Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,484,8270CT96.4% 92.5 / NA 29L152F (CTT→TTT) evgSsensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (15/12);  minor base G (0/1);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGG‑CACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGT  >  NC_000913/2484590‑2485065
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                
aCCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAAt                                                                                                                                                                                                                                    >  1:243510/1‑251 (MQ=255)
   gATTCGTAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCg                                                                                                                                                                                                                                 >  1:142478/1‑251 (MQ=255)
   gATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATggg                                                                                                                                                                                                                                 <  1:345561/251‑3 (MQ=255)
              aCGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGGTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCt                                                                                                                                                                                                                      <  2:381774/251‑1 (MQ=255)
                           aTTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGt                                                                                                                                                                                                         <  2:24553/251‑1 (MQ=255)
                                tGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATAGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACATCACCCACACCAGCAAat                                                                                                                                                                                                     >  2:140569/1‑250 (MQ=255)
                                tGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAACAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAat                                                                                                                                                                                                     >  2:387004/1‑250 (MQ=255)
                                          ttAAATCTTTTAACAAGAGCGATAAATATCAAATAAACACTCCGGAAATACACATATCAGCAAAAAGCAATGAAAGCGCTTGCAGAAGGTGAAGTCGAGAAAGAGTTATCACATTTAGTTACTTCGCCGCCACTTAATAATGACATTGCTGCAACCAAACCAATGCTAATTCCATTTACGGCGCTGGTAACCACCTTT‑CACGACTAAATGCGACCGCTTACATCACCAAAACAAGTAAATATTGCTCgga                                                                                                                                                                                           >  2:174162/1‑249 (MQ=255)
                                                 ttttAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAAt                                                                                                                                                                                   >  2:260816/1‑251 (MQ=255)
                                                               ttAAATATCAAATTAAAACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAACTTACCCCCCAgccg                                                                                                                                                                      >  1:362308/1‑247 (MQ=255)
                                                                         aaTTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATtcat                                                                                                                                                           >  1:396930/1‑251 (MQ=255)
                                                                            tAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATtcatca                                                                                                                                                         >  1:207477/1‑250 (MQ=255)
                                                                                            cGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCt                                                                                                                                                                                               <  1:10906/196‑1 (MQ=255)
                                                                                            cGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCt                                                                                                                                                                                               >  2:10906/1‑196 (MQ=255)
                                                                                                      caAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtct                                                                                                                               <  1:454550/250‑1 (MQ=255)
                                                                                                                   aCGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAttt                                                                                                                  >  1:336737/1‑250 (MQ=255)
                                                                                                                           gCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTGCCGGCGCTGGTAACCACCTTT‑CACGACGCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGAGGTGGTAATTCATCAATCAGTTCCAAAAGCAACAATTATCTCGTTTACAAATTTATATCAGGc                                                                                                         <  2:329956/251‑1 (MQ=255)
                                                                                                                                         cgaattaGTGGTATCACATTTAGTTACTTCACCGCCTCTTAATAATGACATTGCTTCAACCAAACCATTGATAATTACCTTTCCGGCTCTTGTAACC‑CCCTTGCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCGCTGTGACAAATTTATATCAGGCATTAGCagtcggc                                                                                            <  2:362308/245‑7 (MQ=255)
                                                                                                                                                       cacaTTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGG‑CACAAt                                                                            >  1:69950/1‑251 (MQ=255)
                                                                                                                                                                          cccTCTTAATAATGACAGTGCTTCAAACAAACCATGGATAATTTCCTTTCCGGCGCTTGTAACCACCTTT‑CTCTACTCAATGCTACCTCTGACCTCACCAAAACCAGTAAATATTTCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCGGTGACAAATTTATAGTAGGCATGAGCATCCGTCTCAG‑TGGGTCTCAATGATTatgttagcggtagta                                                         <  2:303311/250‑15 (MQ=255)
                                                                                                                                                                                 aataatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGG‑CACAATGATTACTTTATTGGTAGTAAcatca                                                   <  1:113893/250‑1 (MQ=255)
                                                                                                                                                                                             gctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGG‑CACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTa                                       <  1:421623/250‑1 (MQ=255)
                                                                                                                                                                                                              gATAAGTACCTTTCCGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCTCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGG‑CACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTAAGATTTCCCGCGATTtca                     <  2:243510/251‑1 (MQ=255)
                                                                                                                                                                                                                           ccGGCGCCGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGG‑CACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTAGGATTTCCCGCTATTTCACTCACTCCTTaa         <  2:69950/250‑1 (MQ=255)
                                                                                                                                                                                                                            cGGCGCTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGG‑CACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAAt       >  1:68687/1‑251 (MQ=255)
                                                                                                                                                                                                                                gcTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGG‑CACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGt     >  1:205364/1‑249 (MQ=255)
                                                                                                                                                                                                                                 cTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCa                                                                                                            >  1:475856/1‑146 (MQ=255)
                                                                                                                                                                                                                                 cTGGTAACCACCTTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCa                                                                                                            <  2:475856/146‑1 (MQ=255)
                                                                                                                                                                                                                                   ggTAACCACCGTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGATGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCTGCTGGG‑CACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGt  <  2:142478/249‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                
ACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTT‑CACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGG‑CACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGT  >  NC_000913/2484590‑2485065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑