Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,395,372 G→A S175S (AGC→AGT)  nuoJ ← NADH:quinone oxidoreductase subunit J

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,395,3720GA91.9% 166.2 / ‑5.2 63S175S (AGC→AGT) nuoJNADH:quinone oxidoreductase subunit J
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (38/19);  minor base C (1/4);  total (39/24)
Fisher's exact test for biased strand distribution p-value = 5.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATC  >  NC_000913/2395127‑2395618
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       
ctcTTCTGCCGCCGCGAGGCTGATGGTGAGAATGTACAGCACCTGACCGTCGGTCTGGCCTCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAAGTAGATTGCC‑GCGAGGATCATTCCATGTGGTAAGTGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTg                                                                                                                                                                                                                                                      <  2:212742/247‑1 (MQ=255)
     cTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGAGCTCCTCCGTTTTTCTTTTCGCACTGTCGACtt                                                                                                                                                                                                                                               >  2:117799/1‑251 (MQ=255)
       gccgccGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTGAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCTTGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCt                                                                                                                                                                                                                                                <  1:313486/248‑1 (MQ=255)
            cgcgAGGAAGATGGCGAGAATGTACATCACCTGAACGAAGGTCTGGCGCCAGTAGCTTCCGGCGACCACGAAGGACAGCGCGGGGGCGTTAATCATGATTTCCAGACCAATCAACATAAAAAACAGATTGCGACGGATAACAAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCAATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGCCTTTACGAtt                                                                                                                                                                                                                                        >  2:121578/1‑251 (MQ=255)
              cgAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGc                                                                                                                                                                                                                                      >  1:512541/1‑251 (MQ=255)
                 ggCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGGCTTTACGATTGCTCa                                                                                                                                                                                                                                   <  1:475075/251‑1 (MQ=255)
                        ggCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTc                                                                                                                                                                                                                            >  1:367522/1‑251 (MQ=255)
                                 gTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGACAAGAACGAATAATATTGCC‑GCGAGGATCATTCCATGTTGTAAGGGGATCATGCTTGCTCCTCCGTTTTTCTTTTAGCACTGTCTTCTTTACGATTGCTAAGCAATTCACCCGCACg                                                                                                                                                                                                                   >  1:155930/1‑251 (MQ=255)
                                              ccGTCGGTCAGGCACCAGTAGCTTACGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAAAATAAAAAGCAGATTGCGACGGATAAACAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCACACTGTCGTCTTTACGCTTGCTCAGCACTTCCACCGAACGATTTTCAcgaccg                                                                                                                                                                                                      >  2:315045/1‑251 (MQ=255)
                                                  cGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCAc                                                                                                                                                                                                                    >  2:378474/1‑233 (MQ=255)
                                                  cGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCAc                                                                                                                                                                                                                    <  1:378474/233‑1 (MQ=255)
                                                   ggTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTg                                                                                                                                                                                                 >  1:131645/1‑251 (MQ=255)
                                                         gCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGc                                                                                                                                                                                           <  1:159525/251‑1 (MQ=255)
                                                             cAGTAGCTTCCGGCGACAACGAAAGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATACGATTGCC‑GCGAGGAACAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCcac                                                                                                                                                                                        >  2:351300/1‑250 (MQ=255)
                                                                 aGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAAcc                                                                                                                                                                                    <  1:106725/250‑1 (MQ=255)
                                                                               aCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAAGAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGagcagc                                                                                                                                                                    <  2:301523/251‑1 (MQ=255)
                                                                                    ggCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCAt                                                                                                                                                               <  1:135548/251‑1 (MQ=255)
                                                                                        aGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGCGCAGCAGCATAGa                                                                                                                                                            >  2:386185/1‑250 (MQ=255)
                                                                                              gAGGCGTTAAGCATGATTGCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATGGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACGGTCGTCTTTACGATTGCTCAGCACGTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGt                                                                                                                                                     <  2:514499/251‑1 (MQ=255)
                                                                                               aGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGtt                                                                                                                                                    <  1:114677/251‑1 (MQ=255)
                                                                                               aGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGtt                                                                                                                                                    >  1:117574/1‑251 (MQ=255)
                                                                                                ggCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTc                                                                                                                                                   >  2:11261/1‑251 (MQ=255)
                                                                                                  cGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTcc                                                                                                                                                  >  1:301378/1‑250 (MQ=255)
                                                                                                             aTTTCCCGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACg                                                                                                                                      >  1:359137/1‑251 (MQ=255)
                                                                                                             aTTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACg                                                                                                                                      >  1:486284/1‑251 (MQ=255)
                                                                                                                tCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCCCGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGAACGTa                                                                                                                                    >  2:403613/1‑250 (MQ=255)
                                                                                                                          aaaaCATAAACACAAGATTGCGACGAAAAACCAGACGGTTTAAGCAAAGAACGAATAGGATTGCC‑GCGAGGATCAGTCCTTGTTGTAGGGGGATCTTGCGTGCTCCTCCTTTTTTTTTTTCGCACTGTCTTTTTTAGGATTGCTCAGCACTCCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCACCTGAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACa                                                                                                                         <  1:420293/249‑1 (MQ=255)
                                                                                                                           caacaaAAACAGCAGATGGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCTTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCCCTTCCCCCGCACGCTCTTCACGACCGAGGGGGAAGGCCACAACCAGCCCGG‑CGATCAGCAGCATGGAATCCATTTCCACCGCCAGTACGTAAGGCCCGAACAg                                                                                                                        <  1:50955/251‑1 (MQ=255)
                                                                                                                             aCATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCg                                                                                                                      >  1:123312/1‑251 (MQ=255)
                                                                                                                                        agATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACt                                                                                                           >  2:368509/1‑251 (MQ=255)
                                                                                                                                          aTTGCGACGGATAACCGGCCCGGTTAAGCCAAGAAGAAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCTTGCGTGCCCCTCCTTTTTTCTTTTCGCACTGTCGTCTTTACGATGGCTCAGCACTTCACCCGCCCCCTCTTCACGCCCGACGGGGAAGGCCACAACCAG‑CCTGGCGAGCAGCAGCAAAGAGGCCTGTTCCTCCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGc                                                                                                         <  1:513236/251‑1 (MQ=255)
                                                                                                                                            tGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGCGGATCAGTCCATGTTGTAAGGGGATCATGAGTGCTCCTACGTTTTTCTTTTCGCAATGTCGTCTTTACGATTGCTCAGCCCTTCACCCGCACGCTCTTCACGACCGACGTGGACGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCtt                                                                                                       >  1:516252/1‑251 (MQ=255)
                                                                                                                                              cGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGCGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCttt                                                                                                      >  1:62260/1‑250 (MQ=255)
                                                                                                                                               gccGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGACGCCAGTTCCACCGCCAGAACGTAAGGCCCGAACAGCGTAATACCCACTGCtttcg                                                                                                    >  2:251599/3‑249 (MQ=255)
                                                                                                                                                        ccagccgtgTTAACCCAAGAACAAATAAGATGTCC‑GTAAGGTTCATTCCATGTTTTAGGGGGATCAGTCGTCCTCCTCCTTTTTTCTTTCCGCCCTGTCGTCTTTACGATTGCTCAGCACTCCCCCCGCCCGCTCTCCCCGACCGGCGGGAAAGCCCACACCCAGCCCTC‑CGGCCAGCAGCAAAGAAGCCAGTCCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTg                                                                                           <  1:391187/243‑1 (MQ=255)
                                                                                                                                                        ccagccagttaaaGCCAAAAACAAATAAG‑TTGCCCGCGAGAATAACTCCAGGTTGAGAGGGGATCTGGCGTGCTCCTCGTTTTTTCTTCTCGCCTTGTGGTCTTTACAATTGCACAGCCCCCCCCCCGCGCGCTCTTCACGACCGCGGGGGAAGGCCACACCCAGCCCTG‑CGGGCAGCGGAATAGAACCCATTCCCTCCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTg                                                                                           <  2:367522/240‑1 (MQ=255)
                                                                                                                                                         cataCCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGTATCAGTCCATGTTGTAAGGGGATCATGCGTCCTCCTCCGTTTTTCTTTTCGCACTGTCGCCTTTACGATTGCTCAGCACTCCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACACCAAGCCCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTgg                                                                                          <  1:524905/248‑1 (MQ=255)
                                                                                                                                                         cAGACCGGTCAAGCCAAGCACGAATAAGATTGCC‑GCGAGGATCAGTCCCTGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCCCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGAACGACGTGGCAGGCCACAACCAGACATG‑CGAGCAGCAGCATAGACGCCAGTTCCACCGCCAATACGTAAAGCCCGCACAGCGTAATACCCACTGCTTTatccctgcttgg                                                                                          >  2:248047/1‑240 (MQ=255)
                                                                                                                                                              gggtaaagcaaagaaaaaagaaGATGGCC‑GCGAGAATCAGTCATTGTTGTATGGGCATCTTGCGTCCCCCGTCTTTTTTCTTTCCCCCCTGTCTTCTTTACGATTGCTCAGCCCTCCCCCCCCCCCCTCTCCAGAACCGAGGTGAAAGCCCACACCCAGCCCGG‑CGAGAAGCAGAATAAACCCCATTACCTCCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTAc                                                                                     <  2:204704/231‑1 (MQ=255)
                                                                                                                                                               ggTTAAGCCAGGAACGAATAAGTTGGCC‑GCGAGGATCAGTCCATTTTTTAAGGGGATCTGGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCCCTTCCCCCGCACGCTCTCCACGCCCGACGTGGAAGGCCACAACCAGCCCTG‑CGAGCAGCAGCATAGAAGCCAGTCCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTAcc                                                                                    <  1:394831/251‑1 (MQ=255)
                                                                                                                                                                gTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCg                                                                                   >  1:256928/1‑251 (MQ=255)
                                                                                                                                                                  tAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCTTGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTCCCCCCGCACGCTCTTCACGACCGACGTGGAAGGCCACACCCAGCCCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCt                                                                                                        <  1:9428/228‑1 (MQ=255)
                                                                                                                                                                  tAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCt                                                                                                        >  2:9428/1‑228 (MQ=255)
                                                                                                                                                                   aaGCCAGGAACGAATACTATTGCG‑GCTAGTACCAGCCCATGTTTTAAGGGTTTCTTGCGTGCTCCCCCTTTTTTCTTTTCGCTCTGTCTTTTTTACGATTCCTCAGCACTCCCCCCCCGCGCTCTTCCCGCCCGGGGTGGAAGGCCACAGCCAGACCTT‑TGCCCAGCCGCACAGAAGCCTTTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTc                                                                                 <  1:316581/250‑1 (MQ=255)
                                                                                                                                                                    aGCCAAGAACGCATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCg                                                                                >  2:71963/1‑250 (MQ=255)
                                                                                                                                                                                    gATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCAACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAac                                                               >  1:16619/1‑251 (MQ=255)
                                                                                                                                                                                              gAGGATCAGCCCTTTTTGTAAGGGGTTCAGGCGGGTCCCTCCGTTTTTTTTTCCGCCCGTTCTTTGTTACGATTCCTCACCACTCCCCCCGCCCGCTCTCCACGCCCGCGGTGGAGGGCCACAACCAGCCCGG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGAt                                                      <  2:512541/251‑1 (MQ=255)
                                                                                                                                                                                                      gTCCATGTTGTAAGGGGATCATGCGTGCTCCTCAGCTTTTCTTTTCGCACTGTCGTCTTTACGATAACTCAGCACTTCACCCGCACGCTATTCACGACCCACGTGGCAGGACACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGCTACACTGCTCGTTAACACCGCGGATGGCGTaa                                               >  1:229622/1‑250 (MQ=255)
                                                                                                                                                                                                        ccATGTTGTAAGGGGATCATGCGCGCTCCTCCGTTTTCCTTTTCGCACTGTCGTCTTTACGATTGATCACCACTTCACCCGCACGCTCTTACCGACCGACGTAGACGGCCACAACCAGACATG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGAAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCCATGATTGGCGGACCGTCGATACCCTGCTCGTTAacacaggggagggagcaacc                                             >  2:502629/1‑234 (MQ=255)
                                                                                                                                                                                                        caaTGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTCCTCAGCCCTTCCCCCGCCCGCTCTTCACGCCCGACGTGGAAGGCCACAACCAGCCCGG‑CGAGCAGCAGAATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAAc                                             <  1:516155/248‑1 (MQ=255)
                                                                                                                                                                                                          aTGTTGTAAGGGGATCATGAGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGCAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAAt                                          >  2:219594/1‑251 (MQ=255)
                                                                                                                                                                                                            gttgtAAGGGGATCATGCGTGCTCCTCCGTTTTTATTTCCGAACTGAAGTCTTTAAGATTGCTCAGCACTTCACCCGCACGCTCTACCCGACCAACGTGGAAGGCAACAACCAGACCTG‑CGAGCAGAAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCACGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGCTCGTTAACACCGAGGATGGCGTAAACAATc                                         >  1:495624/1‑250 (MQ=255)
                                                                                                                                                                                                                  aGGGGAGCTTGCGTCCTCCTCCTTTTTTCTGTTCGCACTGCCGTCTTTACGATTGCTCAGCACTCCCCCCGCACGCTCTTCACGACCGACGTGAAAGCCCCCAACCACACCTG‑CGAGCAGCAGAATAAAAGCAAGTCCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCAccacca                                  <  1:44849/251‑1 (MQ=255)
                                                                                                                                                                                                                   ggggATCATGCGTGCTCCTCCGTTTTTATTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCaccacc                                   >  2:532563/1‑249 (MQ=255)
                                                                                                                                                                                                                     ggATCATGCGTGCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTACCACCGAGGATGGCGTAAACACTCACCACCAGc                                >  2:259871/1‑250 (MQ=255)
                                                                                                                                                                                                                         catgcgccctcctcCGTATCTATTTTCGCACTGTAGTATTTACGATTGCTAAGCACTTAACCCCCAAGCTCTTCACGCACGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCCGCATAGAAGCCAGTTACACCGCCAGTACGTAAGGCCCGACAAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCATGCTCGATAACACCGAGGATGGCGGAAACAATCACCACCAGCatgat                           >  2:348120/9‑251 (MQ=255)
                                                                                                                                                                                                                          aTGCGTGCTCATCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCatgatg                          >  1:408140/1‑251 (MQ=255)
                                                                                                                                                                                                                                gCTCCTCCGTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGAc                    >  2:202954/1‑251 (MQ=255)
                                                                                                                                                                                                                                        gTTTTTCTTTTCGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAACATTGc            >  2:312172/1‑251 (MQ=255)
                                                                                                                                                                                                                                         tttttCTTTTCGAACAGTCGACTTTACGATTGCTCAGCACTTCAACCGCACGCTCTTCACGACCGACGTGGAAGGCCACAAACCGACCTG‑CGAGCAGCAGCCTAGAAGCCAGTTCCACCGCCAGTACGGAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATAcc                                                                           >  2:328073/1‑187 (MQ=255)
                                                                                                                                                                                                                                           tttcttttcGCACTGTGCTCTTTACTATTGCCCACCACTCCCCCCGCCCCCTCTTCCCGCCCGACGGGGAAGCCCACACCCACCCCTG‑GGAGCAGCAGCATAGAAGCCATTCCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCg          <  1:117799/250‑1 (MQ=255)
                                                                                                                                                                                                                                                tttcGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCACCATGATGGCCGACAAAATTGCCGGCCCa     >  1:387409/1‑250 (MQ=255)
                                                                                                                                                                                                                                                  tcGCACTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGACCAGCGTAATACCCACTGCTTTAGCCCTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGCCCactc  >  1:287907/1‑248 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       
CGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCC‑GCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTG‑CGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAACAATCACCACCAGCATGATGGCCGACAAAATTGCCGGACCAATC  >  NC_000913/2395127‑2395618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑