Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,737,689	IS150 (+) +3 bp	intergenic (+43/‑54)	pheL → / → pheA	phe operon leader peptide/fused chorismate mutase/prephenate dehydratase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2737689 =	0 (0.000)	12 (0.300)	12/488	1.4	100%	intergenic (+43/‑56)	pheL/pheA	phe operon leader peptide/fused chorismate mutase/prephenate dehydratase
	?	NC_000913	= 3722075	NA (NA)					noncoding (1443/1443 nt)	IS150	repeat region
*	?	NC_000913	= 2737691	0 (0.000)	42 (1.040)	38/486	0.2	100%	intergenic (+45/‑54)	pheL/pheA	phe operon leader peptide/fused chorismate mutase/prephenate dehydratase
	?	NC_000913	3720633 =	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

ATATCACGTACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2737890‑2737689 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCAAGATAAAAACACTCCGACTTTAAGGTTCCAAAGAAACACTCCACCACAGCATTATCCAGACAATTGCCTTTTCTGGAC  <  NC_000913/3722075‑3721830                                                                                                                                                                                                                                                                                                                                                                                                                                                                  ATATCACGTACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCT                                                                                                                                                                                                       >  1:398365/1‑251          ACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGACAGCACGTGCCGCTCTGCCAGTAACGCTAATAATTTTTCAACCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTCTTATCAATAAAAAAGGCCCCCCGCTTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGAT                                                                                                                                                                                              >  1:219648/1‑251                                                GACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCTGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATT                                                                                                                                                        >  1:11682/1‑251                                                  CGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCT                                                                                                                                                      <  1:179048/251‑1                                                                        CAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAAT                                                                                                                                 >  1:47066/1‑250                                                                                  AATAATTTTTCATCCAGCGCGCTGATTTTCTCACGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGTTTTGGGAGGCCTTATTGTGTACTGACCCCTAAAAGTTTGGCAGTTAAACACGAGGCATATAGGTCTGATTCCGATAGTCAATTGGAGTCAGTCCTTTTAATTTTTGGCGAAGTGTTCTGCTGTTGTAGGATTCAATATATTCCGTAA                                                                                                                      <  1:405863/251‑1                                                                                                                                    GTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAAAACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCAAGATAAAAACAC                                                                    >  1:199429/1‑251                                                                                                                                         CCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCAAGATAAAAACACTCCG                                                                <  1:360341/250‑1                                                                                                                                                                        ATAAAAAAGGCCCGCCGATTTGGGCGGCCTTATTGTGTACTGACCCCCAAAAGTTGGACAGTTAACCACGAGGCATATAGCGCTGATTCCGCTATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCCAGATAACAACCCTCCGACTTTAAGGTTCCAAAGAAACACTCCACCAC                                 >  2:219125/1‑250                                                                                                                                                                                         ATTTGGGAGGCCTTATTGTGTACTGTCCACAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATGCCGTAACAGCATCCTACAGTTCGCTTATATTACTGAACTCATCAAGATAAAAACACCCCGACTTTAAGGTTCCAAAGAAACACTCCACCACATCATTATCCAGACAATT               <  1:219125/251‑1                                                                                                                                                                                             GGGAGGCCTTAGTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCAAGATAAAAACACTCCGACTTTAAGGTGCCAAAGAAACACTCCACCACAGCATTATCCAGACAATTGCC            <  2:87654/250‑1                                                                                                                                                                                                       ATTGGGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTATTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTGATATTACTGAACTCATCAAGATAAAAACACTCCGACTTTAAGGTTCCAAAGAAACACTCCACCACAGCATTATCCAGACAATTGCCTTTTTTGGAC  <  2:30567/250‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                  ATATCACGTACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2737890‑2737689 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCAAGATAAAAACACTCCGACTTTAAGGTTCCAAAGAAACACTCCACCACAGCATTATCCAGACAATTGCCTTTTCTGGAC  <  NC_000913/3722075‑3721830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.