Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,405,969	C→T	V225I (GTT→ATT)	lrhA ←	DNA‑binding transcriptional dual regulator LrhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,405,969	0	C	T	95.8%	152.1 / NA	48	V225I (GTT→ATT)	lrhA	DNA‑binding transcriptional dual regulator LrhA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (24/22);  minor base A (2/0);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGC  >  NC_000913/2405725‑2406213                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      aacaaTGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCgg                                                                                                                                                                                                                                                 >  1:315713/1‑251 (MQ=255)       gAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGACCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCCACCGGCCTTGCCGTCAC‑GCCCAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCgg                                                                                                                                                                                                                                           >  1:452264/1‑250 (MQ=255)                                    gCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGaa                                                                                                                                                                                                              <  1:395347/251‑1 (MQ=255)                                            gCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATGCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCt                                                                                                                                                                                                      <  2:411911/251‑1 (MQ=255)                                                   aTTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACAAGCAGGTCCGGGCTCATAAACTCAACCAGCCTTTCCGTCCC‑GCCAAGACCGGCTTTCACTGCCCCCCGACAGGCCGGGAACGTCGCGGCGACACAAGACAGGCGCCAAGGAatatttcaattatta                                                                                                                                                                                                >  2:542181/1‑239 (MQ=255)                                                                          gATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCACATATTCGGTATCAGGTCGCGGCGGTAAGACAAATACCCCACTCAGCAAACGCAGGTCCGGGCTCACCCTCTCCACCCGCCTTGCCGTCAC‑GCCAAGGCCGGCTTTCACTACCCCACGAAAGGCCGGGAGCGCCGAGGCGAACTAAGCCCGTCGCCCTGGGCTATCTGCTTTTTTACGCGTAGCCACCACCACATcgcgg                                                                                                                                                                        >  1:525519/1‑250 (MQ=255)                                                                              gataaCCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCTCCATATCGCGAAACg                                                                                                                                                                    <  2:101164/247‑1 (MQ=255)                                                                              gATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCCGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTCCCGCACGAATGGCCGGCAGCGGCGCGGCGACAAAAACCAGTCGCCATGGAAAATATGCTTTATTAAGGGGCGCCCACACCCTATCGCGGAACg                                                                                                                                                                    >  2:445989/1‑251 (MQ=255)                                                                                        gCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGGCCGGGCTCATCATCTCAACCGGCCTTGCCGTCCC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGGCGAGGCGACATAAGCCAGGCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGatc                                                                                                                                                          >  1:61223/1‑251 (MQ=255)                                                                                                   attaCTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGcc                                                                                                                                                                                                                           <  1:371446/175‑1 (MQ=255)                                                                                                                   tAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCAATCTACTCCACTCCGCACACGCAGGGCCGGGCTCATCATCTCAACCGGCCTTGCCGT‑ACAGCCCAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGGCGAGGCGACATAAACCAGTCGCCAAGGAATATCTGCTTTATTAAGCGTCGCCAACACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAAA‑GGGATCGGCTc                                                                                                                              >  1:238867/1‑251 (MQ=255)                                                                                                                           cAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCCCTCAGCCCACGCAGGGCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGCAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCttttt                                                                                                                      >  1:233637/1‑251 (MQ=255)                                                                                                                           cAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCCTCATCTCAACCGGCCTTTCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCCCGAATTGCCGGAAGCGTCGAGGCGACATACGCCCGTCTCCCTTGAatat                                                                                                                                                                                                           >  2:407910/1‑167 (MQ=255)                                                                                                                           cAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAatat                                                                                                                                                                                                           <  1:407910/167‑1 (MQ=255)                                                                                                                           cAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATAATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAACACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCttttt                                                                                                                      >  1:38122/1‑251 (MQ=255)                                                                                                                                atTCGGTATCAGGTAGCGGCGGTAAGCCATCCACAACACTCAGCACACGCCGGTCAGGAATCATCATCTCAACCGGCCTTGCCATCAC‑GCCCAAAACGGATTTCACTACCCCAAGAATGGCCGGGAGCGTCCAGGCGACATAAGCCAGTCGCCATGGGATATCCGACTTATTAAGCGTCGCCAGCACCATAACGCGAAACGGGCTAGGAACATCCAGCAATACCAGAAGGGA‑CGGCTCGCCCTTTTGCac                                                                                                                  >  1:75260/1‑249 (MQ=255)                                                                                                                                       aTCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCCGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCCAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGCGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGGCGCCAGCACCCTATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGGTCGGCTCGCCTTTTTTCAATATGtttt                                                                                                          >  1:254215/1‑248 (MQ=255)                                                                                                                                            gTAGCGGCGGTAAGCCATCTACTCCACCCAGCCCACGCAGGTCCGCGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCGTTTTGCAATATGTATTCCGCt                                                                                                     <  2:61223/251‑1 (MQ=255)                                                                                                                                                 ggcggTAAGCCATCTACTCCACTCAGCACACGAAGGCCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTgcgcg                                                                                                >  1:259237/1‑250 (MQ=255)                                                                                                                                                          ccATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCGTTATTAATCGTCGCCAGCACCATAGCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGAGCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAgtg                                                                                       <  1:240608/251‑1 (MQ=255)                                                                                                                                                           cATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATTTCTTCCGCTGCGCAGTACCAgtgt                                                                                      >  2:507016/1‑251 (MQ=255)                                                                                                                                                           cATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAgtgt                                                                                      <  2:111047/251‑1 (MQ=255)                                                                                                                                                                   ccACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCg                                                                               <  1:319603/250‑1 (MQ=255)                                                                                                                                                                     aCTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAgg                                                                            <  1:445989/251‑1 (MQ=255)                                                                                                                                                                      cccAGCACACGCAGGTCCGGGCTCAGCATCTCATCCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACAGAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCGCCATAGCTCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTGGCAATATGTATTCCGCTTCGCAGTACCAGTGTGTTGGCGAGGt                                                                           <  2:525519/249‑1 (MQ=255)                                                                                                                                                                       ccAGCACACGCAGTTCCGGGCTCAACATCGCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCGTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGGTTTGGCGAGGTa                                                                          <  2:315713/250‑1 (MQ=255)                                                                                                                                                                         aGCACACGCAGGGCCGGGCTCATCATCTCAACCGGCCTTGCCTTCAC‑GCCAAGACCGGCTTTCACGGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACg                                                                        <  2:233637/251‑1 (MQ=255)                                                                                                                                                                            acacGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAAAATGTTTTCCGCTTCGCAGGACCAGTGTGTTGGCGAGGTACGc                                                                       >  2:221282/1‑249 (MQ=255)                                                                                                                                                                             cacGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCTCTGCGCAGTACCAGTGTGTTGGCGTGGTTCGCAgg                                                                    >  2:398219/1‑251 (MQ=255)                                                                                                                                                                               cGCAGGCCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGtt                                                                  >  1:148322/1‑251 (MQ=255)                                                                                                                                                                                    gTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATg                                                             >  2:51625/1‑251 (MQ=255)                                                                                                                                                                                      ccGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCgg                                                                                                                                 >  2:379534/1‑181 (MQ=255)                                                                                                                                                                                      ccGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCgg                                                                                                                                 <  1:379534/181‑1 (MQ=255)                                                                                                                                                                                       cGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGcc                                                                                                                                                                                                                                                   >  2:247508/1‑67 (MQ=255)                                                                                                                                                                                       cGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGcc                                                                                                                                                                                                                                                   <  1:247508/67‑1 (MQ=255)                                                                                                                                                                                          gCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAAGATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTaa                                                       <  1:247806/251‑1 (MQ=255)                                                                                                                                                                                             catcatCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGACGCGACGCGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGCAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGACTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTTGCGAGGGACGCAGGGTCACTGCCTTAAACg                                                    >  2:43590/1‑251 (MQ=255)                                                                                                                                                                                             catcatCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACg                                                    <  2:452264/251‑1 (MQ=255)                                                                                                                                                                                                    ccAACCGGCCTTGCCGTCTC‑GCCAAGACCGTCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GTGATCGTCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTTTTGGCGAGGTACGCATGTTCAATGCCTTAAACGTTGAAgg                                             <  2:48492/250‑1 (MQ=255)                                                                                                                                                                                                          ggCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGACGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGT‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATg                                       >  1:211240/1‑251 (MQ=255)                                                                                                                                                                                                          ggCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCAACATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCACTATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATg                                       >  1:525573/1‑251 (MQ=255)                                                                                                                                                                                                           gCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGACGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTAGCCATGGAATAGCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGACTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTAAATGCCTTAAACGCTGAAGGAAGATgg                                      >  1:460131/1‑251 (MQ=255)                                                                                                                                                                                                              ttGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGggtg                                   >  1:262773/1‑251 (MQ=255)                                                                                                                                                                                                                    gcAC‑GCCAAGACCGGCTTTCACTGCCGCACGAATGGACGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTAcc                             <  1:274828/250‑1 (MQ=255)                                                                                                                                                                                                                                  ggCTTTCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGGATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACtt                >  1:234285/1‑251 (MQ=255)                                                                                                                                                                                                                                       tCACTGCCGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTg            <  1:132642/250‑1 (MQ=255)                                                                                                                                                                                                                                              cGCACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCa    <  1:542181/251‑1 (MQ=255)                                                                                                                                                                                                                                                cACGAATGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGc  <  1:43590/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      AACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCAC‑GCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGA‑GGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGC  >  NC_000913/2405725‑2406213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑