Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	42,108	G→A	intergenic (‑177/‑295)	caiT ← / → fixA	L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	42,108	0	G	A	100.0%	100.7 / NA	30	intergenic (‑177/‑295)	caiT/fixA	L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/17);  total (13/17)

CCGACGATTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAGGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCAAA  >  NC_000913/41864‑42329                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                              ccGACGATTCTTAACGGCGGACAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCCATCTTCATGTGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACCTTTAATATAATTATTATTAACCTCGTGGGCGCGTTAATGGCTAACTCATAATGGGTATTCAATGAGCTGCATTCTGTGATTGGTATAACATTTTTGTTTCGGGTGAATAGAAGGCGtt                                                                                                                                                                                                                         >  2:31166/1‑251 (MQ=255)          attaACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCTTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGGGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCTCATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGtt                                                                                                                                                                                                                 <  1:256216/250‑1 (MQ=255)               cggcggAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAAtttt                                                                                                                                                                                                           >  1:305764/1‑251 (MQ=255)                   gtgatgaaaACCTGCGGTGCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAAGTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGTCGTTTTTTCGTTAATTTTGAtt                                                                                                                                                                                                       <  2:128472/246‑1 (MQ=255)                                       tATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGct                                                                                                                                                                                   >  1:250567/1‑251 (MQ=255)                                        aTTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTTGTATCACATTTTTGTTTCGGGTGAATAGAAGGCTTTTTTTCGTTAATTTTGATTAATAATCTGTTTGTTATGct                                                                                                                                                                                   <  2:410476/250‑1 (MQ=255)                                           cccGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCtgt                                                                                                                                                                               <  1:293238/251‑1 (MQ=255)                                                tttgctattttCATGCTTCCTGAGTTAATTCCCCTGTGAAAACGAATATATATTTTGCGTTCCCGTTTGTTTTATTTGTGTTAACATTTAATATAATTATTATTAACCTAGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCTCGTTTTTGTTTCGGGTGAATAGAAGGCGTTTTTGCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTtgtg                                                                                                                                                                           <  2:336781/243‑1 (MQ=255)                                                      ctTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGCATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTaaa                                                                                                                                                                     >  2:160488/1‑250 (MQ=255)                                                                 gcATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCTTAAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACAt                                                                                                                                                            <  2:369209/247‑1 (MQ=255)                                                                                             atatTTATTTTGCGTTCCAGCTTGCTTTATTTCTATTAACATTTAATATAATTATTATTAACCTCGGGGACGCGTTAATGGCTAACTCATTATGGGTATTCAATACGCTGTATTCTGTGAATGGTATCACATTTTTGTTTCGGGTGATTAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGTGTAAAAAATAACATCTGACTTTCAATATTGTTGATCCATAAAAc                                                                                                                              >  2:76696/1‑250 (MQ=255)                                                                                               atttatttTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCACTATTGGTGATCCATAAAACAat                                                                                                                           >  2:207950/1‑251 (MQ=255)                                                                                                       tGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATAGTGaaa                                                                                                                    <  1:363252/250‑1 (MQ=255)                                                                                                       tGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAAGTTTGATTAATAATCAGTTTGTTATGCTCTGTTTTGAGTAAAAAATAACATGTTACTTTCAATATTGGTGATCCATAAAACAATATTGaaa                                                                                                                    <  1:178213/250‑1 (MQ=255)                                                                                                                                                   ttattaACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAatat                                                                                                                         >  1:214409/1‑201 (MQ=255)                                                                                                                                                   ttattaACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAatat                                                                                                                         <  2:214409/201‑1 (MQ=255)                                                                                                                                                    tattaACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTa                                                                       >  2:386056/1‑250 (MQ=255)                                                                                                                                                           cTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATAtt                                                               >  1:81583/1‑251 (MQ=255)                                                                                                                                                             cGTGGACGCGGTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTTGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATTTGACTTTCAATATTGGTGAGCCATAAAACAAGATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTg                                                              <  2:250517/250‑1 (MQ=255)                                                                                                                                                                ggACGCCTAAATGGCTAACTCATAATAAGTCTTCAATAAGCTGAATTCTGTGATCGGTATCACATTTTTGATTCGGGTGCATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTATTATGCTCTGTTGAGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAACACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGAGAGGAACTTAACAATATTGaa                                                            >  2:47062/1‑249 (MQ=255)                                                                                                                                                                                aaCTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGc                                            <  1:262919/249‑1 (MQ=255)                                                                                                                                                                                aaCTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATAGTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATGGTTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCg                                           <  2:431813/250‑1 (MQ=255)                                                                                                                                                                                   tCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGAGAGGAACTTAACAATATTGAAAGTTGGATTTTTCTGCGtgtg                                       >  2:264354/1‑251 (MQ=255)                                                                                                                                                                                     aTAATGGGTATTCCATAAGCTGTATTCTGCGATAGGAATCACATTTTTGTTTCGGGAGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTATTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGCATTATCTGCGTGTGAc                                     >  2:213268/1‑251 (MQ=255)                                                                                                                                                                                                     aaGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTg                     <  1:221778/251‑1 (MQ=255)                                                                                                                                                                                                         tGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCTATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGAtt                  >  1:260387/1‑250 (MQ=255)                                                                                                                                                                                                          gTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGAtt                  <  1:31166/249‑1 (MQ=255)                                                                                                                                                                                                            aTTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATAGTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTTGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAg              <  1:17657/251‑1 (MQ=255)                                                                                                                                                                                                                 gtgATTGGTTTCATGTTGTTGTATCTGGTGAATAGAATGCTTTTTTTCGTTAATTTTGAGTAATAATCTGGTTGTTATGCTCTGTTGTTAGTAAAAAATAGCATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGGTTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATGTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGttt           <  2:236454/249‑1 (MQ=255)                                                                                                                                                                                                                        gTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTGTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCaaa  <  1:160488/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                              CCGACGATTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAGGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCAAA  >  NC_000913/41864‑42329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑