Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,336,4110GA97.4% 133.8 / ‑3.6 39coding (84/1260 nt)murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/20);  minor base . (0/1);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
*NC_0009133,336,4120AT97.4% 137.6 / ‑6.1 39I28N (ATC→AAC) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (18/20);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGAT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGAC  >  NC_000913/3336187‑3336652
                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                                 
cgcgcTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGcc                                                                                                                                                                                                                         >  1:505351/1‑251 (MQ=255)
           cGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGa                                                                                                                                                                                                              <  1:130066/251‑1 (MQ=255)
                  aGCGCCTAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGcc                                                                                                                                                                                                       >  1:152376/1‑251 (MQ=255)
                        cAGATAGAAGCACGCATGGGTTTAACCAGATCGTAAGGTGCGCAGAATACATGAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCTCTGGAg                                                                                                                                                                                                 <  2:345367/251‑1 (MQ=255)
                         aGATAGAATCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATTCATTATCGTCGCTGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCACTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAATAGATT‑AGGCAGAGCGGCATTTTTAACGCCGGAAAGTGGGGCTTCGCCTTGGAGc                                                                                                                                                                                                <  2:381991/251‑1 (MQ=255)
                             aGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTc                                                                                                                                                                                             <  1:357502/250‑1 (MQ=255)
                                aGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGGCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTg                                                                                                                                                                                         <  2:507495/251‑1 (MQ=255)
                                     gCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGcccc                                                                                                                                                                                    >  1:49347/1‑251 (MQ=255)
                                          gTTTTAACCAGATCGTAAGGTGCTCAGAATCCATTAACGTCGCGGGCATCAATATGCACAGAACCAGTACTTTCTACTTTCGCACCCAGCTGGCTTAGCATCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGTAGCTTCGTTGGCCCCTGAac                                                                                                                                                                               <  2:94104/251‑1 (MQ=255)
                                          gTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAac                                                                                                                                                                               <  2:152376/251‑1 (MQ=255)
                                              tAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACg                                                                                                                                                                            <  1:235505/250‑1 (MQ=255)
                                              tAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGACATCAATATGAACCGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCGGCTTCATTGATGTCTCGACGTCTTTCAGATTAGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACg                                                                                                                                                                            >  1:185234/1‑250 (MQ=255)
                                                       cGTAAGGGGCGTAGAATACATTAACGGCTCGGTCATCAAGATGCACAGAACCATTACGTCCTACTTTCGCACCCAGCTGGCGTAGCAGCTTAAGTGATGTATCGACGTCTTGCAGTTTCGGGAGGATATGGAGCTCTACCGGTTCTACCGCCAGTAGGGCGGCAAAAAG‑ATGAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCGCTGGATCCTCGTTGGCCCCTGAACACGaaaggtatcc                                                                                                                                                                  <  2:190338/251‑8 (MQ=255)
                                                            ggTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGCCGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGCAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGCAATTTATCCAttt                                                                                                                                                              >  2:263934/1‑250 (MQ=255)
                                                                            tAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAACTTTATCCATTTAGTTTGGTCTCATTTa                                                                                                                                              >  1:402705/1‑250 (MQ=255)
                                                                             aaCGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAAc                                                                                                                                            >  1:370494/1‑251 (MQ=255)
                                                                              aCGTCGCGGGCATCAATATTCACAGAACAATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGATTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCCGTAGTGCGGCAACAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGACATTTATCAATTTAGTATGttaccctttatcc                                                                                                                                           >  2:49891/1‑240 (MQ=255)
                                                                                 gcgcgGGCATCCATATGCACAGACCCATTCCGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCCTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAAAACGAAATTTATCCATTTAGTTTGCTCTCAGTTAACAAtg                                                                                                                                        <  2:121064/250‑2 (MQ=255)
                                                                                       gcttCAATATGCACAGAACCATTACCTTCTATTTTCGCACCCAGCGGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCTCTGAACACGAAATTTATCCATTTAGTTTGTTCGCAGTTAACAATTCATATc                                                                                                                                  <  2:464314/248‑1 (MQ=255)
                                                                                         aTCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCg                                                                                                                                >  2:379740/1‑251 (MQ=255)
                                                                                           cAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGc                                                                                                                               <  1:529101/250‑1 (MQ=255)
                                                                                            aaTATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCt                                                                                                                              >  1:73999/1‑250 (MQ=255)
                                                                                                           aTTACGTTCTACTTTAGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGccc                                                                                                               >  1:420786/1‑250 (MQ=255)
                                                                                                                gTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCt                                                                                                         <  1:413427/251‑1 (MQ=255)
                                                                                                                  tCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCGACCGGCGAATCGCCCATAGCTc                                                                                                        <  2:505351/250‑1 (MQ=255)
                                                                                                                          cGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGAAGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCACAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGt                                                                                               >  1:267566/1‑251 (MQ=255)
                                                                                                                             ccccAGCTGGCGTATCAGCTTCATTGACGTATCCACGTCTTCCAGTTTCGGGACGGTCTGTATCTCTCCCGGGTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTTACTTCTCCCGTTAGCTTCGTTGGCCCCTGAACACTAAATTTATCCATTTAGTTTGTTCGCAGTTAACAATTCATATCCTCTACCGGCGAATCGCCCATAGCTCAAAAGCCGGTCa                                                                                            <  2:111225/250‑1 (MQ=255)
                                                                                                                                 aGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGttt                                                                                        <  1:304628/251‑1 (MQ=255)
                                                                                                                                                     tGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTcc                                                                     <  1:263934/250‑1 (MQ=255)
                                                                                                                                                           aTCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAggg                                                               <  1:224016/250‑1 (MQ=255)
                                                                                                                                                             cgactgcTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGt                                                             <  2:462670/244‑1 (MQ=255)
                                                                                                                                                             cGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGt                                                             <  2:49347/250‑1 (MQ=255)
                                                                                                                                                                      tCAGTTTCGGGACGTTCTGGCTCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGCAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTg                                                   >  2:277653/1‑251 (MQ=255)
                                                                                                                                                                                 aCGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGc                                        >  1:540120/1‑251 (MQ=255)
                                                                                                                                                                                              ctACCGGTTCTGCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGc                                <  2:17508/246‑1 (MQ=255)
                                                                                                                                                                                                    gTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGc                      >  1:248645/1‑250 (MQ=255)
                                                                                                                                                                                                     ttcttcCGCCAGTAGAGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATAGCGCGCCCACTCCGCAGGGGGATACGCTTTGATCGACACAGCATGAATGCGGTTATACGCa                     >  1:513735/1‑250 (MQ=255)
                                                                                                                                                                                                                      cGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTCGCGCAGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAACAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCgg    >  1:299920/1‑250 (MQ=255)
                                                                                                                                                                                                                       ggCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACAAGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCCGTTTGCGATCGCGCGACCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGAc  >  1:22103/1‑251 (MQ=255)
                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                                 
CGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGAT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGAC  >  NC_000913/3336187‑3336652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑