Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3630C.84.6% 78.3 / 10.2 26intergenic (‑490/+919)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base . (14/8);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 3.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01
*NC_0009132,173,3640C.84.6% 79.3 / 9.1 26intergenic (‑491/+918)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base . (14/8);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 3.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGC  >  NC_000913/2173121‑2173610
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                      
cAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACCGGGCGATGATCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGCTACCACC‑‑GCCGTATGt                                                                                                                                                                                                                                               >  2:356988/1‑251 (MQ=255)
            ccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGCTGGTGGCAAGATCGCCAAACGGCGGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAAc                                                                                                                                                                                                                                   >  1:389640/1‑251 (MQ=255)
                  gggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCgg                                                                                                                                                                                                                              <  1:167119/250‑1 (MQ=255)
                                              gATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTATGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc                                                                                                                                                                                                 >  1:31007/1‑251 (MQ=255)
                                              gATCCACAGGGAGATGCTCATAATGATGACACCCGCGATTAAGCTCCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCACACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc                                                                                                                                                                                                 >  2:59260/1‑251 (MQ=255)
                                                                aaaaaTGATGACCCCCGAGATTAAGGGGCGAAACAGATTCCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaaa                                                                                                                                                                                <  1:102212/247‑1 (MQ=255)
                                                                     tgatgaCACCCGATCTTAAGGTGCGGAACAGATTTCCACGTTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGCTACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGACCTTCGCCTGTAAACgcct                                                                                                                                                                           >  2:187532/1‑247 (MQ=255)
                                                                          aacccggaaaTTAAGATGCGTAACAGATTCCCAGGATGCCGGGCGCCCGCCATCGCCACAAAGAGGCCTTGGGTGGCAAAACCGCAAAAGGGCAGCCCCTGATTCCCCGGCACACAAACGGAAATTAAAATGGTGAGTGGGAAAAAAATCAGGTTTGCCGATGCCACC‑‑GCCGTATGTCCCAGGAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGATCTTCGCCTGTAAACGACTACGAGc                                                                                                                                                                     <  2:190733/242‑1 (MQ=255)
                                                                                        ggTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAACCGACTACGAGCCTGCTTAGCGATgg                                                                                                                                                       >  1:302446/1‑251 (MQ=255)
                                                                                           gCGGAACAGTTTCCCACGATGCACGGCGACCGCCACCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg                                                                                                                                                    <  1:235522/251‑1 (MQ=255)
                                                                                                          aCGATGCACGGCGACCGCCATCGACACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAAAGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGAGCGTTAAACCATCCatg                                                                                                                                      >  2:165496/1‑250 (MQ=255)
                                                                                                                   ggCGACCGCCATCGCCACGAAGAAGCCGATGGCGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTa                                                                                                                             >  1:38376/1‑250 (MQ=255)
                                                                                                                           ccATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGt                                                                                                                    <  1:431933/251‑1 (MQ=255)
                                                                                                                             aTCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTgg                                                                                                                  >  1:43013/1‑251 (MQ=255)
                                                                                                                              ccccaaagaaaaagacGATGGGGGCAAGATCGCCAAACGGAAACACCTGATTACCGGGCACAAAAACAGCAATAAAAGTGGGGAGGGGGAGAAAAACCAGGCTGGCAGTAACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGc                                                                                                                 <  2:302446/236‑1 (MQ=255)
                                                                                                                                             cGATGGTGGCAAGATCGACAAAGGGCAGCCCCTCATACCCCGCCACAGAAACAGAAATTAAAATGGTGAGTGGAAAAAAAAGAGGGCTGGCCGA‑ACC‑CCCCTCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTTGCATTAGCAGCATCACt                                                                                                  <  2:132812/251‑1 (MQ=255)
                                                                                                                                                     gCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGt                                                                                          >  1:12048/1‑251 (MQ=255)
                                                                                                                                                        aGATCGCCAAACGGCAGCACCTGATTACCCGGCAAACAACCAACAATTAAAATGGGGAGTGGGAAAAAAATCAGGCTTGCCGATAC‑‑CTCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAACCAGGAACTCCTTACCGCCGAACTGCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAATCCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGtttt                                                                                       <  1:427307/251‑1 (MQ=255)
                                                                                                                                                                     gcagcaCCAAATTACCCGGCCCAAAAACGGCAATAAAAAGGGTGAGTGTGGTAAAAGTCGGGCTGCCCGATGC‑‑CCCCGCCGTCTGCCCCAGCAGCAACGCCGGATCAAGGCTAATCAGGAACTCCGGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAATCCATCCATGATGGGTTTAATCACCCGTGGCATGAGCGGCATCTCTGCCGCCGTTGTTACCGCCAGCTGc                                                                          <  2:389640/251‑1 (MQ=255)
                                                                                                                                                                                      ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGCACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATc                                                         >  1:237849/1‑251 (MQ=255)
                                                                                                                                                                                          cacaAAAAGCAATTAAAATGGTGAGTGGAATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTaa                                                     <  1:418942/251‑1 (MQ=255)
                                                                                                                                                                                                      ttAAAATGGGGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGACGAACTTCGCCTGCAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATACATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACAACGTAACCCGCGAGGATg                                         >  1:298936/1‑251 (MQ=255)
                                                                                                                                                                                                           agggTGAGGGGGAAAAAAATCAGCCTTGCCAAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGAGGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGAt                                    <  1:356988/249‑1 (MQ=255)
                                                                                                                                                                                                                       aTAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc                                >  1:153685/1‑243 (MQ=255)
                                                                                                                                                                                                                               cAGGCTGGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTGGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCTGATAATCAGCCCCATCACAAAAc                <  1:323631/251‑1 (MQ=255)
                                                                                                                                                                                                                                    ttGCCGATACCACC‑‑GCCGACTGTCCCAGAAGCAACCCCAGATCAAGGCCAATCAGGAACTCCTGACCCCCGCACATCACCTGTCACCGACTCCGCCCCTGCTTCACGATGTGCGTTAAACCATCCATGATGGCTTTAAACACCCGTAGCACTCGCAGCCTAACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACCTCGCACCCCTCGAGGATGCCGATCATCAGCCCCCTCACAAAAcccccc           >  2:380468/1‑248 (MQ=255)
                                                                                                                                                                                                                                               cccccccCGTGTGTCCCAGAAGCAGCGCGGGATCAGGGCCAATCGGGAACCCCTGACCGCCGACCTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGTTGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGc  <  2:262057/250‑1 (MQ=255)
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                      
CAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGC  >  NC_000913/2173121‑2173610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑