Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 399,639 +A coding (47/213 nt) yaiZ → DUF2754 domain‑containing protein YaiZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913399,6391.A96.5% 80.3 / ‑3.1 29coding (47/213 nt)yaiZDUF2754 domain‑containing protein YaiZ
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base A (11/17);  total (11/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01

AGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTT‑CGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCG  >  NC_000913/399391‑399875
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            
aGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACCCCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑Gttac                                                                                                                                                                                                                                             <  1:320275/251‑3 (MQ=255)
 gTCTGACAGGTCATTTAGATTGTAAATATCAGAATCATGGTCAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑Gttacg                                                                                                                                                                                                                                            >  1:149726/1‑248 (MQ=255)
   cTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑Gttacgcc                                                                                                                                                                                                                                          >  2:407732/1‑246 (MQ=255)
             aTTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTta                                                                                                                                                                                                                                <  1:139521/251‑1 (MQ=255)
                      gTCAATACCAGAATCAGGGTAAATTGATGTGGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCCCAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACCCCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑TTTACGCCATTGGCCTTATattcattc                                                                                                                                                                                                                       <  2:70641/251‑1 (MQ=255)
                         aaTATCAGAATCATGGTAAATTGATGTGGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAAGGGCAACCTTAAGTTAAATTTATTAAAAATGACGAAAATGTTGGCTACACCACAGACGGTTGTTGCAGAATATGCAAGGAGGTTGTTT‑TTCTGTAACGGTGCTGCCATGAACCTGCCTGGAAAAGTCCGCCGCGACTGGCACTAGTATGCTGTA‑CGCCATTGGCCTTATATTTATTCTTa                                                                                                                                                                                                                    <  2:26060/251‑1 (MQ=255)
                                     aTGGTCAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACAACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGtggtgg                                                                                                                                                                                                        >  1:350338/1‑251 (MQ=255)
                                                      ggAATATTCCCTAAGCTGGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAAGTTATTAACAATTACGAAAATTTTTTCTTCACCACAGACGGTTGTTGAAGAATATTCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATTAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTAGGGCATTGGCCTTATATGCATTCTTAATGGCGTGGTGGGGTGACGGGGATTTGaa                                                                                                                                                                                       <  2:126592/251‑1 (MQ=255)
                                                                                             aaaTGGCCACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCCCTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGGAGCAAAAGGTTGGCAGACCTATGCCGTCGGTTTGGGGACg                                                                                                                                                >  2:118935/1‑251 (MQ=255)
                                                                                                             ttAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTct                                                                                                                                >  1:327757/1‑251 (MQ=255)
                                                                                                                 aTTTATAAACAAGTACGAAAATGTTGTCTACACCAAAGACGGTTGTGGCAGAATAGGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTctggct                                                                                                                            <  2:411974/251‑1 (MQ=255)
                                                                                                                      ttaACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCgg                                                                                                                       >  1:51800/1‑251 (MQ=255)
                                                                                                                             ttACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGat                                                                                                                >  1:19290/1‑251 (MQ=255)
                                                                                                                                       gttgtCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAATCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATtcgtcg                                                                                                       <  1:264733/250‑1 (MQ=255)
                                                                                                                                                         ggTTGCTGCAGTATATGCAAGGATGTTGTTT‑TTCGTTAACGGCGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGATTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGTTTTGAAGCAACAGGTTGGCAGGCCTATGACTGCGGTCTGGGGCCGGGGGTGATTAGTTTCTGGCTGTCGGGGTTGATTATtctgtgtcgaggttaagaatatgc                                                                                     >  2:234354/1‑228 (MQ=255)
                                                                                                                                                                  aGAATTTGAAAGGAT‑TTGTTTGTTTGTTAACGGAGCTACCATGAACTTGCCTTGAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGTTTACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCTAGGAAGAAACGGAAAACGCCCaa                                                                           <  2:85576/251‑1 (MQ=255)
                                                                                                                                                                       atGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAg                                                                       <  1:7653/250‑1 (MQ=255)
                                                                                                                                                                          cAAGGTTGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAata                                                                   <  1:229489/251‑1 (MQ=255)
                                                                                                                                                                           aaGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAAACTGCCTCTAAAAATCCGCCGCGACTGGCACAACTATGC‑GTTACGACATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGCGTACTGGGATTTGAAGAAAAAGGTTGGCAGCCCCATGCCGTCGGTATGGTGACGGGGGAGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGCTGAACAAACTGACAACGCCCAATAAGAata                                                                   >  1:145361/1‑250 (MQ=255)
                                                                                                                                                                              gATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGACATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGCCTGGTGCCGTGGGTGATTAGTTTCTGGCTGGCGGGGGTGGTTATTCGTCGTCGCGGTTAAGAAACTGAAAAAGCCCCATCAGAAaattat                                                               >  2:80670/1‑251 (MQ=255)
                                                                                                                                                                                   tgttT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCggg                                                           <  1:407732/250‑1 (MQ=255)
                                                                                                                                                                                              tAACGGAGCTGCCATGAATCTGCCTGGAAAAATCCGCCGTTACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCTGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGGAAAAata                                                 <  1:123040/250‑1 (MQ=255)
                                                                                                                                                                                               aaCGGACCTCCCTTGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGTGCGTTAAAATAtt                                               <  2:154747/251‑1 (MQ=255)
                                                                                                                                                                                                  gtaGCTGCCATGAAACTGCCTGTAAAAATCCGCCGTGAATGGCACTACTATGC‑GTTACGCCATTGGCCTTATATACATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTGGATTATTCGTCGACGCGATGAAGAAACTGAAAACGCCCAATAAGAATATGATCGGGCGTTAAAATATTACa                                            <  2:117605/249‑1 (MQ=255)
                                                                                                                                                                                                         ccATGAATCGTCCGGTAAAAATCCGCCTTGAATGGCACTACTAGGC‑GTTACGCCATTGGCCGTATATTCATTCTTAATGGCGTGTTGGTGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCTTCGGTCTGGTGACGTGGGTGATTAGTTTCTTGCTGTCGGGGTTTATTATTCGTCTTCGCGATGAAGAAACTTAAAACGCACAATAAGAATTTTATCTGGCGTTAAAATATTACAATGTGGt                                     <  2:291817/251‑1 (MQ=255)
                                                                                                                                                                                                             gAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCTTTAAAATATTACATTGTGGTTTTc                                 <  2:19290/251‑1 (MQ=255)
                                                                                                                                                                                                                     cTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGCTGTAGAAACTGAAAACGACCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATTCGtt                         >  1:269909/1‑251 (MQ=255)
                                                                                                                                                                                                                       gTAAAAATCCGCCGTGACTGGCGCTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTTCTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTGTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTAGTCGTCGTCGCGAGGAAGAAACTGAAAACGCCCAATAAGAACATTAGCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGtt                         <  1:244154/249‑1 (MQ=255)
                                                                                                                                                                                                                           aaaTCCGCCGTGACTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGATGGCGGGGTTGATTTTTCGGTGACGCGATGAAGCAACTGAAAACGCCCAATACGAATATTATCGGGGGGTCAAATATTACAtttggggttttaattggttatacgc                   >  2:383123/1‑228 (MQ=255)
                                                                                                                                                                                                                             aTCCGCCGTGACTGGCACTACTATGC‑GTTACGACAGTGGCCTTATATTTATTCTTAAGGGCGTTGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGTCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGAGTATTCGTCGTCGCGAGGAAGAAACTGAAAACGCCTAATAAGAATATTAGCGGGCGTTAAGAGATTACATGGTGGTGTTCAATGCGTTCTCCGCAg                 <  2:392733/251‑1 (MQ=255)
                                                                                                                                                                                                                                        cTGGCACTACTATGC‑GTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGAT‑ATTCGTCGTCGCGATGGAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACCTTGTGGTTTTAAATGCGTTATCCCCAGCGTGACGCTccc     >  2:75986/1‑250 (MQ=255)
                                                                                                                                                                                                                                            cACTACTATGC‑GTTACGCCATTGTCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACGGAAAACGCCCAATAAGAATATTAGCGGGCGTTAAAATATTGCATTGTGGTTTTCAATGCGGTATCCGCGGCGTGACGCTCCAgcg  <  2:149726/251‑1 (MQ=255)
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            
AGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTT‑TTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGC‑GTT‑CGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCG  >  NC_000913/399391‑399875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑