Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,906,143	IS5 (+) +4 bp	coding (433‑436/873 nt)	ygcG →	TPM domain‑containing protein YgcG

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	32 (0.780)	30/490	0.4	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	2906143 =	0 (0.000)					coding (433/873 nt)	ygcG	TPM domain‑containing protein YgcG
*	?	NC_000913	= 2906146	0 (0.000)	29 (0.710)	28/490	0.4	100%	coding (436/873 nt)	ygcG	TPM domain‑containing protein YgcG
	?	NC_000913	3130146 =	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AATTTGATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAA  >  NC_000913/2288676‑2289160                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   aaTTTGATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTTATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCAGCAACTTTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTg                                                                                                                                                                                                                                             <  2:376649/251‑1 (MQ=255)          aCCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATAATCAACAGTTGATGCCCATGCTCTGGGTGAATATAAGGAAGGTGCGAATAAGc                                                                                                                                                                                                                                    >  1:134087/1‑251 (MQ=255)                   tAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCGTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAAt                                                                                                                                                                                                                            <  2:319927/250‑1 (MQ=255)                                        gcCATTTATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGtcat                                                                                                                                                                                                     >  1:123314/1‑251 (MQ=255)                                                  tCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGGTTTCTTTTTTCCGGACAAAATCATCAACTGACTCACCATACTCAACAGTTAATGCAAATGCTATTGGTGAATATAAGGCAGGTGCGAAAAAACGGGGAAATTTTTCTAGGCTAGCT‑CAGGcatttcatttttt                                                                                                                                                                                           >  1:179594/1‑248 (MQ=255)                                                     aTGTACTCATCCCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTAGATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCAt                                                                                                                                                                                        <  2:109931/251‑1 (MQ=255)                                                               aCCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGAGcc                                                                                                                                                                               <  1:420627/250‑1 (MQ=255)                                                                    ccGTTAATTTCAGTATTATTACTTACTCCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGAGCCGAtt                                                                                                                                                                           <  2:117709/249‑1 (MQ=255)                                                                                                  aaTTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCCGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCCTACTCAACCGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTTATGTTTGAGCCGATTTTTTCTCCCGTAAAATACTTGAATCACCCTAt                                                                                                                                           >  2:366190/1‑251 (MQ=37)                                                                                                                                      ttCGTACTATTGGCGGTCCCCTAAACCAGCTGGATATCTTTTTCCAGGACCACACCATCAACTGTCTCACCATACTCAACAATTGATGCCCATGCTATGGGTGAATATCAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCCTTTCTTTATGTTTTAGCCGATTTTTTCTCCCGTAAATTCCTTAAAACAGCCTATTTTGACCGTTTCTTCGCCATTTTATGCGTTATcccc                                                                                                       >  1:112580/1‑251 (MQ=21)                                                                                                                                          tattatTGGCGGTCCCATAAACCAGCTGGGAATCTTTTTCCTGGACAACACCATACACTGTCTCACCATACTCAACAGTTGATGCCAAAGCTATGGGGGAATATAAGGAAGGTGCGAATAAGCGGGGGAATTCTTCTCTGGTGACT‑CAGGCATTTCATTTTTTCATGTTTGAGCCGCTTTTTTCTCCCGTAAATGACTTGAAACAGCCTATTTAAACCGTTTTTTTGCCATTTAAGGGGTTTTCCCCAGtt                                                                                                   >  2:80170/1‑251 (MQ=255)                                                                                                                                                tGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCATCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAg                                                                                              <  2:134340/250‑1 (MQ=255)                                                                                                                                                    ggTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGACATTTAAGGCGTTATCCCCAGTTTTTAGTgcgg                                                                                         >  2:5398/1‑248 (MQ=255)                                                                                                                                                    ggTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACAACCTCAACTGTATCACCATACTCAACAGTTGCTCCCAACGCTCTGGGTGAATATAAGGCAGGTGCGAATAAGCGAGGAAATTCTTCTCGGCTGACT‑CCGTCATTTCATTTCTTCATGTTTGCGCCGATTTTTTATCCCGTACATGCCTTGATTCAGCCTATTTAGACCGTTTCTTCGCCTTTTAAGGCGTTATCCCCAGTTTTTAGTg                                                                                            >  2:195340/1‑248 (MQ=255)                                                                                                                                                      tCCCATAAACCAGCTGGATATCTTTTTCCACGAACACAACATCCACTGTCTAACCATACTCAACAGTTGCTGCCAATGCAATGGGTGAATCTAAGGAAGGTGCGAACAAGCGGGGAAATTCTTCTCAGCTGACTACA‑TCCTTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGAAATTTACGGCGTTATCCCCAGTTTTTAgcg                                                                                            >  1:35726/1‑244 (MQ=17)                                                                                                                                                             aaCCAGCTGGATATCTTTCTCCAGGACAACACCATCAACTGGCTCACCATACTCAACAGTTGAGGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCAGTTCATTTCTGCATGTTTGAGCCGATTTTTGCTCCCGGAAATGCCTTGAATCAGCCTATTTAGACCGTTTCCTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCa                                                                                <  2:153216/251‑1 (MQ=18)                                                                                                                                                                                    ggACAACACCATCAACTGTCTCGCCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACG‑CAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTTCGccc                                                         <  2:245532/251‑1 (MQ=255)                                                                                                                                                                                               tCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTg                                              >  1:149702/1‑251 (MQ=255)                                                                                                                                                                                                 aaCTGTTTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATCTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGCGCCGATTTTTTCTCCCGTAAATGCCTTGCATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATATCTCACACTGACGTATCATTTGGTCCACCCGAAACCGGTTGGc                                            >  1:120360/1‑251 (MQ=17)                                                                                                                                                                                                     gTCTCACCATACTCAACAGTTGATGCCAATGCTAGGGGTGAATATAAGGAATGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTGCATTTCTTCATGTTTGAGCCGATGTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGCCCGTTTCTTCGTCATGTAAGTCGTTATCTCCAGTTTTTAGTGAGAGCTCTCCCACTGACGTATCATTTGGTCCTCCCGAAACATGTTGGCCa                                          <  2:239397/249‑1 (MQ=17)                                                                                                                                                                                                                cTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACAt                             >  1:111702/1‑251 (MQ=255)                                                                                                                                                                                                                  cAACAGTTGATGCCAATGCTATGGGGGAATATAAGGAAGGTGCGAATAAGCGGGGCAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCg                           >  2:372273/1‑251 (MQ=255)                                                                                                                                                                                                                    aCAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGcc                         >  2:320903/1‑251 (MQ=255)                                                                                                                                                                                                                     cAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATGTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCa                        <  2:134087/251‑1 (MQ=255)                                                                                                                                                                                                                                            gAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCaa  <  1:427574/250‑1 (MQ=32)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   AATTTGATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACT‑CAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAA  >  NC_000913/2288676‑2289160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.