Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,987,499 C→A intergenic (+71/+8) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,987,4990CA94.1% 48.0 / NA 17intergenic (+71/+8)ftnB/yecJputative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (8/8);  total (9/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

ATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAACCAC  >  NC_000913/1987265‑1987716
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                         
atTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCAGTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGTTTTTTTTAATGGGa                                                                                                                                                                                                           <  2:230390/251‑1 (MQ=255)
                   gcgcGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATGGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTGGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAAc                                                                                                                                                                                        <  1:259840/251‑1 (MQ=255)
                    cgcgATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGACCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACt                                                                                                                                                                                       >  1:505608/1‑251 (MQ=255)
                                aaaGAACAGCAGCATGATGGTCTGTTGCTGCAGACCAGTCGTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGCCCGACCAACATGTTCTGAAGGTCGTGTTTATCCAGCTGCATTGAGCGTAATCGGCGCTAATTCATTGCGTTGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGTAGCAGAGGGTTGGTTAGTGGTATTCACCCTGTGGGGTAAACTTGAGTTCAATaa                                                                                                                                                                           <  1:249156/251‑1 (MQ=255)
                                   gAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAgcg                                                                                                                                                                        >  2:126446/1‑251 (MQ=255)
                                     aCAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGcgcg                                                                                                                                                                      <  1:54653/251‑1 (MQ=255)
                                                                aaCCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTg                                                                                                                                           >  2:141360/1‑251 (MQ=255)
                                                                  ccATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGGGCACTGTGCAGACAGGCCAACATGTTCTGAATGTCGTGTCACACCCACTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGGTTTGAGAAACCGCGGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGAc                                                                                                                                         >  1:24129/1‑251 (MQ=255)
                                                                                   cgcAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGcggcggcgg                                                                                                                        >  2:492549/1‑251 (MQ=255)
                                                                                          cGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTGTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGAGGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGtt                                                                                                                 <  1:80805/251‑1 (MQ=255)
                                                                                                                        cctaccAATATGTTCTGAATGTTGTGTCACACCAGTTGCATTGATCATCAGCGTCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCTCCCTGTTTGGTAAGCTTGAGTTCAATAAGCTCGGGGGCTTTTTGGATTGCCCGCATGGTGACTGTTTCTGCGGCGGCGGGATGGTTAGTAAAGGCGATATTCTTCATCTGTCTGGa                                                                                   <  2:505608/248‑1 (MQ=255)
                                                                                                                                 aTGTTCTGAATGTCGTGTCACACAAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCTGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTc                                                                          >  2:303786/1‑251 (MQ=255)
                                                                                                                                                       ccAGCTGCATTGATCATCATCGGCGATAATGCATTGCGACGAGGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTGTTTTAATGGGATTCACACTGTGGGGTAAACGTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTGTGCGGCGGCGGGATGGTTAGTAAAGTCGATAGGCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGTGCGAta                                                    <  2:140166/251‑2 (MQ=255)
                                                                                                                                                                    tcatcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACAt                                       >  1:122745/1‑251 (MQ=255)
                                                                                                                                                                                       aTTGCGCCGATGAAGGTTTTGAGAATCCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGGGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTa                                                                                                                <  1:38624/159‑1 (MQ=255)
                                                                                                                                                                                                  gAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACCTTTTTTCAAGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGgca           >  2:42392/1‑249 (MQ=255)
                                                                                                                                                                                                          tGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGCCATTTTTTCACGAACTTCAACGGGCGCGATTACTTCGGGAACATCCAGAATTTGTTTGTTAACCAACTGCCACGCACCcac  >  2:222309/1‑250 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                         
ATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAACCAC  >  NC_000913/1987265‑1987716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑