Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,436,697 G→A E60E (GAG→GAA)  trkA → NAD‑binding component of Trk potassium transporters

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,436,6970GA98.7% 259.3 / NA 78E60E (GAG→GAA) trkANAD‑binding component of Trk potassium transporters
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (34/43);  minor base C (1/0);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTG  >  NC_000913/3436453‑3436925
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    
aaGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAggg                                                                                                                                                                                                                                >  1:147718/1‑250 (MQ=255)
  gggggCGACGGCTTCTTTTACGCTAAGCTAATCAAAGAGGGCTGAGATAACGGGTCGCGACTGATGAAAATTATCCTTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCCACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGc                                                                                                                                                                                                                              >  1:134858/3‑251 (MQ=255)
  gagGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCAGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGGCGTGCAGGGGCATGGCTCTCCTCCACGCGTATTGCGGGAAGCAGGTGc                                                                                                                                                                                                                              >  1:134870/1‑251 (MQ=255)
   agGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAATTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGcc                                                                                                                                                                                                                             <  2:9171/251‑1 (MQ=255)
     ggCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCAACCACGCGTATTGCGGGAAGCAGTTGCcga                                                                                                                                                                                                                           <  2:438074/251‑1 (MQ=255)
      gCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGAGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGACGCAGGTGCcgac                                                                                                                                                                                                                          >  1:46170/1‑251 (MQ=255)
        gACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCcgacg                                                                                                                                                                                                                         >  1:531467/1‑250 (MQ=255)
               tcttTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGat                                                                                                                                                                                                                 >  2:127699/1‑251 (MQ=255)
                     aCGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCGGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTGACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCt                                                                                                                                                                                                            <  2:389233/250‑1 (MQ=255)
                       gctaagctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGCTATGCTGGt                                                                                                                                                                                                         >  1:164916/1‑251 (MQ=255)
                          aagcgaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGTTATGCTGGTTg                                                                                                                                                                                                       <  2:147884/250‑1 (MQ=255)
                           agctaaTCAAAAAGTGATGAGGTAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCTGGCCAGGTTGGCGGCACAATGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATTCCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGTGGCATGGCTCTTATCCACGCGTATTGCGGGAAGCAGGTGCCTACGACGCCGATATGCTGGTTGCt                                                                                                                                                                                                     <  1:499023/251‑1 (MQ=255)
                            gctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTg                                                                                                                                                                                                    <  1:177151/251‑1 (MQ=255)
                                    aaaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTCTCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGCACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCCGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGt                                                                                                                                                                                            >  1:316175/1‑251 (MQ=255)
                                      aaGTGATGAGATAACGGGTCGCGACTGCTGAAAATTATCATCCTGGGTGCAGGCCAGGTTGGCGGCACACTGGAGGAAAACCTGGTTGGCGAGAACAACGATCTTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCCCTAATACACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAggtc                                                                                                                                                                                          >  2:65022/1‑248 (MQ=255)
                                      aaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTc                                                                                                                                                                                          <  2:15577/251‑1 (MQ=255)
                                           atgaGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATg                                                                                                                                                                                     <  1:118031/251‑1 (MQ=255)
                                           atgaGATAACGGGCCGCGACTGATGAAAATTATCATTCTGAGTGCCGACCAGGTTGGCGGCACACTGGCGGAAAAACTGGTTGGCGAGAACAACGATATTACTGTAGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGAAGCCGATATGCTGGTTGCTGTAACCAGTTAAGATg                                                                                                                                                                                     >  2:540198/1‑251 (MQ=255)
                                                           cgACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgc                                                                                                                                                                     >  1:279305/1‑251 (MQ=255)
                                                           cgACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgc                                                                                                                                                                     >  1:370290/1‑251 (MQ=255)
                                                             aCTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGGCGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATTTGGTtgccg                                                                                                                                                                   >  2:521377/1‑250 (MQ=255)
                                                                gatgaAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcctg                                                                                                                                                                  <  1:208372/249‑1 (MQ=255)
                                                                            cattctcgccgCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAAAAACGCTATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTAAAGGATAAATTTGACATGCGGGTAGTGCCGGGGCATGGCTCTCATCAACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCCATTAAGATGCAACCAATATGGATGACTGCCAGGGAGCCTACt                                                                                                                                                    >  2:517955/11‑251 (MQ=255)
                                                                             aTTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGTAGCAGGTGCCGACGACGACGATATGCTGGGTGCTGTAACCAGTTCAGATGAAACCAATATGGGTGCCTGCCAGGTAGCCTACt                                                                                                                                                    >  2:295383/1‑250 (MQ=255)
                                                                               tCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGGCGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAATCCAATATGGTTGCCTGCCAGGTAGCCTACTCa                                                                                                                                                  <  1:517955/250‑1 (MQ=255)
                                                                                  gggTGCCGGCCAGGTTGGCGGCACTCTGGCGGAAAGCCTGGTTGGCGAGAACAACGATATTACTGTTGTAGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGGCCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGAGGCCGATATGCTGGGTGCTGTAACCAGTTCAGATGAAACCAATATGGTGGCCTGCCAGGTAGCCTACTCGCtt                                                                                                                                               <  2:316175/250‑1 (MQ=255)
                                                                                  gggTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTGTGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCGTGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGTTAGCCTACTCAcgtt                                                                                                                                              <  2:341216/251‑4 (MQ=255)
                                                                                             aGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCt                                                                                                                                   <  1:295383/251‑1 (MQ=255)
                                                                                             aGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGGTGCTGTAACCAGTTCAGATGAAAACAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCt                                                                                                                                   >  2:119635/1‑251 (MQ=255)
                                                                                              ggtgggcggcACTCTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACGTTTGTCGATACCAACGGTGAGAGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCAGCCACTCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTa                                                                                                                                  <  2:134870/247‑1 (MQ=255)
                                                                                                           cTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATGCCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGTAACCa                                                                                                                                                                               <  2:363991/193‑1 (MQ=255)
                                                                                                           cTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCa                                                                                                                                                                               >  1:363991/1‑193 (MQ=255)
                                                                                                           cTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCg                                                                                                                     >  1:15018/1‑251 (MQ=255)
                                                                                                             ggCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCg                                                                                                                     <  2:15018/249‑1 (MQ=255)
                                                                                                                       cTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCAcc                                                                                                         >  1:163834/1‑251 (MQ=255)
                                                                                                                         ggTTGGAGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCAACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCAcc                                                                                                         >  2:358498/1‑249 (MQ=255)
                                                                                                                                 agaACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGAGGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTg                                                                                               <  1:530139/251‑1 (MQ=255)
                                                                                                                                   aacaacGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTgcc                                                                                             >  2:181939/1‑250 (MQ=255)
                                                                                                                                     caacGATATTACTGTTGTCGATCCCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGcgcg                                                                                           <  2:531467/251‑1 (MQ=255)
                                                                                                                                       acGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGAt                                                                                         <  1:131036/251‑1 (MQ=255)
                                                                                                                                              tACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGGTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGAt                                                                                   <  2:198084/250‑1 (MQ=255)
                                                                                                                                                    tgtCGATCCCAGCGGTGGGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAATCCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTAGTATCCGCTCAACAGACTACGTGCGCGATGCCGATAAGCTa                                                                             <  2:411921/250‑1 (MQ=255)
                                                                                                                                                          tACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATttcatt                                                                      >  1:368293/1‑251 (MQ=255)
                                                                                                                                                           aCCAACGGTGAGCGTCTGCGGACCGTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATttcatt                                                                      <  1:450834/250‑1 (MQ=255)
                                                                                                                                                            ccAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATttcattca                                                                    <  1:375829/251‑1 (MQ=255)
                                                                                                                                                              aaCGGTGAGCGTCGGCGGACCGTACAGGATAAATTTGACCTGCGGGTCGTGCAAGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATAGGCTGGTTGCTGTAACCAGTTCATATGAAACCAATATGGTTGCCGGCCAGGTAGCCTTCTCTCTTTTCAACACCCCTAATCTCATCGCTCGTATCCGCTCATCAGACTACGTGCGCGATGCCGATAAGCTATGTCATTCAGa                                                                  <  2:520772/251‑1 (MQ=255)
                                                                                                                                                                 ggTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGc                                                               <  2:394291/251‑1 (MQ=255)
                                                                                                                                                                  gTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCt                                                              <  1:119635/251‑1 (MQ=255)
                                                                                                                                                                     ggCGTCTTCGTACCTTACAGGATAAATTGGACCTGCGGGTCGTGCAGGGGCAGGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGGTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCGCGGATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCAGTCATATGCtgtg                                                           <  2:510773/250‑1 (MQ=255)
                                                                                                                                                                            gCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGa                                                       <  1:521377/248‑1 (MQ=255)
                                                                                                                                                                            gCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGAtt                                                     <  2:365108/250‑1 (MQ=255)
                                                                                                                                                                             cGGACCTTACAGGATAAATTTGCCCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTg                                                    >  1:160198/1‑250 (MQ=255)
                                                                                                                                                                             cGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGt                                                                                                                                                                                            <  1:30533/114‑1 (MQ=255)
                                                                                                                                                                             cGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGt                                                                                                                                                                                            >  2:30533/1‑114 (MQ=255)
                                                                                                                                                                               gACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGatc                                                 >  1:209532/1‑251 (MQ=255)
                                                                                                                                                                                aCCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGatc                                                 >  1:51446/1‑250 (MQ=255)
                                                                                                                                                                                    tACAGGATAAATTTGACCTGCGGGTCGTGGAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCGATTTCATTCAGATGCTGTGCCGATTGATCATCt                                             <  1:446970/250‑1 (MQ=255)
                                                                                                                                                                                    tACAGGAGAAATTTGAACTGCGGGGCGTGCAGGGGCATTGCTCTTACCCACGCGTATTGCGTGAAGCAGGTGCCGACGACGCCGATATGCTGGGTGCTGTAACCAGTTCAGATGAACCCAAGATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGatcata                                              <  2:7836/249‑2 (MQ=255)
                                                                                                                                                                                       aGGATAAATTTGACCTGCGGGTCGTGCAGGTGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATTAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATc                                         <  2:370290/251‑1 (MQ=255)
                                                                                                                                                                                       aGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATc                                         >  1:269260/1‑251 (MQ=255)
                                                                                                                                                                                           tAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTAATCCCCGCGTATTGCGGGAACCAGGTGCCGACGACGCCGATATGCTGGTTGATGAAACCAGTTCAGATGACACCAATATGGTTGCCTCCCCGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCAc                                     >  1:245195/1‑251 (MQ=255)
                                                                                                                                                                                            aaaTTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCAcc                                    <  1:127699/251‑1 (MQ=255)
                                                                                                                                                                                             aatgtGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCGCGTATCCGc                                                                                                              <  2:510668/172‑1 (MQ=255)
                                                                                                                                                                                             aaTTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGc                                                                                                              >  1:510668/1‑176 (MQ=255)
                                                                                                                                                                                                 tgaacttctggTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCTCCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCagag                                <  2:90912/241‑1 (MQ=255)
                                                                                                                                                                                                 tGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCagag                                <  1:270830/250‑1 (MQ=255)
                                                                                                                                                                                                  gACCGGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGc                               <  2:356277/250‑1 (MQ=255)
                                                                                                                                                                                                    ccTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGt                            >  1:122137/1‑251 (MQ=255)
                                                                                                                                                                                                              gTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCg                   >  1:389889/1‑250 (MQ=255)
                                                                                                                                                                                                                   ggggCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAatcgcatc                                                                                                                          <  1:250766/142‑1 (MQ=255)
                                                                                                                                                                                                                      gcatggacctcATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCCATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGCCTACGTGCGCGATGCCGATCAGCTCTTTAATTTAGCTGCTGTGCCGATTGATCATCTGATCGCTCCAGAGCAGTTTGTTATCGCAAATAttt          >  2:190997/9‑251 (MQ=255)
                                                                                                                                                                                                                        aTGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAAGCGCAGCGCTCGTATCCGCTCACCAGACTACGTGCGCGAGGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTTATCATCTGATCGCACCAGAGCATTTGGTTATCGATAATATTTAc        <  2:272930/251‑1 (MQ=255)
                                                                                                                                                                                                                          ggCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTAcc       <  2:163958/250‑1 (MQ=255)
                                                                                                                                                                                                                           gCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGAAGCCGATATGCTGGTTGCTGTAACCAGTTAAGATGAAACCAATATGGTTGCCTGCCAGGAAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCg      >  1:526326/1‑250 (MQ=255)
                                                                                                                                                                                                                            ctctCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGCTGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGAc    >  2:192175/1‑251 (MQ=255)
                                                                                                                                                                                                                              ctcATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAacac                                                                                                                                       <  2:173208/118‑1 (MQ=255)
                                                                                                                                                                                                                              ctcATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAacac                                                                                                                                       >  1:173208/1‑118 (MQ=255)
                                                                                                                                                                                                                                cATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCGGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTg  <  1:463297/249‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    
AAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTG  >  NC_000913/3436453‑3436925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑