Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,282,332	(A)5→6	coding (744/1293 nt)	yjcF ←	pentapeptide repeat‑containing protein YjcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,282,332	1	.	A	100.0%	70.8 / NA	21	N248? (AAT→AAN)	yjcF	pentapeptide repeat‑containing protein YjcF
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (8/13);  total (8/13)

CGGTACATATAACCATTTCGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAA‑TTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATT  >  NC_000913/4282100‑4282580                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         cGGTACATATAACCATTTCGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCaaggaa                                                                                                                                                                                                                                         <  2:368530/251‑1 (MQ=255)     tcATATAACCATTTCGAGGAACGTATGTGTTAACCGCGTAAAAAAGAGTTTGCTAGCAATTAGGTTTATATTCGGGAGGGTTGCTTTAATCAGGTTGACACGAGGCAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGGCGGCTCTAAGGAAGTTAATATATGGTGCTATTACTTGGTAAAGAGTTGCCACAGTGAGGGATGCCATATAAAAAATTTGAATAATCAAGGAAGGt                                                                                                                                                                                                                                      <  1:439692/249‑1 (MQ=255)                      ggAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATTTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCATCCATTTTTGCCATATAAAAAATTTTAATAATCAAGGAAGGTATTTTTAATATTGGctct                                                                                                                                                                                                                    <  2:79554/251‑1 (MQ=255)                                     aCCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCAtt                                                                                                                                                                                                     <  2:227813/251‑1 (MQ=255)                                          gTAAAAAAGAGTTTGCTATCAATTAAGGTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGAGCAGCATGTTCAAAGTGAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATAGGGCGCTAATACGTTATAAAGATTTACCTCAGCCATTGAGGCCCGATAAAAAATTTGAAGAATCAAGCAAGGGATTTTTAATATGGGGTCTACTCATTGTGCCATTGTAGa                                                                                                                                                                                                <  2:404712/251‑1 (MQ=255)                                                      ttGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGc                                                                                                                                                                                     <  1:413414/250‑1 (MQ=255)                                                                taaaGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTAGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGACTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAAc                                                                                                                                                                           >  1:35152/3‑250 (MQ=255)                                                                               ggaaggTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAAATAAAACAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTGGAATAATCAAGGAAGGTATTTTTAATAGTGGCTCTATTCATTGTTGCATTTTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTcc                                                                                                                                                           <  2:91320/247‑1 (MQ=255)                                                                               ggAGGGTTGCTTTATTCAGGTTGACACGAGATAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGTTATTATTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATAGGGGCACTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGtt                                                                                                                                                             <  2:384007/249‑1 (MQ=255)                                                                                   ggTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCgtc                                                                                                                                                        >  1:351994/1‑250 (MQ=255)                                                                                                    tGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGc                                                                                                                                      >  2:352969/1‑251 (MQ=255)                                                                                                        acGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGaaa                                                                                                                                  <  1:141389/251‑1 (MQ=255)                                                                                                                       aTGTTCAACGTTAATTAAATCCAGTTTAGAGACGCTAAGGTCGGCTCTAAATAAATTAATATATGGCTCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTAAGTCATTTTTATCTGAGCGAAATTTGAGTCATCg                                                                                                                      >  2:128080/1‑248 (MQ=255)                                                                                                                            caacgTTAATTAAATCAAGTTTAGAGCAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTATATTCATTGTTGCATTGTAGAATTGAAAATGCATAGAGGAAAATGACATTAGAGTTCATTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATaa                                                                                                               >  2:333089/5‑249 (MQ=255)                                                                                                                                gTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTa                                                                                                          <  2:373361/251‑1 (MQ=255)                                                                                                                                       aaaTCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCaaaaa                                                                                                   <  2:351994/251‑1 (MQ=255)                                                                                                                                                      aaGCTAAGGTCGGCTTTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGTCAGGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTTCATTGTAGAATTGAACATTCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGTGAATACAATTATCAAAAAGTATTTTTTCCATTa                                                                                    <  2:6371/251‑1 (MQ=255)                                                                                                                                                                              ttAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTtat                                                            >  2:413885/1‑251 (MQ=255)                                                                                                                                                                                                  tttATAAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGaaa                                         >  2:29852/1‑250 (MQ=255)                                                                                                                                                                                                   tgataAAGATTTACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTAGTCATTGTTGCATTGGAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGTGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGTCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGaaa                                         <  2:266751/247‑1 (MQ=255)                                                                                                                                                                                                            tttACCTCAGCCATGTATGCCATATAAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACATTCCTGAAAAATAGCGtttt                              >  2:35747/1‑251 (MQ=255)                                                                                                                                                                                                                                atataaaaaa‑TTTGAATAATCAAGGAAGGTATTTTGAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAAt         <  2:15986/246‑1 (MQ=255)                                                                                                                                                                                                                                   taaaaaa‑TTTGAATAATCAAGGAAGGTATTTTTACTATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTCTCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCg      >  2:176003/3‑251 (MQ=255)                                                                                                                                                                                                                                       aaa‑TTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTTTCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGGCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAAtt  <  2:141028/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         CGGTACATATAACCATTTCGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAA‑TTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATT  >  NC_000913/4282100‑4282580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑