Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,608,416 G→A A656T (GCC→ACC)  zntA → Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,4160GA97.0% 207.7 / NA 69A656T (GCC→ACC) zntAZn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (33/32);  minor base C (0/2);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGGGCGTCAAAGGGGTGATCCTCACCGGCGATAATCCACGCGCAGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGAT  >  NC_000913/3608171‑3608660
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
cgcTGGGCGTCAAAGGGGTGATCCTCACCGGCGATAATCCACGCGCAGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGAc                                                                                                                                                                                                                                                 >  1:272504/1‑251 (MQ=255)
   tGGGCGACAAAGGGGTGATCCTCACCGGCGATAATCCACGCGCAGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGATGAATCAACATGCGCCGCGGGCGATGGTCGGTGACGGTATTTACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGAATGCAACGGGTAGCGGCACAGTCGTGGCGCTGGAAACCACCGACgc                                                                                                                                                                                                                                               >  2:307712/1‑250 (MQ=255)
    gggCGTCAAAGGGGTGATCCTCACCGGCGATAATCCACGCGCAGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACgca                                                                                                                                                                                                                                              <  2:378495/250‑1 (MQ=255)
          cAAAGGGGTGATCCTCACCGGCGATAATCCACGCGCAGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTa                                                                                                                                                                                                                                        <  1:185306/250‑1 (MQ=255)
             aGGGGTGATCCTCACCGGCGATAATCCACGCGCAGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAAccc                                                                                                                                                                                                                                    >  1:279023/1‑251 (MQ=255)
                  ggacccTCACCGGCGATAATCCACGCGCATGGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGTGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGGGCCAGCGATGAAAGCGGCCGCCATCGGGAGTGCAATGGGTAGCGGCACAGAGGTGGCGCTGGAAACCACCGACGCAGCATTATCCCATAac                                                                                                                                                                                                                               <  2:446653/247‑1 (MQ=255)
                    aTCCTCACCGGCGATAATCCACGCGCAGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAacca                                                                                                                                                                                                                             <  1:128766/251‑1 (MQ=255)
                     tcaggaCCGGCGAGAATCAAAGAGCAGGGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCACTGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAaccac                                                                                                                                                                                                                            >  1:179457/6‑251 (MQ=255)
                      ccTCACCGGCGATAATCCCCGCGGCGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAaccacc                                                                                                                                                                                                                           <  1:282515/251‑1 (MQ=255)
                          aCCGGCGATAATCCACGCGCAGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCGGGAAACCACCGACGCAGCATTAACCCATAACCACCTgcg                                                                                                                                                                                                                       <  1:325611/251‑1 (MQ=255)
                                    aTCCACGCGCAGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGACGATAAAGTCACAGCGGTGACAGAGCTGAATCAACATGCGCCGCTGGCGATGGGCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCACTGGGTAGCGGCTCAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTg                                                                                                                                                                                                             >  1:525163/1‑251 (MQ=255)
                                         cgcgCAGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAAt                                                                                                                                                                                                        <  1:44993/251‑1 (MQ=255)
                                                 agcggcAATTGCCGGGGAGATGGGGCTGGAGTATAAAGCGGGCCTGTTGCCGGAAGATAAAGCCAAAGCGGTGACCGAGCTGAATCAACATGCGCAGCTGGCGATGGTCGGTGACGGTATTTACGACCCGCCCGCGATGCAAGCTGCCGCCACCGGGCTTGCCATGGGTAGTGGCACAGACGTGGCGCTGGAAACAACCGCCGCCGCATTAACCCATCACCACATGCGCGGCCTGGAGCAAATGATTgcac                                                                                                                                                                                                >  2:203288/2‑248 (MQ=255)
                                                   cggcAATTGCCGGGGAGCTGGGGCTGGAGTTTACAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGCCCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTg                                                                                                                                                                                              >  1:305665/1‑251 (MQ=255)
                                                             cGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCa                                                                                                                                                                                    <  1:287085/251‑1 (MQ=255)
                                                              ggggAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGCGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGACAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTCACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCAc                                                                                                                                                                                   >  2:405038/1‑251 (MQ=255)
                                                                ggAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGATGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAAATGGCACGCGCCACTc                                                                                                                                                                                 >  2:3104/1‑251 (MQ=255)
                                                                 gAGCTGGGGCTGGAGTTTAACGCGGGCCTGTTGCCGGAAGATCAAGTCACAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGAAGGTATTAACGACGCGCCCGCGATGAAAGCTGCCGCCATAGGGATTGCCATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGGGCAAATGATTGACCTGGCACGCGCCAccac                                                                                                                                                                                >  2:248853/1‑248 (MQ=255)
                                                                   gatGGGGCTGGAGTTTAAGGGGGGCATGTTGCCGTAAGGAAAAGTCAAAGCGGTGACCAAGCTGAAGCAACATGCGCCGCTGGGTATGGTGGGTGACGGTATTAACGACGCGCCGGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACGGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCATTCACg                                                                                                                                                                              <  2:292813/249‑1 (MQ=255)
                                                                     tGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGAACGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAACCCACCGACGCAGCATTAACCCATAACCACCTGCGCGGACTGGTGCAAATGATTGAACTGGCACGCGCCCCTCACGcc                                                                                                                                                                            >  2:80697/1‑251 (MQ=255)
                                                                      ggggCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGGGGCGCTGGAAACCACCGAAGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGcc                                                                                                                                                                            <  1:405038/250‑1 (MQ=255)
                                                                      ggggCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGCCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCTCTCACGCCa                                                                                                                                                                           <  2:508957/251‑1 (MQ=255)
                                                                            ggattttAAAGCGGGCCTGTTGCCGGAAGATAAAGTAAAAGCGGTGACCGTGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGAGGGTATTAACGACGCGCCAGCGAGGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCCCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCTCGCCAATATcc                                                                                                                                                                     <  2:415706/247‑1 (MQ=255)
                                                                                 ttAAAGCGGGCCGGTGGCCGGAAGATAAAGTCAAAGCGGTGACCGATCTGAATCAACATGCGCCGCTGGCTATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGAAAACCTCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCa                                                                                                                                                                 <  2:341974/250‑1 (MQ=255)
                                                                                              gTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGCCGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACATACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTAt                                                                                                                                                     <  1:349834/249‑1 (MQ=255)
                                                                                                tGCCGGAAGATAAAGTCAAAACGGTGCCCGAGCTGAATCAACATGCGCCGATGGAGCTGTCCGGTGACGGTCTTCACGCCGCGCCAGCGATGAAAGCTGACGCCCTCGGGATTGCCATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCCTTAACCCATAACCACCTGCGCGGCCTGGTGCAACTGATTGACCTGGCACGCGCCCCTCACGCCAATATCCGCCAGACCATCACTAttatg                                                                                                                                                 >  2:319533/1‑248 (MQ=255)
                                                                                                   cGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTg                                                                                                                                              <  1:406046/251‑1 (MQ=255)
                                                                                                       aGATAAAGCCAAAGCGGTGACCGAGCTGAATCAACCTGCGCCGCTGGCGATGGTCTGGGGCGGTATTAACGGCGCGCCGGCGATGAAAGCCGCCGCCATCGGGATTGCAATGGGTAGCGGCCCAGACGTGGCGCGGGAAACCACCGACGCAGCATTACCCCATACCCCCCTGCGCGGCCGGTTGCAAATGATTGATCGGGCACGCGCCACTCACGCCAATATCCGCCAGAACAGCACTATTGCGCTGGGGc                                                                                                                                          <  2:272504/251‑1 (MQ=255)
                                                                                                         aTAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCAATCGGGACTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTACCCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTg                                                                                                                                        >  1:325919/1‑251 (MQ=255)
                                                                                                                 aaaGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGAt                                                                                                                                >  1:536487/1‑251 (MQ=255)
                                                                                                                   aGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATc                                                                                                                                                                      >  1:135330/1‑211 (MQ=255)
                                                                                                                   aGCGGAGACCGAGCTGAATCAACATGCGCCGCAGGCAACGGAAGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCAATCGGGATTGCAATGGGTAGCGGCACAGCCGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGCACATCAATATTGCGCTGGGGCTGAAAGGGATCt                                                                                                                              >  2:518552/1‑251 (MQ=255)
                                                                                                                     gggTGACCGAGCTGAACCAACTTGCGCCGCTGGCGATGGGCGGTGACGGTATTAACGACGCGCCAGCGAGGAAAGCGGCCGCCATCGGGATTGCAAGGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATACCCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTc                                                                                                                            <  2:6641/250‑1 (MQ=255)
                                                                                                                         gACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTc                                                                                                                         <  1:420957/250‑1 (MQ=255)
                                                                                                                           ccGAGCTGAATCACCATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGTCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACGATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTc                                                                                                                      <  1:346604/251‑1 (MQ=255)
                                                                                                                            cGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGGTCTTCCTCGTcc                                                                                                                     >  2:8045/1‑250 (MQ=255)
                                                                                                                                 tGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACg                                                                                                                >  1:129958/1‑251 (MQ=255)
                                                                                                                                  gAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACGGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTcaccac                                                                                                                 <  1:435146/249‑1 (MQ=255)
                                                                                                                                                 gcTGGCGATGGTCGGGGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCAGGAACCCATAACCACCGGCGCGGCCTGGTGCAAATGAGTGAACGGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCg                                                                                                <  1:500484/251‑1 (MQ=255)
                                                                                                                                                        agggTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGt                                                                                             <  1:433422/245‑1 (MQ=255)
                                                                                                                                                        aTGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAACGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGt                                                                                             >  2:433422/1‑247 (MQ=255)
                                                                                                                                                          ggTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGtggc                                                                                       >  1:337249/1‑251 (MQ=255)
                                                                                                                                                               gtgccgggattaccggcgcgCCAGCAAGGAAAGCGGACGCCATCGGGATTGCAAGGGTTAGCGGCACAGACGTGGCGCTGGAACCCACCGACGCAGCATTACCCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCCCGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGTATGACCGGGTTGTGGCTGGCa                                                                                  <  2:342161/235‑1 (MQ=255)
                                                                                                                                                                 gACGGTATGAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATGGCAAGGGGTAGCGGCACAGGCGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGa                                                                                                                                                                                                  <  1:302813/137‑1 (MQ=255)
                                                                                                                                                                             gacgCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGCCCGGGTTGTGGCTGGCAGTGCTGGCAGGTAc                                                                    >  2:283917/1‑251 (MQ=255)
                                                                                                                                                                             gacgCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATAc                                                                    <  1:341920/251‑1 (MQ=255)
                                                                                                                                                                             gacgCGCCAGAGAGGAAAGCTGCCACCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATAc                                                                    <  1:427832/251‑1 (MQ=255)
                                                                                                                                                                               cgcgCCAGCGATGAACGCTGCCGCCATAGGGATTGCACTGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACAACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACgg                                                                  >  2:485036/1‑251 (MQ=255)
                                                                                                                                                                                 cgcCAGCGAGGAAGGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCCCCTGCGCGGCCTGGTGCAAAGGATTGAGCTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGGTAGGGATGACCGGGTTGTTGCTGGCAGTGCTGGCAGATACgggg                                                                <  2:536487/251‑1 (MQ=255)
                                                                                                                                                                                     aGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGAGGGCGa                                                            <  1:150313/251‑1 (MQ=255)
                                                                                                                                                                                       cGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACg                                                          >  1:238910/1‑251 (MQ=255)
                                                                                                                                                                                        gATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACgg                                                         >  1:17638/1‑251 (MQ=255)
                                                                                                                                                                                          tGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCAAAGACGTGGCGCTGGAAACCACCGACGAAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTg                                                       >  1:346266/1‑251 (MQ=255)
                                                                                                                                                                                                    cgccATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGGTCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAg                                             <  1:8045/251‑1 (MQ=255)
                                                                                                                                                                                                    cgccATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGAc                                               >  1:486073/1‑249 (MQ=255)
                                                                                                                                                                                                          cGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACGGGCCCGCGCCACTCACGCCAAGATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTGCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAAt                                        <  1:248853/250‑1 (MQ=255)
                                                                                                                                                                                                           gggATTGCAAGGGGTAGCGGCACAGATGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGc                                      <  1:3104/251‑1 (MQ=255)
                                                                                                                                                                                                             gcttGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATACCCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCCCGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCCCCACGCTGTTAGGGATGACCGGGTTGTGGATGTCAGTGCTGGCCGATCCGGGGGCGCCggggtggtgatcggaggagcgtc                                    >  2:420622/3‑230 (MQ=255)
                                                                                                                                                                                                             gATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCTCCGCCGCAGCATTAACCCATACCCCCCTGCGCGGCCTGGTGAAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTg                                                    <  1:420622/235‑1 (MQ=255)
                                                                                                                                                                                                             gATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGGGCTGGTGACAGCGAATGCGt                                    >  1:124054/1‑251 (MQ=255)
                                                                                                                                                                                                                    aTGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGAtt                             <  1:283917/251‑1 (MQ=255)
                                                                                                                                                                                                                    aTGGGTAGCGGCACAGACGTGGAGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGAtt                             >  1:162477/1‑251 (MQ=255)
                                                                                                                                                                                                                     tGGGTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACCTCACTATTGAGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGGGCTGGCAGATACGGGGGCGACGGTGCTGGAGACAGCGAATGCGTTAAGAttg                            >  1:354591/1‑251 (MQ=255)
                                                                                                                                                                                                                        gTAGCGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCCCCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCTGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGAttgttg                         <  1:207517/251‑1 (MQ=255)
                                                                                                                                                                                                                            cGGCACAGACGTGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGATGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGAGACAGCGAATGCGTTAGGCTTGTTgcgcc                     >  2:128448/1‑250 (MQ=255)
                                                                                                                                                                                                                             ggCACAGACGTGGCGCTGGAAACCCCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCat                    <  1:368566/251‑2 (MQ=255)
                                                                                                                                                                                                                             ggCACAGACGTGGCGCGGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAg                    <  1:368561/251‑1 (MQ=255)
                                                                                                                                                                                                                                acaGACATGGCGCTGGAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCCAGTGATTGAACTAGCACGCGCCACTCACTCCCATATCCGCCAGAACATCAATATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGCAGGTGCTGGTGACAGCGAATGCGTTAAGATTGATGCACAggcg                 >  2:480561/1‑249 (MQ=255)
                                                                                                                                                                                                                                               gAAACCACCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGAt  >  1:73117/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
CGCTGGGCGTCAAAGGGGTGATCCTCACCGGCGATAATCCACGCGCAGCGGCGGCAATTGCCGGGGAGCTGGGGCTGGAGTTTAAAGCGGGCCTGTTGCCGGAAGATAAAGTCAAAGCGGTGACCGAGCTGAATCAACATGCGCCGCTGGCGATGGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGAT  >  NC_000913/3608171‑3608660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑