Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,241,519	A→T	N50K (AAT→AAA)	galS ←	DNA‑binding transcriptional dual regulator GalS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,241,519	0	A	T	100.0%	228.0 / NA	67	N50K (AAT→AAA)	galS	DNA‑binding transcriptional dual regulator GalS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (33/34);  total (33/34)

CGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCGGCTATTTTGTCAGCACTGAATACAGGACTTCGTGAATCGAGTCACAG  >  NC_000913/2241273‑2241761                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   cgcCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTgg                                                                                                                                                                                                                                                >  1:69367/1‑251 (MQ=255) cgcCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACAAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTgg                                                                                                                                                                                                                                                >  1:310695/1‑251 (MQ=255)    caggtCATCGGCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTTGTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATTCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGGCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCAAGCGCCTGGGGTTTGGCGt                                                                                                                                                                                                                                             <  2:134736/247‑1 (MQ=255)     aGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGtt                                                                                                                                                                                                                                            >  1:288585/1‑251 (MQ=255)              tcactcacTGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGAt                                                                                                                                                                                                                                    >  2:149499/1‑250 (MQ=255)                 cTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATc                                                                                                                                                                                                                                >  1:406458/1‑251 (MQ=255)                        cTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGctc                                                                                                                                                                                                                          >  2:198074/1‑250 (MQ=255)                                tGAACAATCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGc                                                                                                                                                                                                                 >  1:201949/1‑251 (MQ=255)                                  aaaaaTCGACGCCTTACAACGCTGGCGAATTTAAC‑CCTCAATGGCCTTGCGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCtt                                                                                                                                                                                                               <  2:408587/248‑1 (MQ=255)                                      aTCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGCCCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCCGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCAt                                                                                                                                                                                                           <  1:523981/251‑1 (MQ=255)                                      aTCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGAGGCTGCTGAGCGACCAGATCCACCTCTTTTACCAGCGCGCCGAAAAGCGCATCAGTAACGTCCATCACCACCACGCCAATGGGGTCGCTAACCTGAGTTGCCAGCGCCTGGGTTTTGGCTTTTTGCCGATAATCCAGCTCACTCACTGCTTTCAt                                                                                                                                                                                                           <  1:51258/251‑1 (MQ=255)                                       tCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCAtt                                                                                                                                                                                                          >  2:375718/1‑251 (MQ=255)                                          aCGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCGGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCGCCACGCCAATTGTGGCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACt                                                                                                                                                                                                       <  2:505070/251‑1 (MQ=255)                                           cGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTg                                                                                                                                                                                                      >  2:209268/1‑251 (MQ=255)                                              aTTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCtt                                                                                                                                                                                                   >  1:344542/1‑251 (MQ=255)                                                    aCGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCAcgcg                                                                                                                                                                                             >  1:494366/1‑251 (MQ=255)                                                                  aaCACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGAc                                                                                                                                                                                >  1:115533/1‑251 (MQ=255)                                                                   acacCTCAATGGCGTGACGCTTTTTTTCCGATTCATGTTAGATATTGCCAATTAGACCGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACAAAGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGAcc                                                                                                                                                                               <  2:2279/251‑1 (MQ=255)                                                                      cctcaaatgcctgtcGCTCTTTTTCCGCTTCAGTAGAGCTATTGCCGATTAGCACGTATGTCTGATGCTTCTGAGTGAACAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACTCCCGTCGCCACCACGCCATTGGTTTCGCTAACCTGATTTGCCAGCGGCTGGGCTTGGGCGTTTGGCCGTTGATCCAGCGCACTCACTGGTTTCATTACTGCTCCGCGCGTGGCGGCACTGACCa                                                                                                                                                                              <  2:459969/235‑1 (MQ=255)                                                                       cTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGCTGCCAGCGCCTGGGCTTTGGCGTTTGGTCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCaacg                                                                                                                                                                           >  2:499886/1‑248 (MQ=255)                                                                           agggCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGAGCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCt                                                                                                                                                                       <  1:112263/249‑1 (MQ=255)                                                                           aTGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCt                                                                                                                                                                       <  1:499886/251‑1 (MQ=255)                                                                                gTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTAt                                                                                                                                                                  >  1:490782/1‑251 (MQ=255)                                                                                  gACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTg                                                                                                                                                                >  2:9008/1‑251 (MQ=255)                                                                                    cGCTCTTTTTCCGCTTCAGGATAGCTATTGCCGATTAGCACGTATTTCGGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCGCCACGCCAATGGTGTCGCTAACCTGAGTTGCTAGCGCCTGGGCTTTGGCGTTGGGCCGATAATCCAGCGCACTCACGGCTTTCATTACTGCTTCACGCGTGGCGGTACTGACCAGCGTGCTGTTATTGAg                                                                                                                                                              <  2:91704/251‑1 (MQ=255)                                                                                             tCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCggg                                                                                                                                                      >  1:520383/1‑250 (MQ=255)                                                                                                  ttCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTGCCAGCGCGCCGAAAAACGCATCAGAAGCTTCCATCACCACCACGCCAATGGTGTCGCTATCCTGAGTTGCCATCGCCTGGTCTTTGGCg                                                                                                                                                                                                                                              <  2:520760/157‑1 (MQ=255)                                                                                                  ttCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTCCTGCGCGACCAGCTCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGCAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCg                                                                                                                                                                                                                                              >  1:520760/1‑157 (MQ=255)                                                                                                        aTAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCca                                                                                                                                          <  1:347315/251‑1 (MQ=255)                                                                                                          aGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCCCGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAAccagctcgga                                                                                                                                         >  2:301859/1‑242 (MQ=255)                                                                                                          aGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAAcc                                                                                                                                                 <  1:301859/242‑1 (MQ=255)                                                                                                            cTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAAACGTTGCcac                                                                                                                                         >  2:2322/1‑248 (MQ=255)                                                                                                              aTTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTTGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAgaga                                                                                                                                    <  1:337804/251‑1 (MQ=255)                                                                                                              aTTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAAGCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACagag                                                                                                                                     <  2:408109/250‑1 (MQ=255)                                                                                                                  ccGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAgccgcc                                                                                                                                >  2:200643/1‑251 (MQ=255)                                                                                                                     attggCACGTAGTTCTGATGCGGCTGATCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAAGCCAGCTCACTCACGGCGTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGc                                                                                                                             <  2:115533/247‑1 (MQ=255)                                                                                                                          cACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGTGCTTTGGCTTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGAc                                                                                                                        <  1:55124/251‑1 (MQ=255)                                                                                                                                  tCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTAc                                                                                                                 <  1:9008/250‑1 (MQ=255)                                                                                                                                     gATGCTGCGGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCTCCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATGGAGCGCCCGGGAAGCCGGTGCCACAGAGACGCCAGCCGGACGCGCTACATc                                                                                                              <  2:490782/250‑1 (MQ=255)                                                                                                                                      aTGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAACCCGTTGCCCCAGAGACGCCAGCCTGACGCGCTACATCAc                                                                                                            >  2:415682/1‑251 (MQ=255)                                                                                                                                        gctgaggAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTTGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGAACAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCTCTACATCACg                                                                                                           <  2:288585/244‑1 (MQ=255)                                                                                                                                           gctgAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGaa                                                                                                         <  1:453014/249‑1 (MQ=255)                                                                                                                                            ctgAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATgg                                                                                                      >  1:420694/1‑251 (MQ=255)                                                                                                                                              gAGCGACCAGGTCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTTCCCAGCGTGCTGTTATTTAGCACCCGGGCAACCGTTGCCACAGAGACGCCAGCCTGACGCCCTACATCACGAATGGTg                                                                                                    >  2:121008/1‑251 (MQ=255)                                                                                                                                               aGCGACCAGATCCCCCGCTTTTACCAGCGAGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGCGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGAAGCGCTCCATCCCGAATGGTg                                                                                                    >  2:308547/1‑250 (MQ=255)                                                                                                                                               aGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCATAAACGTCCATCACCACCACGCCAATGGGGGCGCTAGCCTGAGTTGCCAGCGCCTGGGCTTTg                                                                                                                                                                                                                                                 <  2:60535/109‑1 (MQ=255)                                                                                                                                               aGCGACCAGATCCACCGCTTTTACCAGCGCGACGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTg                                                                                                                                                                                                                                                 >  1:60535/1‑109 (MQ=255)                                                                                                                                                gCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGAt                                                                                                  <  1:375718/251‑1 (MQ=255)                                                                                                                                                 cGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTACCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGGGCTGTTCATAAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGAACTACATCACgtcgtgtgatc                                                                                                 >  2:280761/1‑241 (MQ=255)                                                                                                                                                    ccAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCat                                                                                               >  1:341552/1‑250 (MQ=255)                                                                                                                                                        aTCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTc                                                                                           >  1:11778/1‑250 (MQ=255)                                                                                                                                                                 ttttACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGATTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCCCGAATGGAGCTCATATTCACTACCTGtt                                                                                 >  2:207756/1‑251 (MQ=255)                                                                                                                                                                  tttACCAGCGCGCCGAAAACCGCATCAGAAACGTCCATCCCCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGt                                                                                  <  2:406458/249‑1 (MQ=255)                                                                                                                                                                         gcgcgcCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTGGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGAtt                                                                         <  2:520383/251‑1 (MQ=255)                                                                                                                                                                             gcCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGAGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAg                                                                     <  1:10587/251‑1 (MQ=255)                                                                                                                                                                                gAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTa                                                                   >  2:388561/1‑250 (MQ=255)                                                                                                                                                                                              aaaCGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTg                                                    <  1:198074/251‑1 (MQ=255)                                                                                                                                                                                                        caccaccacGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCGGCTAttt                                          <  1:200643/251‑1 (MQ=255)                                                                                                                                                                                                         cccaccacGCCAATGTTGTCGCTATCCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCGCACTCACGGCGTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTTACGCGCTACATCACGAATTGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCGGCTAtttt                                         <  2:494366/250‑1 (MQ=255)                                                                                                                                                                                                             ccacGCCACTGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCGGCTATTTTGTc                                      >  2:363233/1‑250 (MQ=255)                                                                                                                                                                                                                  ccctaggTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCGGCTATTTTg                                        >  2:263562/6‑243 (MQ=255)                                                                                                                                                                                                                  ccAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCGGCTATTTTg                                        <  1:263562/243‑1 (MQ=255)                                                                                                                                                                                                                        gttccGCTTACCTGAGGTGCCAGCGCCTGGGCTTTGGCGTTTGGTCTATAATCCAGCTCACTTACGGCTTTCATTACTGCTTCACGCTTGTCGGCACTGACCAGCGTGCTGTTATTGAGCATCCGGGAATCCGGTGCCACAGAGACGCCATCCTGACGCGCTTCTTCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCTGCTATTTTGTCCGCACTAAATAc                          <  2:310695/247‑1 (MQ=255)                                                                                                                                                                                                                                     gAGTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCGGCTATTTTGTCAGCACTGAATACAGGACTTCGTg               <  1:406196/249‑1 (MQ=255)                                                                                                                                                                                                                                       gTTGCCAGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCGGCTATTTTGTCAGCACTGAATACAGGACTTCGTGAATc           <  1:388561/251‑1 (MQ=255)                                                                                                                                                                                                                                             aGCGCCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCGGCTATTTTGTCAGCACTGAATACAGGACTTCGTGAATCGAGTca     <  2:69367/251‑1 (MQ=255)                                                                                                                                                                                                                                                gcCTGGGCTTTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCGGCTATTTTGTCAGCACTGAATACCGGACTTCGTGCATCGAGTcaccg  >  2:374980/1‑249 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATT‑AACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACGGCTTTCATTACTGCTTCACGCGTGTCGGCACTGACCAGCGTGCTGTTATTGAGCACCCGGGAAACCGTTGCCACAGAGACGCCAGCCTGACGCGCTACATCACGAATGGTGATCATATTCACTACCTGTTATGAGATTGCAGTAAATGACTGCTTGCTGGCGGCTATTTTGTCAGCACTGAATACAGGACTTCGTGAATCGAGTCACAG  >  NC_000913/2241273‑2241761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑