Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,554,539	T→G	Y485* (TAT→TAG)	malT →	DNA‑binding transcriptional activator MalT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,554,539	0	T	G	100.0%	208.7 / NA	63	Y485* (TAT→TAG)	malT	DNA‑binding transcriptional activator MalT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/33);  total (30/33)

CAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCAAACCGCGTGGGAAACGCAGGAAAAAGCATTCCAGCTGATCAACGAGCAGCATCTGGAACAGCTG  >  NC_000913/3554293‑3554782                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     cAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGcc                                                                                                                                                                                                                                                  <  1:73845/251‑1 (MQ=255) cAGCGTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGAGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACACTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGcc                                                                                                                                                                                                                                                  <  2:119515/251‑1 (MQ=255)  aGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCg                                                                                                                                                                                                                                                 >  1:479641/1‑251 (MQ=255)             aaaTCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCa                                                                                                                                                                                                                                       >  1:187255/1‑250 (MQ=255)                 ccGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGACGACACCATGCACGCAGAATTTAACGCTCTGCGCCACCAGGTGGCGATTAACGAAGGTAATCCGGATGAAGCGGAACGGCTGGGAAACCTGGCAATGGAAGAGCTGCCGCCGGGCTGGGTCTAGAGCCGCATTGTGGCAACCTc                                                                                                                                                                                                                                  >  2:98425/1‑251 (MQ=255)                         ggTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCaa                                                                                                                                                                                                                                      <  2:83019/239‑1 (MQ=255)                         ggTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCaa                                                                                                                                                                                                                                      >  1:83019/1‑239 (MQ=255)                            gtTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTTCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACGGGCACTGGAAGAGCTGCCGCCTGGCTGGTTCTAGAGCCGCAGTGTGGCAGCCTCGGTGCTGGGTg                                                                                                                                                                                                                       <  2:269375/251‑1 (MQ=255)                                        gTGGCTGATGCAAAGCCAACATCGCTACGGCGGAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCAAGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGTCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGctgcact                                                                                                                                                                                                           >  1:502514/1‑251 (MQ=255)                                          ggCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGGGCTGGGTGAAGTGctgcactgc                                                                                                                                                                                                         >  2:70516/1‑251 (MQ=255)                                          ggCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGAGGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCTCATTGTGGCAACCTCGGTGCTGGGTGAAGTGctgctctgc                                                                                                                                                                                                         <  2:501387/251‑1 (MQ=255)                                                    aaGCCAACATCGCTACGGCGAAGTTAACAACCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAAt                                                                                                                                                                                               >  2:335471/1‑251 (MQ=255)                                                        cAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGAc                                                                                                                                                                                           >  2:423723/1‑251 (MQ=255)                                                                  aCGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTg                                                                                                                                                                                 >  1:320622/1‑251 (MQ=255)                                                                  aCGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAAGCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCAc                                                                                                                                                                                   <  1:429426/249‑1 (MQ=255)                                                                       gAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAAGCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCt                                                                                                                                                                            <  2:166900/251‑1 (MQ=255)                                                                                 cccTTCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGGTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAGCGGCTGGCAAAACTGGCGCTGGAAGATCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGGGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCaa                                                                                                                                                                  <  2:530428/251‑1 (MQ=255)                                                                                 cccTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCaa                                                                                                                                                                  <  2:423679/251‑1 (MQ=255)                                                                                     gCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAAcc                                                                                                                                                               <  1:252924/250‑1 (MQ=255)                                                                                        aGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGaa                                                                                                                                                            <  1:44391/250‑1 (MQ=255)                                                                                             gTGCTGAACATGAAATCAAGGACATCAGAGAAGACCCCATGCACGCAGATTTGAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGACGAGCTGCCGCCGGGCTGGTTCTAGAGCAGCATTGGGGCAACCTCGGTGCTGTGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCCCTGGCGCTAATGCAGCAAACCGCACAGATg                                                                                                                                                      <  2:461482/251‑1 (MQ=255)                                                                                               gCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑Gc                                                                                                                                                   >  1:290490/1‑251 (MQ=255)                                                                                                   aaCATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTATGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGc                                                                                                                                               >  1:513911/1‑251 (MQ=255)                                                                                                         aaaTCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCAc                                                                                                                                         <  1:163039/251‑1 (MQ=255)                                                                                                         aaaTCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCAc                                                                                                                                         <  1:240988/251‑1 (MQ=255)                                                                                                               aGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTc                                                                                                                                   <  1:536243/251‑1 (MQ=255)                                                                                                                ggACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGAGCCCAGGAGGCGCTTAACGATGGTAATCAGGCTGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGATGCCGCCGGGCTGGTCCTAGAGCCGCCTTGTGGCACCCTCGGGGCTGGGTGAAGTGCTGCACTGCACAGGCGAATTGACCCGCTCACTGGCGCTCATGCAGCAAACCGCACAG‑TGTGCACGCCaccccgaggcct                                                                                                                                  >  2:483343/1‑240 (MQ=255)                                                                                                                 gACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTg                                                                                                                                 <  2:256554/251‑1 (MQ=255)                                                                                                                 gACATCAGAGAAGACACCATGCACGCAGAATTGAACGCTCTGCGCTGCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACGGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTg                                                                                                                                 <  2:349417/251‑1 (MQ=255)                                                                                                                 gACAGCAGAGAAGATACCAGGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATGAACGATGGGAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAGCCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTTACTGGCGCTATTGCAGCAAACCGAACAGATG‑GCACGCCAGCATGATGTCTg                                                                                                                                 <  2:256548/251‑1 (MQ=255)                                                                                                                  aCATCAGAGAAGACACCATGCACGCAGAATTTAACGCTATGCGCGCCCAGGTGACGATTAACGATGGAAATCCGGATGAAGCGGAACGGCTGGCAAAACAGACACTGGAAGAGCTGCCGCCGGGCTGGTCCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGAGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGATAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTgg                                                                                                                                >  1:219909/1‑251 (MQ=255)                                                                                                                     tCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCAc                                                                                                                             >  1:348269/1‑251 (MQ=255)                                                                                                                                acCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGAGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAAGTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTtgtg                                                                                                                  <  2:141787/251‑1 (MQ=255)                                                                                                                                   aTGCACGCAGAATTTAACGCTCGGCGCGCCCAGGGGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCGCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAg                                                                                                               <  1:483343/251‑1 (MQ=255)                                                                                                                                      cACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGCTTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCCACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCAAGCACGATGTCTGGCACTACGCTTTGTGGAGttt                                                                                                            >  2:361698/1‑251 (MQ=255)                                                                                                                                      cACGCAGAATTTAACGCTCTGCGCGCCCAGGGGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCGCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTTGAGttt                                                                                                            <  2:326528/251‑1 (MQ=255)                                                                                                                                         gCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAAt                                                                                                         <  2:479641/251‑1 (MQ=255)                                                                                                                                            gAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATcc                                                                                                       >  1:283679/1‑250 (MQ=255)                                                                                                                                             aaTTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATcc                                                                                                       >  1:525802/1‑249 (MQ=255)                                                                                                                                             aaTTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATcc                                                                                                       >  1:217199/1‑249 (MQ=255)                                                                                                                                                    cGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTg                                                                                              <  1:437620/251‑1 (MQ=255)                                                                                                                                                           cgcgcCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCAGTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCt                                                                                       <  2:247943/251‑1 (MQ=255)                                                                                                                                                            gcgcCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGATCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGTTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTGCGCTGTGTGGAGTTTAATCCAGCAAAGTGAAATTCTg                                                                                      <  1:519070/251‑1 (MQ=255)                                                                                                                                                                ccAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTg                                                                                  >  1:144651/1‑251 (MQ=255)                                                                                                                                                                    gTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGccc                                                                               >  1:493625/1‑250 (MQ=255)                                                                                                                                                                          aTTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCGCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCGCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGGGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGtt                                                                        <  1:298636/251‑1 (MQ=255)                                                                                                                                                                                gATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCa                                                                  <  2:144651/251‑1 (MQ=255)                                                                                                                                                                                 aTGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCaa                                                                 <  1:447878/251‑1 (MQ=255)                                                                                                                                                                                 aTGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCa                                                                  >  1:521460/1‑250 (MQ=255)                                                                                                                                                                                  tGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCaa                                                                 >  1:541203/1‑250 (MQ=255)                                                                                                                                                                                      aaTCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCAAACcg                                                             <  2:290490/250‑1 (MQ=255)                                                                                                                                                                                        tCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCAAACCGCGt                                                          >  1:65013/1‑251 (MQ=255)                                                                                                                                                                                             aTGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCAAACCGCGTGGGa                                                      >  1:119013/1‑250 (MQ=255)                                                                                                                                                                                             aTGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCGGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCACACCGAACAGATG‑GCACGCCAGCCCGATGTCTGGCACTACGCGTTGTGGAGTTTAATCCAGCAAAGTGAACTTCTGTTTGCCCAAGGGTTCCTGCAAACCGCGTgggc                                                      >  2:151714/1‑249 (MQ=255)                                                                                                                                                                                              ggAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCAAACCGCGTGGGaaa                                                    <  2:409616/250‑1 (MQ=255)                                                                                                                                                                                                    ggAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCGGGATCTAGAGCCTCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACCCTTAGTGGAGTTTAATACAGCAAAGTGAACTTCTGTTTGCCCAATGGTTCCTGCAACCCGCGTGGGAAACgaggg                                              >  2:135995/1‑247 (MQ=255)                                                                                                                                                                                                       aCGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCAAACCGCGTGGGAAACGCAGGaa                                            >  1:339974/1‑250 (MQ=255)                                                                                                                                                                                                             ggCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCAAACCGCGTGGGAAACGCAGGAAAAAGCAt                                     >  1:272113/1‑251 (MQ=255)                                                                                                                                                                                                                                 agCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCAAACCGCGTGGGAAACGCAGGAAAAAGCATTACAGCTGATCAACGagc                   >  1:158104/1‑249 (MQ=255)                                                                                                                                                                                                                                  gCTGCCGCCGGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCAAACCGCGTGGGAAACGCAGGAAAAAGCATTCCAGCTGATCAACGagcagc                <  1:335471/251‑1 (MQ=255)                                                                                                                                                                                                                                       cgccgGGCTGGTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCaa                                                                                                  >  1:450616/1‑164 (MQ=255)                                                                                                                                                                                                                                       cgccgGGCTGGTTCTAGAGCCGCATTGTGGCAACCGCGGTGCTGGGTGAAGTGCGGCACGGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATTTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCaa                                                                                                  <  2:450616/164‑1 (MQ=255)                                                                                                                                                                                                                                                ggTTCTAGAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTTTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCAAACCGCGTGGGATACGCAGGAAAAAGCAGTCCAGTTGATCAACGAGCAGCATCTGGAACAGCTg  <  2:498510/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATG‑GCACGCCAGCACGATGTCTGGCACTACGCTTTGTGGAGTTTAATCCAGCAAAGTGAAATTCTGTTTGCCCAAGGGTTCCTGCAAACCGCGTGGGAAACGCAGGAAAAAGCATTCCAGCTGATCAACGAGCAGCATCTGGAACAGCTG  >  NC_000913/3554293‑3554782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑