Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,614,394	G→A	G154D (GGC→GAC)	focB →	formate channel FocB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,614,394	0	G	A	93.0%	126.5 / NA	45	G154D (GGC→GAC)	focB	formate channel FocB
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base A (16/24);  minor base C (2/1);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 5.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGGCAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTC  >  NC_000913/2614160‑2614623                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      ccggtgtgtttttGCTGGCGGGTTGCGGCACCAGCCTGTTCACCTCGTTGGTAATGAGGGTGATGGCAAAAAGTCGGGGTGTTATTAGGTGGCGAACTTTGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTGGTCCGGGCTGGTGCTGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTCCACTGCGCCGATGCCAAAATGCATCATACa                                                                                                                                                                                                                       <  2:408484/242‑1 (MQ=255)              gCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGGCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACACACTTCTTGTGGCCTGCGGGAATCTGGCAGGGATTGCCTGTTTCAGGTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGGTTTACACTGCGCCGAGGGCAAAATGCATCATACATTTTCTGAAtttg                                                                                                                                                                                                          >  1:525823/1‑248 (MQ=255)                       ttGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTc                                                                                                                                                                                                  <  1:18108/250‑1 (MQ=255)                       ttGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTc                                                                                                                                                                                                  <  2:447605/250‑1 (MQ=255)                         gCGGCACCAGCATGTTCACCTCGTCGGTAATGAAGGCGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGTACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGCAAACGCGATGTGGGGAGTCGCGGTTTTTCACTGCGCCGAGGACAAAATGCATAATACATTTACTGAATCTTTCAGCCTCgcc                                                                                                                                                                                               >  2:433774/1‑249 (MQ=255)                           ggCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACGTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCAt                                                                                                                                                                                             <  1:433774/251‑1 (MQ=255)                                 aGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTAtgtg                                                                                                                                                                                       <  2:7795/251‑1 (MQ=255)                                   ccTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGc                                                                                                                                                                                      >  1:383334/1‑250 (MQ=255)                                      gTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATc                                                                                                                                                                                  <  1:281643/251‑1 (MQ=255)                                           ccTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGGATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACAATGCGCCGAGGCCAAAATGCATCATACATTTACTGAAGCTGTCAGACTCGGCATTATGTGCAATCTGATg                                                                                                                                                                             >  2:382552/1‑251 (MQ=255)                                                 cgGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTg                                                                                                                                                                        <  1:457785/250‑1 (MQ=255)                                                 cgGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGc                                                                                                                                                                       >  1:90489/1‑251 (MQ=255)                                                                           cGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCg                                                                                                                                              <  1:531032/250‑1 (MQ=255)                                                                            ggggCTTTAGTAGTTGGCGATCTTGGCTGATTAACGCATTGCTTGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAAGTCATCATACATTTGCTGAATCTGTCAGCCGCGGCTTTATGTGCAATCTGATGGTTTGCCTGGCGCGGTGGATGAGTTATTGCggg                                                                                                                                            <  2:38365/251‑1 (MQ=255)                                                                                            gCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGAc                                                                                                                             >  1:405600/1‑250 (MQ=255)                                                                                                    gtcTGATTAGCGCATTTTGGGTGGCCTGTGGTAATCGGGCATGTAGTGCCTGTTGCAGTTTGTGAATCTGGGTTGCCGGGCTGGTGAGGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTGCACTGCGTCGAGTACAAAATGCATCATACATTTACTGACTCTTTCAGCCTCGGCTTTATGTGCAGTCTGATGGTTTTCCTGGCGCTGTGGAGGAGGTTTTGCGGGCATTCGTTATGCGACTAAAtcga                                                                                                                     <  2:196480/248‑2 (MQ=255)                                                                                                        gATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTCCTGAATCTGTCAGCCTCGGCTTTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATTGTCGcc                                                                                                                 >  2:302250/1‑250 (MQ=255)                                                                                                        gATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGcc                                                                                                                 >  1:348437/1‑250 (MQ=255)                                                                                                                    tCTGGTGGCCTGCGGTAATCTGGCAGGTATGGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCATCCTCGGCATTATGTGCAATCTTGTGGTTTGCCTGGCGCTGTGGATGAGTTATTTCTGGCCTTCGTTATGGGACAAAATCGTCGTCGTGATTTTGccc                                                                                                     <  2:348961/250‑1 (MQ=255)                                                                                                                      ggtgggCCTGCGGTAATCGTGCAGGTATTGCCTGTTTCAGTGTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATc                                                                                                  <  2:277531/247‑1 (MQ=255)                                                                                                                           gCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCt                                                                                             <  1:302250/251‑1 (MQ=255)                                                                                                                                   tAATCTGGCAGGCATTGACTCTTTCAGTTTGTTCCTCTAGATTTCCGGACTGGTGATGCGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGCCGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCACTCTGATGGTTTGCCTGGCGCTGCGGATGAGCTATTGCGGGCGTTCGTTCTGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCg                                                                                     >  2:507648/1‑251 (MQ=255)                                                                                                                                              gTATTGCCTGTTTCAGTTTGGTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACAGTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCt                                                                                                                                                                                 <  1:69196/148‑1 (MQ=255)                                                                                                                                              gTATTGCCTGTTTACGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAACCGCGCTGTGGGGAGTCGCGGTTTTCCACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCt                                                                                                                                                                                 >  2:69196/1‑148 (MQ=255)                                                                                                                                                aTTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTACGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTgg                                                                        >  2:111948/1‑250 (MQ=255)                                                                                                                                                 ttGCCTGTTTCAGTGTGTTAATCTGGTTGTCCGGGCTGGTGAGGAGTTAAAACGCGATGTGGGGAGTCGCGGTTTTACACGGCGCCGAGGGCAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTAGGTGCAATCTGATGGGTTGCCGGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTATGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGa                                                                        <  2:224235/250‑1 (MQ=255)                                                                                                                                                      tGTTTCAGTTTGTTAATCTGGCTTTCCGGGCTGGTGATAAGCGAAAACGCGATGTGGGGAGACGCGGTTTTACACTGCCCCGAGGCCAACATGCCTCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCCATCTGATGGTTTGCCTGGCGCTGTGGACGAGTTATTGCGGTCGTTCGTTATGCGACACAATCGTAGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTg                                                                  >  2:486595/1‑251 (MQ=255)                                                                                                                                                       gTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACt                                                                   <  1:277060/249‑1 (MQ=255)                                                                                                                                                        tttCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGccg                                                                                                                                                                                                   <  2:62023/120‑2 (MQ=255)                                                                                                                                                           cAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATc                                                              <  1:359355/250‑1 (MQ=255)                                                                                                                                                                         ggttttcacggatGGTGATGCGAGAAAACGGGATGCGGGAAGTCGCGATTTTACCATGCGACGTGGACAACATGCATCATACCTTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGACGCTGAGGATGAGTTATTGCGGGCGTTCGTTCTGCGACAAAATAGTCGCAATGATTTTGCCAATCACACTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCACTTTGTTtgtg                                               >  2:317749/13‑251 (MQ=255)                                                                                                                                                                                   gCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACGGTATCGCTAAGTTGTTTGTGATttcgttcg                                     <  2:477699/251‑1 (MQ=255)                                                                                                                                                                                         gatgaGTGAAAACGCGATGTGGGGCGTCGCGGTTTTCCACTGCGCCGAGGACAACATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGCGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCACTTTGTTTGTGATTCCGTTCgccattg                               >  2:407609/1‑251 (MQ=255)                                                                                                                                                                                         gatgaGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCgccattg                               <  1:407609/251‑1 (MQ=255)                                                                                                                                                                                         gatgaGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCgccatt                                <  1:102866/250‑1 (MQ=255)                                                                                                                                                                                                gAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATttgtttgt                                                >  2:148724/1‑227 (MQ=255)                                                                                                                                                                                                gAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATtagtttgt                                                <  1:148724/227‑1 (MQ=255)                                                                                                                                                                                                   aaCGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGCATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCCCCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCCTTCGccc                     >  2:358228/1‑250 (MQ=255)                                                                                                                                                                                                       cgATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGACGAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCCTTGCCATTCGCCATTTc                 >  2:504141/1‑251 (MQ=255)                                                                                                                                                                                                           gtgGGGAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTACGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGcc              <  1:111948/250‑1 (MQ=255)                                                                                                                                                                                                              gggAGTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTc          >  1:91324/1‑251 (MQ=255)                                                                                                                                                                                                                  gTCGCGGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAAACGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTccccc      <  1:358228/251‑1 (MQ=255)                                                                                                                                                                                                                      cgGTTTTACACTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTc  <  1:361276/251‑1 (MQ=255)                                                                                                                                                                                                                               acTGCGCCGATGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTACGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCgccat                                 <  2:408909/211‑1 (MQ=255)                                                                                                                                                                                                                               acTGCGCCGAGGACAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTACGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCgccat                                 >  1:408909/1‑211 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      TCGGCTTTATTTTGCTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGTCGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAATCTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAAAACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGGCAAAATGCATCATACATTTACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATGAGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTC  >  NC_000913/2614160‑2614623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑