Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,298,624	C→T	P9P (CCC→CCT)	ychE →	MarC family putative inner membrane protein YchE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,298,624	0	C	T	100.0%	68.9 / NA	21	P9P (CCC→CCT)	ychE	MarC family putative inner membrane protein YchE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/12);  total (9/12)

CCATTATAGCTAACAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCAATTGATTCGTTCCGTATCGCCGGGGGTATCCTG  >  NC_000913/1298397‑1298855                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        ccATTATAGCTAACAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCgg                                                                                                                                                                                                                  <  1:86910/251‑1 (MQ=255)                    ataaATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAAc                                                                                                                                                                                               <  1:514089/251‑1 (MQ=255)                    ataaATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGCTTACATCAAATTTTTCATCGGGTTATTTGCGCTGGTCAAc                                                                                                                                                                                               <  2:6331/251‑1 (MQ=255)                                                        gTTTTGCACAACTAGATAACAATAACGAATGATATCAATTGTAAGTAGTTAGGATGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCGTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATGTGCGCTGTGCAATCCGGTAGCGGTTATTCCCTGCTTTATCAGCAGGAc                                                                                                                                                            <  2:535448/250‑1 (MQ=255)                                                                      gATAACAATAACGAATGATAGCAATTTAAAGTAGTTAGGAGGTGAAAAATGATGTCAAAAGGCGAATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTCCTTCAAATTTTTCATCGGGCTATTCGAGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGCCCAGTTATCAGACAGc                                                                                                                                             >  2:395001/1‑251 (MQ=255)                                                                         aaCAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGc                                                                                                                                          >  1:108740/1‑251 (MQ=255)                                                                                            aaTTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAAc                                                                                                                       <  1:537301/251‑1 (MQ=255)                                                                                                             aggTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACctgtc                                                                                                      >  2:13293/1‑251 (MQ=255)                                                                                                                    aaaTGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGcc                                                                                               <  1:114971/251‑1 (MQ=255)                                                                                                                           gTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCt                                                                                        >  1:414142/1‑251 (MQ=255)                                                                                                                             cAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTtg                                                                                      >  1:383988/1‑251 (MQ=255)                                                                                                                                     gTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGTAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGAtctc                                                                              <  1:175813/251‑1 (MQ=255)                                                                                                                                                  gTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCCAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGc                                                                 >  1:520941/1‑251 (MQ=255)                                                                                                                                                                atttatGGCTAACATTATCCGGCTTTTGCTTCGAAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTAc                                                    >  2:260609/1‑250 (MQ=255)                                                                                                                                                                   tatGGCTAACATGATCCGGCTTTTGCGTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCAGCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCGGGGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAAc                                                 <  2:345905/250‑1 (MQ=255)                                                                                                                                                                                      cTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCAATTGAt                             >  2:78282/1‑251 (MQ=255)                                                                                                                                                                                           tcTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCAATTGATTCGt                         <  2:520941/249‑1 (MQ=255)                                                                                                                                                                                             ttCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCAATTGATTCGTTcc                      >  1:400332/1‑251 (MQ=255)                                                                                                                                                                                                   gCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCAATTGATTCGTTCCGTATCg                <  2:128606/251‑1 (MQ=255)                                                                                                                                                                                                   gCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCAATTGATTCGTTCCGTATCg                <  1:78282/251‑1 (MQ=255)                                                                                                                                                                                                                 aGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCAATTGATTCGTTCCGTATCGCCGGGGGTATCCtg  <  1:13293/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        CCATTATAGCTAACAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCAATTGATTCGTTCCGTATCGCCGGGGGTATCCTG  >  NC_000913/1298397‑1298855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑