Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,859	T→C	Y110C (TAT→TGT)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,859	0	T	C	100.0%	78.7 / NA	24	Y110C (TAT→TGT)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/15);  total (9/15)

AGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACC  >  NC_000913/1358614‑1359097                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               aGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCAGGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTGAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATcc                                                                                                                                                                                                                                           <  2:206943/251‑1 (MQ=255)  gACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAATATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCca                                                                                                                                                                                                                                          <  2:292660/251‑1 (MQ=255)        gCCAACGCCTTCTCCGTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCg                                                                                                                                                                                                                                    <  1:471974/251‑1 (MQ=255)               ccTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCGTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCa                                                                                                                                                                                                                             <  1:420305/251‑1 (MQ=255)                              aGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATcc                                                                                                                                                                                                              <  2:218313/251‑1 (MQ=255)                                 cccggAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTGCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAATAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGAc                                                                                                                                                                                                           <  2:508017/247‑1 (MQ=255)                                    ggAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTg                                                                                                                                                                                                        <  2:115415/250‑1 (MQ=255)                                                      aaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGAAGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGGAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGGTAATCGACTTTTg                                                                                                                                                                                      >  1:445413/1‑251 (MQ=255)                                                                 gtgggAGATTTGCACCAGTTCAAACCGTGTAGTGAAGTTAGCTACCAGGTGGACGCACTTCAGATTCGCGGCGGTTAAAATGGCTACGAAGGTTACCACCCACTCCCTCGGCGGCTCTTCCGGGAAGAGGGCGTAGAGATAGATTTTCGCCAACAAGACTTTAATCAGCGGCAAAAATAGACAATCCATCAGCGATTACCAGCCGCCCATAAATCCGACGTGCGGGTTAATCGACTTTTGCTCGTAGGtat                                                                                                                                                                           <  2:520901/248‑1 (MQ=255)                                                                            gCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCg                                                                                                                                                                                                             <  2:163854/206‑1 (MQ=255)                                                                            gCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTTGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCg                                                                                                                                                                                                             >  1:163854/1‑206 (MQ=255)                                                                                cAGTACAAACCGGGTATCGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGCCGAAGGTTAACACCCACACCCACGGCGGCCCTTCCGGGAAGAGGGCGGAGAGAAAAATTTTCGGCCACAAGAACATAATAATCCGCAAAAAAAGACAATCCAGAAGCGAAGACCAGCCGACCATAAAACCGACGTGCGGGGTAAACGACATTTTAGCGTAGGGATAGGCCGAAcacgcat                                                                                                                                                            >  2:157497/1‑245 (MQ=255)                                                                                    aCAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCgg                                                                                                                                                        <  1:20059/251‑1 (MQ=255)                                                                                                  gAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCa                                                                                                                                          <  1:240569/251‑1 (MQ=255)                                                                                                                                    gcggcgGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACAcc                                                                                                        <  1:444100/251‑1 (MQ=255)                                                                                                                                      ggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACAcccc                                                                                                      >  1:270977/1‑250 (MQ=255)                                                                                                                                                                                       cccGGGAAGAGGGCGAAGAGATAGATTTCCGCCAACAGGACGTTAATCATCGGCAAAAAGAGACAACCAAGCAGCGATGCCCAGCCGACCAAAAACCCGTCGGGCGGGTTAATCGACTTTGGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACGGGCGAACCAGTTTGCCTTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGga                                                     <  1:130414/250‑1 (MQ=255)                                                                                                                                                                                         cGGGAAGAGGGCGGCGAGATAGATTTTCGCCACCAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCCGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGACAGCGCCAGCAAATAGGCCGCCGGAACGTGACCGTCGCTAATGCCgg                                                      >  2:509654/1‑248 (MQ=255)                                                                                                                                                                                                agGGCGGAGAGATAGATTTCCCCAAACAAGACGTTAATCACCGGAAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAACCCGACGTGCGGTTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGACCCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATAcc                                            <  1:23328/251‑1 (MQ=255)                                                                                                                                                                                                          aatagatTTTCTCCAAAAAGAGTTTAAGCATCGGAAAAAAGAGACAACCCAGCGGCGATGACGAGCCGACTAAAAACCCGACGTGCGGGTTAATCGCCTTTGGGGCTTATGTATAGGCCGACCCGGCCTCAGGAAACTGGCGAACCAGTTTGCCGTAGCTGGTAGCGTTAAGCAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCa                                  <  2:360381/250‑1 (MQ=255)                                                                                                                                                                                                                 tttCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACAAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACgg                           >  1:344889/1‑251 (MQ=255)                                                                                                                                                                                                                              aCGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGCTGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGAATCAAATACGGTCATCGGCGTg                >  1:226539/1‑249 (MQ=255)                                                                                                                                                                                                                                          ggCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGCAATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTACACAGCACACCCGCCAGCGCCAGCCAATAGGACGCCGGAACGTGACAGCCGCTAATGCCAGAGACAATACCAAAAGCATCAAATACGGTCATCGGCGTGAGACAGGCCACAcc  >  2:8810/1‑251 (MQ=255)                                                                                                                                                                                                                                            cAAAAAGAGACAATCCAGCAGCGATGACCAGCCGCCCATAAATCCGACGTGCGGGTTCATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGCACCCGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagc                                                                                                 >  1:249357/1‑154 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               AGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACC  >  NC_000913/1358614‑1359097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑