Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,088,0100CT96.0% 84.5 / NA 25intergenic (+150/‑274)metK/galPmethionine adenosyltransferase/galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (13/11);  minor base G (0/1);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
*NC_0009133,088,0110CT100.0% 91.6 / NA 25intergenic (+151/‑273)metK/galPmethionine adenosyltransferase/galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/12);  total (13/12)

AACCGCAGCATACGGTCACTTTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACT  >  NC_000913/3087767‑3088202
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                               
aaCCGCAGCATACGGTCACTTTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTac                                                                                                                                                                                           >  2:154093/1‑251 (MQ=255)
         agaCGGTCACTTTGGTCGTGACCATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCCCCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACcac                                                                                                                                                                                  <  2:387934/249‑1 (MQ=255)
            cGGTCACTTTGGTCGGGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCacaac                                                                                                                                                                               <  2:107590/251‑1 (MQ=255)
                cACTTTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGa                                                                                                                                                                            <  1:130610/250‑1 (MQ=255)
                  cTTTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGCTTATCATCACAACTGAAATTGATTACACCAACCACAACAGACa                                                                                                                                                                          >  2:65624/1‑250 (MQ=255)
                    ttGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACGGACaaa                                                                                                                                                                        <  1:154093/250‑1 (MQ=255)
                              aCATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAata                                                                                                                                                             <  1:245333/251‑1 (MQ=255)
                              aCATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAat                                                                                                                                                              >  2:120105/1‑250 (MQ=255)
                                        tGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCCTCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCAtatt                                                                                                                                                   >  1:168467/1‑251 (MQ=255)
                                                aaCCGCCAAAGCGCAGCTGCTGCGCGAGGCTGCCGGTCTGAAGTAATCTTTCTCCACCTGCTTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTAGTCg                                                                                                                                           <  2:305140/251‑1 (MQ=255)
                                                      cAAAGCGCATCTGCTGCGCTATGCTGCCGGGCTTAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTAGCATCACAACTTAAAGTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTc                                                                                                                                     <  2:449175/251‑1 (MQ=255)
                                                              aGCTGCTGCGCGATGCGGCCGGTCTGAAGTAATCTTTCTTCCCCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAgttgt                                                                                                                             <  1:462389/251‑1 (MQ=255)
                                                                       gcgatgtggcCGGTCTGAAGTAATCTTTCCTCACCGGCGTTCAAAGTCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCTGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACATCTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCt                                                                                                                    <  1:190464/243‑1 (MQ=255)
                                                                              tgcCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGt                                                                                                             >  1:469285/1‑251 (MQ=255)
                                                                                      tGAAGTAATCTTTCTTCACCTGCGTTCAACGGCCAACCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCCCAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTAGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCtt                                                                                                     >  2:541460/1‑251 (MQ=255)
                                                                                                tttcttCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGc                                                                                               >  1:461477/1‑247 (MQ=255)
                                                                                                 ttcttcACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAg                                                                                             <  1:116749/248‑1 (MQ=255)
                                                                                                      cACCTGAGATCAACGGCCAGCCTCAAGCAGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCACAACAACCGCCCGCCCAATAACATCATTCTTCCTGCTCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACACAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGc                                                                                     >  2:430818/1‑251 (MQ=255)
                                                                                                                                                     aGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAAt                                       <  1:89016/250‑1 (MQ=255)
                                                                                                                                                           tCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATACCATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAg                                                                                                                                 >  1:496027/1‑154 (MQ=255)
                                                                                                                                                                        ggCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGtt                    >  1:189525/1‑250 (MQ=255)
                                                                                                                                                                                  ccgcccgcACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCg          >  1:17623/1‑250 (MQ=255)
                                                                                                                                                                                         cacaATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACt  >  1:394319/1‑251 (MQ=255)
                                                                                                                                                                                                                                   atcatcACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTat                                                                          >  2:192316/1‑137 (MQ=255)
                                                                                                                                                                                                                                   atcatcACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAAGTGCAGATAACAGCGTTTAATCTat                                                                          <  1:192316/137‑1 (MQ=255)
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                               
AACCGCAGCATACGGTCACTTTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACT  >  NC_000913/3087767‑3088202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑