCGCTGTTCCAAGGCGGTTTTACCCGTGACGGGGATTACTTTTTCTCGGTGATGGGGCGCAGGGCTGAGGAAAGGCAGCAAACCGCCCATTTCCTCGTGTCGCTTCACCCTggtgg > 2:409919/1‑247 (MQ=255) gATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCCCGACCCTAGCGCTATCGGCCCCGGAGGTAAGCGGGAGAAAAATGTCACC‑ATCGCCATCGAGCGTAAA‑TTGCCTACATTGATCACTGACGAAACGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGGGATGGGGCGCAGTGATGTGGCACGTAAGCACAACGCCAATTTCCTCGTGTCGGTTCACGCTGATTCCGc > 1:196628/1‑251 (MQ=255) aaTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGGGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGAACCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGGGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGGTGCCGCACCg > 2:340092/1‑250 (MQ=255) aTTATCAGCGCTAGTTAGGCCGGGCACGGCGGTCAGGACCCTGGCGCGATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGAGCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCTCGTAAGCAAATCGCCAATTTCCTCGGGTCGATTCACGCTGATTCCGCACCGaa < 2:392417/251‑1 (MQ=255) aacaccgctatagaggacGGACACGGCGGTCGGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGGAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGGTAAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAAcc < 2:100110/233‑1 (MQ=255) tcatcGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCACCGGTGGTACGCGGGAGAAAAATGTACCC‑ATCGACCTCGAGCGTAAA‑TTGCGTACTTTAATCAATTAAGATCCGATGTTCAAAGGCGTTTTAACCCGTGAAGGGGATTACTTTATCTCGGTGGTGGGGCGCAGTGATGGGGCACGTAAGAAAAAAGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGaccagc > 1:81129/1‑246 (MQ=255) atcGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTAtctc < 1:42837/167‑1 (MQ=255) cGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGt < 2:13690/250‑1 (MQ=255) gATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTgg < 1:324961/251‑1 (MQ=255) gATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTg < 2:199124/250‑1 (MQ=255) gATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTg < 1:341515/250‑1 (MQ=255) ggcggTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑GTCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCATGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATg < 1:101495/251‑1 (MQ=255) gTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTg < 1:348492/251‑1 (MQ=255) tGGCGCTATAGACCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTCCA‑TTGCGCACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTACCCCGTGACGGGGATTACTTTATCTCGGAGATGGGGCGCAGTGATGTGGCACGTACGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGCTGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTCTGGGCGCTCTCTaccc > 2:117346/1‑248 (MQ=255) gtcgcgaTCGGCCCCTGTGGTTCGCGGTAGAAAAATTTCGCC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTGGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCTGTTATGTGGCACGTAAGCAAAACGCCAATTGCCTCGTGTCGATTCTCGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTatcc < 2:320230/244‑4 (MQ=255) cgcTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCGCC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTc < 2:84550/251‑1 (MQ=255) gcTATCGGCCACTGCGGCACGCGGGAGAAACATGTCCCA‑ATCGCTCTCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGTTGGGGCGAAGTGCTGTGGCACGTAAGCAAAACGCCAATTTCCTCGAGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGAGACTGGCGCTTCCTTATGACTGCTCTCTAACCGt > 2:415385/1‑249 (MQ=255) ggCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCa > 1:18891/1‑250 (MQ=255) cccGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAAc > 1:225845/1‑249 (MQ=255) cGGTGGTACGCGGGCGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTg > 1:78160/1‑251 (MQ=255) ggtggtACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTg > 1:43120/1‑250 (MQ=255) ccgcgGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGc < 2:225845/250‑1 (MQ=255) ggcgaAAAATGTCACC‑ATCGCCATCGCGCGGAAA‑TGGCGTACTGTGCGCAATGACGATCCGATGTTCAAAGGCGTTTTAATCCGTGTCGGGGATTACTGTATCTCGGTGATGGGGCGCAGTGATGGGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATTCCGCACCGAACCGCAGTGCTACTGGCGCTTCCGTATGGGTGGTCTCTAACCGTCGCGCTAACAGTGAAATGGCCAgtt < 2:43909/248‑3 (MQ=255) ggataaatatggaccc‑atcgccatctCGCGTAAAGTTG‑GTACTCTGCTCAATGTCGGTCCGATGTTCAAAGTCGTTTAATGCCGGGACGGGGATTACTTTATCTCGGTGATGTGGTGCAGTGATGTGGTACTTAAGCAAAACGCCAATTTCCGCGTGTCGTGTCTCGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCGATCTAACCGTCTCTCCAACAGTGAAATGGTCAgct < 2:179567/237‑1 (MQ=255) aaagtGTCGCC‑ATCGCCATCGTGCGGAAA‑TTGCGTACGTTGCGCAATGGCGGTCCGATGTTCAAAGGCGGTTTAAACCGTGACGGCGATTACTTTATCTCGGTGATGGGGCGCGGTGATGTGGCACGTCAGCAAAACGCCAATTGCCTCGTGACGATTCACGCGGTGGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCTCGCCGACAGTCAAATGGCCAgctggctg < 2:85077/247‑1 (MQ=255) gTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAACGTGGATCCTATGGTCAAAGGCGTTTTAACCCGTGACGGGGAGTACGTTATCTCGGTGATGGGTCGCAGTGATGTGGCACGTAAGCAAAACTCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGTAGTGCTGCTGTCGTTGCCGTATGGGTGCTCTCTATCCGTCTTGGCAAGAGTGAAGGGGCCATCTGGGTGGagc < 2:277296/250‑1 (MQ=255) aCC‑ATCGCCAGCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGGAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAAcc < 1:354279/172‑1 (MQ=255) gccatcgcGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGGGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGGCGCGCCAGCAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGaa < 1:18444/251‑1 (MQ=255) gccatcgcGCGTAAA‑GTGCGTACTTTGCTTAATGACGAGCCGGTGTGCAAAGGCGTTTTAACCCGGGACGGGGATTACTGTAGCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCTCAGTGCGACTGGCGCTTCCGGATGGGTGCGCGCTAACCGTAGCGCCAACAGTGAAATGGCCAGCGGGCTGGAGCAGCACGAGaa < 2:18833/251‑1 (MQ=255) cgcgcgTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTACCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGt > 1:101558/1‑251 (MQ=255) gcgcgTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTc < 1:42645/251‑1 (MQ=255) gcgcgTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTc < 1:199323/251‑1 (MQ=255) gcgTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCgg > 1:24339/1‑251 (MQ=255) ggCGTACTTTGCTCAATGACGATCCGATGTGCAAAGGCGTTTTAACCCGTGACGGGGATTACTGTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCTGAGctgctg < 2:50765/250‑1 (MQ=255) aCTTTGCTCAATGACGATCCGATGGTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCGCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCGGGCTGGAGCAGCACGATAAACAGTCGGAGCTGCtgggtgg < 2:269121/251‑1 (MQ=255) aCTTTGCGCAATGACGATCCGAGGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtg < 2:130190/250‑1 (MQ=255) ttGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCGGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtggg < 1:26339/249‑1 (MQ=255) tCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCgggg > 1:201949/1‑250 (MQ=255) aTGACGATTCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGGCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGAt < 1:145085/251‑1 (MQ=255) aTGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTGATCTCGGTGATGGGGCGCAGTGATGTGGCACGTATGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGGGCGTCTGGCGCTTCCGTATGGGTGCTCTCTAGCCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGTGGCGGGTGag < 1:145086/251‑2 (MQ=255) tGACGATCCGATGTTCAAAGGCGTTTTAACACGTGACGGGGAATACCTTCTCTCGGTGCTGGGGCGAAGTGATGTGGCACGTCAGCAACACGCCAATTACCTAGTGTCGCTTCACGCTGATGCCGAACGGAACCGCAGTGCGACTGGCGCTTCCGTATGGGAGCTCTCTAACCGTCGCGCCCACAGTGACATGGCCAGATGGCTGGAGCGGCACGGGCAACAGTCGGAGCTGCTGGGTGGGGCGGGTGagg > 1:139229/1‑249 (MQ=255) cGATCCGCTGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGAAAAACGCCACTTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGATTCCGTATGGGTGCTATCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGCGGGGCGGGTGaggagc > 1:265768/1‑246 (MQ=255) gATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGcct > 2:112253/1‑192 (MQ=255) gATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCa < 1:112253/193‑1 (MQ=255) gATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGGGGTGTGCt > 1:259277/1‑251 (MQ=255) ccGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGc < 1:149123/251‑1 (MQ=255) | ACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACC‑ATCGCCATCGCGCGTAAA‑TTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGC > NC_000913/4396529‑4397014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |