Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3822646 =3 (0.070)49 (1.200) 45/490 0.1 92.5% coding (247/2109 nt) spoT bifunctional (p)ppGpp synthase/hydrolase SpoT
?NC_000913 = 3822651 5 (0.120)coding (252/2109 nt) spoT bifunctional (p)ppGpp synthase/hydrolase SpoT

Only 100 of 101 total aligned reads displayed.

CTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAG  >  NC_000913/3822406‑3822899
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        
cTGTTTGAAAGCATGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGATGCACGTGATGCACACGCGGGGCACACACGTTCAAGCGGTGACCCCTAAATCACGCACCCGGGAGCGGTGGCCCGCATTCTGGCCGAGATGAAACTAGACTATGAAACGCTGATGGCGGAGCTGCTGCATGACGAGCTTGCCGATACTCCCGCCACCCACCAGGAGcagg                                                                                                                                                                                                                                                      <  2:364717‑M2/250‑5 (MQ=255)
 aaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaacctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgcaacgctgatggcggcgctgctgcatgacgtgattgaagatacccccgccacctaccaggatCAGGATATGGa                                                                                                                                                                                                                                                     <  2:11866‑M1/11‑1 (MQ=255)
  gTTTGAAAGCCTGAAGCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcagga                                                                                                                                                                                                                                                     >  2:396660‑M2/1‑244 (MQ=255)
       aaaGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAAACCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGCAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACCCCCGCCACCTACCAGGATcaggatatgga                                                                                                                                                                                                                                               <  2:11866‑M2/250‑12 (MQ=255)
            caactgattcaaacctacctgccggacgaccaaatcaagcgtctgcggcaggcgtctctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgcaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTgg                                                                                                                                                                                                                                         <  2:279659‑M1/23‑1 (MQ=255)
                  cAACTGATTCAAACCTACCTGCCGGACGACCAAATCAAGCGTCTGCGGCAGGCGTCTCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGCAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttgg                                                                                                                                                                                                                                   <  2:279659‑M2/251‑24 (MQ=255)
                    tcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggagaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCg                                                                                                                                                                                                                                 <  1:59248‑M1/31‑1 (MQ=255)
                       aacctaccggccggaagaccaaatcaagcgtctgcggcaggcgtatctcgtggcacttgatgctcacgaggggcaaacacgtgcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgcggatggcggcgctgctgcatgacgtgattgaagatacgcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCg                                                                                                                                                                                                                              >  2:418410‑M1/218‑251 (MQ=255)
                          tCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGAGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcg                                                                                                                                                                                                                           <  1:59248‑M2/251‑32 (MQ=255)
                             aaCCTACCGGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTGGCACTTGATGCTCACGAGGGGCAAACACGTGCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCGGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACGCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcg                                                                                                                                                                                                                        >  2:418410‑M2/1‑217 (MQ=255)
                                gccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCt                                                                                                                                                                                                                       >  1:57792‑M1/209‑249 (MQ=255)
                                      gCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagct                                                                                                                                                                                                                 >  1:57792‑M2/1‑208 (MQ=255)
                                        accaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAgggg                                                                                                                                                                                                             <  1:246857‑M1/51‑1 (MQ=255)
                                            aatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgttattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGgtgt                                                                                                                                                                                                         >  1:12958‑M1/197‑251 (MQ=255)
                                              aCCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggg                                                                                                                                                                                                       <  1:246857‑M2/251‑52 (MQ=255)
                                                aagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaacactatatcacgcacccggtagcgggtgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGGAAAAGCGTCGCCGAGCTGGTAGGGGGGGTGTCGa                                                                                                                                                                                                      <  2:294849‑M1/58‑1 (MQ=255)
                                                  gcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAAc                                                                                                                                                                                                   >  1:294286‑M1/191‑251 (MQ=255)
                                                  aaTCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTTATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgt                                                                                                                                                                                                   >  1:12958‑M2/1‑196 (MQ=255)
                                                     gctgcggcaggcgtatctcgttgcacgtgatgctatagaggggaaaacacggtcaagatgtgaacacgatagcacgaacccggtagcggttgcctgcattctggtcgagatgaaacacgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGAGATGGAACAGCTTTTTGGTAAAAGCGTCGCTGAGCTTGGAGAGGGGGTGTCGAAACTTg                                                                                                                                                                                                >  2:423189‑M1/188‑251 (MQ=255)
                                                      aaGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACACTATATCACGCACCCGGTAGCGGGTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttgggaaaagcgtcgccgagctggtagggggggtgtcga                                                                                                                                                                                                <  2:294849‑M2/250‑59 (MQ=255)
                                                        gcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcatgctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTg                                                                                                                                                                                                >  1:185778‑M1/185‑248 (MQ=255)
                                                        gCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaac                                                                                                                                                                                             >  1:294286‑M2/1‑190 (MQ=255)
                                                           gctGCGGCAGGCGTATCTCGTTGCACGTGATGCTATAGAGGGGAAAACACGGTCAAGATGTGAACACGATAGCACGAACCCGGTAGCGGTTGCCTGCATTCTGGTCGAGATGAAACACGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggagatggaacagctttttggtaaaagcgtcgctgagcttggagagggggtgtcgaaacttg                                                                                                                                                                                          >  2:423189‑M2/2‑187 (MQ=255)
                                                              gCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATGCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttg                                                                                                                                                                                          >  1:185778‑M2/1‑184 (MQ=255)
                                                                       cgttgcacgtgatgcgcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCcgcg                                                                                                                                                                              >  2:382124‑M1/170‑251 (MQ=255)
                                                                            cacgtgatgctcactgggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattcttgccgagctgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgcttgaagatactcccgccacctaccagtatCAGGATATGGAACAGCTTTTTGGTAAACGCGTCGCCGAGCTGGTAGAGGGGGGGTCGAAACTTGATAAACTCAAGttccgcgcta                                                                                                                                                                           <  2:114550‑M1/85‑4 (MQ=255)
                                                                             cGTTGCACGTGATGCGCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcg                                                                                                                                                                        >  2:382124‑M2/1‑169 (MQ=255)
                                                                              cgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATaaga                                                                                                                                                                        <  1:237193‑M1/88‑1 (MQ=255)
                                                                                 gatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTATAGGGTGTGTCGAAACTTGATAAACTCAAGTTCCGCGATaagaaag                                                                                                                                                                     >  1:32292‑M1/160‑250 (MQ=255)
                                                                                  cACGTGATGCTCACTGGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTTGCCGAGCTGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGCTTGAAGATACTCCCGCCACCTACCAGTATcaggatatggaacagctttttggtaaacgcgtcgccgagctggtagagggggggtcgaaacttgataaactcaagttccgcgcta                                                                                                                                                                     <  2:114550‑M2/249‑86 (MQ=255)
                                                                                    cGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataaga                                                                                                                                                                  <  1:237193‑M2/250‑89 (MQ=255)
                                                                                       gATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtatagggtgtgtcgaaacttgataaactcaagttccgcgataagaaag                                                                                                                                                               >  1:32292‑M2/1‑159 (MQ=255)
                                                                                          gaggggcaaacacggtcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGc                                                                                                                                                           >  2:237193‑M1/151‑251 (MQ=255)
                                                                                                gAGGGGCAAACACGGTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggc                                                                                                                                                     >  2:237193‑M2/1‑150 (MQ=255)
                                                                                                  aacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACt                                                                                                                                                   >  1:279659‑M1/143‑251 (MQ=255)
                                                                                                    cacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCtttt                                                                                                                                                                                                                                            >  1:169126‑M1/141‑160 (MQ=255)
                                                                                                    cacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcacgccacctaccaggatCAGGATATGGAACAGCtttt                                                                                                                                                                                                                                            <  2:169126‑M1/20‑1 (MQ=255)
                                                                                                       gttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGc                                                                                                                                              <  1:28835‑M1/114‑1 (MQ=255)
                                                                                                        ttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGc                                                                                                                                              <  1:146035‑M1/114‑1 (MQ=255)
                                                                                                        aaCACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaact                                                                                                                                             >  1:279659‑M2/1‑142 (MQ=255)
                                                                                                          cacGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttt                                                                                                                                                                                                                                      >  1:169126‑M2/1‑140 (MQ=255)
                                                                                                          cacGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCACGCCACCTACCAGGATcaggatatggaacagctttt                                                                                                                                                                                                                                      <  2:169126‑M2/160‑21 (MQ=255)
                                                                                                          caagcggtgaaccctatatcacccacacggtagcggttgccttcattctgtccgagatgacactcgactatgaaacgctgatggcggcgctgctgcatgtcgtgattgaagatactccagccacctaccaggatCATGATATGGAACCGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCAATAAGAAAGAGACGCAGGCCGAAAACTTTTGCaa                                                                                                                                            >  2:1634‑M1/135‑250 (MQ=255)
                                                                                                             gTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgc                                                                                                                                        <  1:28835‑M2/251‑115 (MQ=255)
                                                                                                              ttCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgc                                                                                                                                        <  1:146035‑M2/250‑115 (MQ=255)
                                                                                                                gtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGAtt                                                                                                                                     <  2:46693‑M1/123‑1 (MQ=255)
                                                                                                                cAAGCGGTGAACCCTATATCACCCACACGGTAGCGGTTGCCTTCATTCTGTCCGAGATGACACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGTCGTGATTGAAGATACTCCAGCCACCTACCAGGATcatgatatggaaccgctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcaataagaaagagacgcaggccgaaaacttttgcaa                                                                                                                                      >  2:1634‑M2/1‑134 (MQ=255)
                                                                                                                  gatccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTAt                                                                                                                                   <  1:352946‑M1/125‑1 (MQ=255)
                                                                                                                        gatccctatATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattat                                                                                                                             <  1:352946‑M2/248‑126 (MQ=255)
                                                                                                                        gAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGCGCTGGTAGAGGGGGTGTCGACACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCACGATGATTATGGCGAt                                                                                                                             >  2:138073/1‑251 (MQ=255)
                                                                                                                            tcacgcacccggtagcggttgcctgcattctggccgagatgaacctcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGCGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCACGATGATTATGGCGATGGTg                                                                                                                         <  1:22023‑M1/135‑1 (MQ=255)
                                                                                                                                  tCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAACCTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgcgctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcacgatgattatggcgatggtg                                                                                                                   <  1:22023‑M2/251‑136 (MQ=255)
                                                                                                                                        tagcggttgactgcattctggcagagatgaaactcgactatgaaacgctgatggcggcactgctgcatgacgtgattgacgatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGCGCTGGTAGCGGGGGTGTCGAAACTTGATAAACTCAAGTTACGCGATAAGAAAGAGGCGCAGGCAGAAAACTATCGCAAGATGATTATGGCGCTGGTGCAGGAAATCcgc                                                                                                             <  2:247778‑M1/147‑1 (MQ=255)
                                                                                                                                         agcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCcgcg                                                                                                            >  1:46693‑M1/104‑251 (MQ=255)
                                                                                                                                            ggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGGTATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCa                                                                                                         >  1:115181‑M1/101‑251 (MQ=255)
                                                                                                                                            ggttgcctgcattctggccgagatgaaactcgactatgaaacgctgagggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGTGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAGGAGGATTATGGCGATGGTGCAGGATATCCTCGTCa                                                                                                         >  1:248976‑M1/101‑251 (MQ=255)
                                                                                                                                              ttgcctgcattctggccgagatgaaactcgactatgcaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggctCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCAGCGATAAGAAAGAGGCGCAGCCCGAAAACTTTCGCACGATGATTATGGCGATGGTGCAGGATATCCGcgtcctc                                                                                                       <  2:387020‑M1/153‑4 (MQ=255)
                                                                                                                                              tAGCGGTTGACTGCATTCTGGCAGAGATGAAACTCGACTATGAAACGCTGATGGCGGCACTGCTGCATGACGTGATTGACGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgcgctggtagcgggggtgtcgaaacttgataaactcaagttacgcgataagaaagaggcgcaggcagaaaactatcgcaagatgattatggcgctggtgcaggaaatccgc                                                                                                       <  2:247778‑M2/251‑148 (MQ=255)
                                                                                                                                               aGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcg                                                                                                      >  1:46693‑M2/1‑103 (MQ=255)
                                                                                                                                                gcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggagCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGTCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCt                                                                                                     >  1:55517‑M1/97‑251 (MQ=255)
                                                                                                                                                 cctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAgat                                                                                                                                         <  1:220486‑M1/119‑1 (MQ=255)
                                                                                                                                                 cctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTGTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTGTCGCAAgat                                                                                                                                         >  2:220486‑M1/96‑214 (MQ=255)
                                                                                                                                                  ggTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcagggtatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtca                                                                                                   >  1:115181‑M2/1‑100 (MQ=255)
                                                                                                                                                  ggTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGAGGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagaggtggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaggaggattatggcgatggtgcaggatatcctcgtca                                                                                                   >  1:248976‑M2/1‑100 (MQ=255)
                                                                                                                                                    ttGCCTGCATTCTGGCCGAGATGAAACTCGACTATGCAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGCTcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttcagcgataagaaagaggcgcagcccgaaaactttcgcacgatgattatggcgatggtgcaggatatccgcgtcctc                                                                                                 <  2:387020‑M2/251‑154 (MQ=255)
                                                                                                                                                      gCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGAGcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcagtccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcct                                                                                               >  1:55517‑M2/1‑96 (MQ=255)
                                                                                                                                                       ccTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagat                                                                                                                                   <  1:220486‑M2/214‑120 (MQ=255)
                                                                                                                                                       ccTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttgtggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactgtcgcaagat                                                                                                                                   >  2:220486‑M2/1‑95 (MQ=255)
                                                                                                                                                        tctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAAc                                                                                             <  1:152445‑M1/163‑1 (MQ=255)
                                                                                                                                                         ctggccgagatgaaactcgactatgaaacgctgatggcagcgatgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAACAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAACCTCAAGTTCCGCGCTAAGAAAGAGCCGCAGGACGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACt                                                                                            <  1:273990‑M1/164‑1 (MQ=255)
                                                                                                                                                              tCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaac                                                                                       <  1:152445‑M2/251‑164 (MQ=255)
                                                                                                                                                              cgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCg                                                                                       >  1:97194‑M1/83‑251 (MQ=255)
                                                                                                                                                               cTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCAGCGATGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaacagcgtcgccgagctggtagagggggtgtcgaaacttgataacctcaagttccgcgctaagaaagagccgcaggacgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaact                                                                                      <  1:273990‑M2/251‑165 (MQ=255)
                                                                                                                                                                  atgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCg                                                                                   <  1:5085‑M1/173‑1 (MQ=255)
                                                                                                                                                                    cGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccg                                                                                 >  1:97194‑M2/1‑82 (MQ=255)
                                                                                                                                                                        aTGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccg                                                                             <  1:5085‑M2/251‑174 (MQ=255)
                                                                                                                                                                           gacgaggaaacgcggattgcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCGTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACGCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACATGCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGAACGTACCCacaa                                                                          >  2:228572‑M1/70‑251 (MQ=255)
                                                                                                                                                                                 gacgaggaaacgcGGATTGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagcgttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaacgcaagttccgcgataagaaagaggcgcaggccgaaaacatgcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaacgtacccacaa                                                                    >  2:228572‑M2/7‑69 (MQ=255)
                                                                                                                                                                                                        gcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGGTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCTCATTATGATTATGGCGATGGTGCAGGATATCCGCGGCATCCTCATCAAACTGGCCGACCGTACCCACATCATTCGCACGCTGGGCTCACTTCGccc                                               >  2:21423‑M1/41‑249 (MQ=255)
                                                                                                                                                                                                           tgccgtggttgaagatactcccgccccctaccaggatCAGGATATGGAACAGCATTTTGGTACAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCCCGCTGGGCTCACTTCGCCCGGAc                                           <  2:327379‑M1/213‑1 (MQ=255)
                                                                                                                                                                                                              gcATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaaggtccgcgataagaaagaggcgcaggccgaaaactttctcattatgattatggcgatggtgcaggatatccgcggcatcctcatcaaactggccgaccgtacccacatcattcgcacgctgggctcacttcgccc                                         >  2:21423‑M2/1‑40 (MQ=255)
                                                                                                                                                                                                                 tgccgtgGTTGAAGATACTCCCGCCCCCTACCAGGATcaggatatggaacagcattttggtacaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcccgctgggctcacttcgcccggac                                     <  2:327379‑M2/247‑214 (MQ=255)
                                                                                                                                                                                                                  attgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTcg                                   >  1:294849‑M1/31‑251 (MQ=255)
                                                                                                                                                                                                                        aTTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcg                             >  1:294849‑M2/1‑30 (MQ=255)
                                                                                                                                                                                                                         atactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATc                            <  1:15197‑M1/228‑1 (MQ=255)
                                                                                                                                                                                                                              cccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCTCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGccc                        >  1:247778‑M1/19‑250 (MQ=255)
                                                                                                                                                                                                                              cccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCg                       >  1:11866‑M1/19‑251 (MQ=255)
                                                                                                                                                                                                                               aTACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatc                      <  1:15197‑M2/251‑229 (MQ=255)
                                                                                                                                                                                                                                   cccctacaaggatCAGGATATGGAACAGTTTTTTTGTAAGAGCGTCGCCGAGCTGGTAGAGGGGTTGTCGAAACTTGATAACCTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGACAACTTTCGCAAGATGATCATGGCGATGGTGCAGGATATCCGCGTCATCCTCAGCAAACTTTCCGACCGTCCCCACACCATGCGCACGCTGGGCTCGCTTCGCCCGGACAAACGTCGCAGCAGCGCCCGTGaaa                  >  2:100708‑M1/14‑251 (MQ=255)
                                                                                                                                                                                                                                    cccGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgctcacgctgggctcacttcgcccggacaaacgtcgccgcatcgccc                  >  1:247778‑M2/1‑18 (MQ=255)
                                                                                                                                                                                                                                    cccGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccg                 >  1:11866‑M2/1‑18 (MQ=255)
                                                                                                                                                                                                                                         cccctaCAAGGATcaggatatggaacagtttttttgtaagagcgtcgccgagctggtagaggggttgtcgaaacttgataacctcaagttccgcgataagaaagaggcgcaggccgacaactttcgcaagatgatcatggcgatggtgcaggatatccgcgtcatcctcagcaaactttccgaccgtccccacaccatgcgcacgctgggctcgcttcgcccggacaaacgtcgcagcagcgcccgtgaaa            >  2:100708‑M2/3‑13 (MQ=255)
                                                                                                                                                                                                                                         ccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCg            >  1:300289‑M1/8‑251 (MQ=255)
                                                                                                                                                                                                                                           aggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGTCCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGa           <  1:91015‑M1/245‑1 (MQ=255)
                                                                                                                                                                                                                                               ccAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcg      >  1:300289‑M2/1‑7 (MQ=255)
                                                                                                                                                                                                                                                cAGGATATGGAGCAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAAttt      <  2:193357/250‑1 (MQ=255)
                                                                                                                                                                                                                                                cAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAAttt      <  1:99718/250‑1 (MQ=255)
                                                                                                                                                                                                                                                cAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAAttt      >  1:137701/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 aggatcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgtccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcga     <  1:91015‑M2/250‑246 (MQ=255)
                                                                                                                                                                                                                                                   gATATTGAACAGATTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAATCTTTCGCAAGATGATTATTGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATTCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAg  <  1:317067/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        
CTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAG  >  NC_000913/3822406‑3822899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.