Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,997	G→A	V357V (GTC→GTT)	queG ←	epoxyqueuosine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,997	0	G	A	100.0%	103.8 / NA	33	V357V (GTC→GTT)	queG	epoxyqueuosine reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/10);  total (23/10)

ATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGT  >  NC_000913/4392766‑4393243                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aTCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCAGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGCATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTCCACTCCACCAATGCACGCATTTCGtc                                                                                                                                                                                                                                     >  2:343975/1‑251 (MQ=255)     tAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGtct                                                                                                                                                                                                                                    <  1:223811/248‑1 (MQ=255)                                               ccTATTGCTAGATAAGCTAAAAATTAAGATTCCGTTTTGTCGAGTAGATCACTTGAAGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCACCTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCgaga                                                                                                                                                                                         >  2:211375/1‑249 (MQ=255)                                                       tagataAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCt                                                                                                                                                                               >  1:345820/1‑251 (MQ=255)                                                                       tAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTc                                                                                                                                                               >  1:146459/1‑251 (MQ=255)                                                                        aaGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTcc                                                                                                                                                              >  1:45564/1‑251 (MQ=255)                                                                            ttCCGTTCTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGCGCAGTGGGTGCTACCCTTTACGACTTTCCAgcg                                                                                                                                                          >  2:107270/1‑251 (MQ=255)                                                                                         agttgaTCACTTGACGATTGTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCTTCTCTCTATTTGCTGCGCAATCGCCCACGCGATGTGCTCATCGATAATTGGGTGCTCACCTTTACGGCTTTCCAGCTCTGTCAAAATCGt                                                                                                                                             <  2:394733/247‑1 (MQ=255)                                                                                                      aCGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAggg                                                                                                                                >  2:203570/1‑251 (MQ=255)                                                                                                                tCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCt                                                                                                                       >  2:409542/1‑250 (MQ=255)                                                                                                                      tgacttGCAATGCGTTTTTATTTTTATTCAAAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCAAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCCCGCTATGTGCTCATCGTGACGTTGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAACTCGTTTCATCCCCCGTGGCATTGCCTAATGCt                                                                                                                 >  2:234164/5‑250 (MQ=255)                                                                                                                       ggaTTGCAATGCGTTTTTATTTTTATTTACAATCTGTGGATGAATCAGGCGTCACGCGGTAACCCTGTTTCAATCTCCCGAACCAGACGCTGTTTGTTCGGCAATTGCACTTCAACTATGCGCGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCGATGTGCTCATCGAGAAGTGGGTGCTCGCCTGTACGTCTTTCCAGCGCGGTCAAAAGCGTTTCATCCCAAGGGGCGTTGCCTAATGCTAc                                                                                                               <  2:177958/249‑1 (MQ=255)                                                                                                                         aTTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACgg                                                                                                             >  1:163160/1‑251 (MQ=255)                                                                                                                             cAATGCGTTTTTAATTTTATTCACAAGCTGTGGATGAATCAAGCGTCCCGCGGTAACCCTTTTTCAATCACCCGAACCAGACGATGTTTTTTCGGCACTTGAACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGAGCTCATCGAGAAGCGGGTGCTCACCTTTACGACTTTCCAGAGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTCCGGCGat                                                                                                         >  2:431063/1‑251 (MQ=255)                                                                                                                                 gCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTa                                                                                                     <  1:432123/251‑1 (MQ=255)                                                                                                                                     ttttatttttatTCACACGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAACTCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGc                                                                                                  >  1:141792/1‑250 (MQ=255)                                                                                                                                               attCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGACAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGc                                                                                         >  1:128811/1‑249 (MQ=255)                                                                                                                                                  cacaAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGCTATTACGCAGCCAACGCAGGTg                                                                                    >  2:139780/1‑251 (MQ=255)                                                                                                                                                    caAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTAGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGAGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTgcc                                                                                  >  1:63032/1‑249 (MQ=255)                                                                                                                                                                      ggCGTCACGCGGTAACCCTTTTTCAAGCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAGGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACGTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGGAGCCAACGCAGGTGACCAATACGACGAATCGCCg                                                                <  1:49190/251‑1 (MQ=255)                                                                                                                                                                         gTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATc                                                             <  1:61482/251‑1 (MQ=255)                                                                                                                                                                                             cAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTaaaaa                                          <  2:129109/250‑1 (MQ=255)                                                                                                                                                                                             cAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTaaaaa                                          <  1:139780/250‑1 (MQ=255)                                                                                                                                                                                              aaTCCCCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATCTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGTGCATTGCCTAATACTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACt                                        >  2:3284/1‑251 (MQ=255)                                                                                                                                                                                              aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACt                                        <  1:203570/251‑1 (MQ=255)                                                                                                                                                                                                        aCCAGTCGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAAGATTACGAATCGCCGATCCTTCCGTGTCTTTTAAAAACTTCTCTTCGCt                              <  1:138252/251‑1 (MQ=255)                                                                                                                                                                                                          cAGACGCTGTATTTTCGGCAATTGAACTTCACCTATGAACGCATTTCGTCTCTCTATTTGATACGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTAAGACTTTACAGCGCTGTCAAAATCGTTTCATACCAAGAGGCATTGCATAATACTACGACGATTTTACGAAGCAAAAGCAGATGACCAATACAACGAAGTGCCGAACCTTCCGTGACTTTTAACAACTTCTCTTCGcac                             >  2:299970/1‑248 (MQ=255)                                                                                                                                                                                                               gCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCg                       >  1:210295/1‑251 (MQ=255)                                                                                                                                                                                                                      tttCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACt                >  1:233984/1‑251 (MQ=255)                                                                                                                                                                                                                            cAATTGCACTTCAACTATGCACGAATTTCGTCTCTCTATTTGATGCGCAATCGCCCACGCTAAGTGCTCATCGAGAAGAGGGTGCTCACCTTTACGACTTTCAAGCGCTGTCAAAATCGTTCCATCCCACGGGGCATTGCCTCATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCAAGGCGAATAACTCactgg          >  1:294412/1‑247 (MQ=255)                                                                                                                                                                                                                             aaTTGCCCTTCAACTATGCACGCATGTAGTCTCTCTATATGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGTGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCCATGGGCATTGCATAATGCTACGGCGATATTACGCAGCCAACGCAGGTGAAACATAAGACGAATCGCCTATCCTTCCGGAACTTTTAAAAACTTCTCTTCGCTCCaagctgctaactccatg           >  2:174626/1‑233 (MQ=255)                                                                                                                                                                                                                              aTTGCACTTCAACTATGCACGAATTTCGTCTCGCTATTTGCTACGCAGTCGCCAACGCTATGTGCACATCGAGCAGTGGGTGCTCCCCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCCTCCCAAGTGGCATTGCCTCACGCTACGGCGATCTTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTATTAAAAACTTCTCTTCGCTCCAGGCGaaacactcattgcgt        >  2:186131/1‑238 (MQ=255)                                                                                                                                                                                                                                    cTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGt  >  1:335337/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       ATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGT  >  NC_000913/4392766‑4393243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑