Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	663,262	IS2 (–) +5 bp	coding (698‑702/1212 nt)	dacA ←	D‑alanyl‑D‑alanine carboxypeptidase DacA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	663262 =	0 (0.000)	24 (0.590)	21/490	0.7	100%	coding (702/1212 nt)	dacA	D‑alanyl‑D‑alanine carboxypeptidase DacA
	?	NC_000913	= 1469240	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 663266	0 (0.000)	32 (0.780)	29/490	0.4	100%	coding (698/1212 nt)	dacA	D‑alanyl‑D‑alanine carboxypeptidase DacA
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

AACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCAC  >  NC_000913/1467666‑1468157                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        aaCGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATCGTTATCCCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGAAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGATTTGAACATCATGGGACAAGAACCCTGGAttt                                                                                                                                                                                                                                                   >  1:276899/1‑251 (MQ=255)                  gTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGa                                                                                                                                                                                                                                  <  1:386804/251‑1 (MQ=255)                               tCCTTGCGCACAGGATTTAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCa                                                                                                                                                                                                                      <  2:293880/250‑1 (MQ=255)                                        acaGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTGACCCAt                                                                                                                                                                                                            <  1:117863/251‑1 (MQ=255)                                          aGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATATGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCAAGACATCTGTTATCCCTTAACCCAtt                                                                                                                                                                                                           >  1:249872/1‑250 (MQ=255)                                           ggATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTAc                                                                                                                                                                                                         >  1:197344/1‑251 (MQ=255)                                            gATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTAc                                                                                                                                                                                                         >  1:250557/1‑250 (MQ=255)                                                 aGTTATACCAGCGTTTTTGTCGTTAGTGGTCAGACGTAGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGccc                                                                                                                                                                                                   >  2:276670/1‑251 (MQ=255)                                                                      gTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTg                                                                                                                                                                              >  2:153938/1‑251 (MQ=255)                                                                          gtggtgAGGAGTCGGTATCTCTAAATGGAGATACGACAATCAGCGGAGAAATCCCTTTGTGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCATTACTGAAAATAGGCAGTGGCGCTACGCTAACAATGCAGGATATTGCCAGATTTGAACATCATGGGACAAGAAACCTGGATGTGCCCCTATATTTACAGACATCTGATATCACTTAACCCATTACAATCCCGCTCCAGCAGAGATTCCCGTGgcg                                                                                                                                                                           >  2:56514/1‑250 (MQ=255)                                                                             gtgAGTCGTCGGTATAGCTGAATGGAGATACGACAATCAGCGGAGAATTCTCTCGGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcgagcg                                                                                                                                                                       <  2:339676/251‑1 (MQ=255)                                                                                      cgggatatCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGGTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCa                                                                                                                                                               <  2:354497/246‑1 (MQ=255)                                                                                            atctgacgggAGATACGAACATCAGAGGAGCATTACATCTGGCGATTGCAGGGGTTATTCGGGTACAGGATAAAGCTTTTCTGGCACTTGGCAGTGGCGCTACACTAACAATGCAGGATATTGACAGTTTTGAACATCCAGGGACAAGAACACTGGATTTGCCACTATATTTCCAGACATATGTTATCACTTAACCCATTACATGCACGCTGCCGCAGATATTCCCGTGGCGTGCGATAAACCAGCGCAcc                                                                                                                                                        >  2:165595/9‑250 (MQ=255)                                                                                                gAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGc                                                                                                                                                    <  1:205923/251‑1 (MQ=255)                                                                                                  aTGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCgg                                                                                                                                                  >  1:234839/1‑251 (MQ=255)                                                                                                          aCGAAAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGGTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTGAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt                                                                                                                                          <  2:197344/251‑1 (MQ=255)                                                                                                               aaTCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGtt                                                                                                                                      >  1:40382/1‑250 (MQ=255)                                                                                                               aaTCAGCGGAGAATTCACTCTGGGGATTGCCGGGGTTATTCGGGTACAGGATAAAGATTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATAACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGAAGCCATTCGtt                                                                                                                                      >  1:44757/1‑250 (MQ=255)                                                                                                                      ggAGAATTCCCTCTGGGGTTTGCCGGGGTGATTCGGATACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTTCGCTAACAATGCAGGATCTTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGCTATTCCCTAGGCGAGCGATACCCCAGCGCACTATGCGGATGCCATTCGTTATAATGCt                                                                                                                              >  2:409547/1‑251 (MQ=25)                                                                                                                                                ggTTATTCGGGGACAGGATAAAGCTTTGCTTGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCTAGCGATAACCCAGCGCACTATGCGGATTCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTtgct                                                                                                    <  2:433739/251‑1 (MQ=255)                                                                                                                                                         ggTACAGGATAAAGCTTTGCTGGAAATTGGCAGAGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGAGACAAGAACCCTGGATTTGACCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAAc                                                                                           >  1:354335/1‑251 (MQ=255)                                                                                                                                                         ggTACAGGATAAAGAGTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATAATGGGACAAGAACCCTGGATTTACCCATATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCACATGTCATTAGTTATCATGATCGAAAGCCTCTGCAAGGTTATTTTGTGCCGtttat                                                                                           >  2:238252/1‑248 (MQ=21)                                                                                                                                                               ggATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCt                                                                                     >  2:144077/1‑251 (MQ=255)                                                                                                                                                                           gCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATg                                                                         <  1:262448/251‑1 (MQ=255)                                                                                                                                                                                 aaTTGGCAGTGGCGCTACGCTAACCATGCAGGCTATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCAGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGATCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTg                                                                   >  1:134398/1‑251 (MQ=255)                                                                                                                                                                                  aTTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTg                                                                   >  1:403211/1‑250 (MQ=255)                                                                                                                                                                                   ttGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGATTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGAt                                                                 >  1:140078/1‑251 (MQ=255)                                                                                                                                                                                          tGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGACCCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTCTGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCCAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATCATGATGTAGTc                                                           >  2:344676/1‑250 (MQ=255)                                                                                                                                                                                           ggCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCa                                                          <  1:276670/250‑1 (MQ=255)                                                                                                                                                                                                         aaTGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGACCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCACGCTGCCGCAGATATTCCCGTGGCGAGAGATAACCCAGAGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTc                                           >  1:145573/1‑251 (MQ=255)                                                                                                                                                                                                            gCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTTGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCAc                                         <  1:332375/250‑1 (MQ=255)                                                                                                                                                                                                               ggATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGa                                       >  1:30958/1‑249 (MQ=255)                                                                                                                                                                                                                              tGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCg                      >  1:359838/1‑251 (MQ=39)                                                                                                                                                                                                                               gAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCg                      <  1:153938/250‑1 (MQ=39)                                                                                                                                                                                                                               gAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGt                     >  1:427030/1‑251 (MQ=39)                                                                                                                                                                                                                                     catGGGACAAGAACCCTGGATTTGCCCCTATCTTTCCCGGCATCTGTTATCACTTAAACCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGTTAACACAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGCAGCTCTCTGCTATTCCGTTActct               >  1:218623/1‑251 (MQ=11)                                                                                                                                                                                                                                          gACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACACGCCCGCTGCCGCAGATATTCCCGTGGAGAGCGATAACCCAGAGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGAAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCAGTTACTCTCCgg           >  2:250129/1‑250 (MQ=255)                                                                                                                                                                                                                                                  cccTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTTGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCGTTATCGTTTTCACGAAGCTCTCTGCTATGCCGTTACTCTCCGTACTCCGCAc  <  2:250557/251‑1 (MQ=2)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        AACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCAC  >  NC_000913/1467666‑1468157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.