Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,779	C→T	intergenic (‑157/+3)	tyrT ← / ← purU	tRNA‑Tyr/formyltetrahydrofolate deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,779	0	C	T	100.0%	87.7 / NA	25	intergenic (‑157/+3)	tyrT/purU	tRNA‑Tyr/formyltetrahydrofolate deformylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/10);  total (15/10)

GCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCAC  >  NC_000913/1287594‑1288002                                                                                                                                                                                          |                                                                                                                                                                                                                                gCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGc                                                                                                                                                                >  1:186781/1‑251 (MQ=255)         tGGCCGCTCGGGAACCCCACAACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGAGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGCAAAATGAACTGGTTGAGTAATTTTCCTCAGTAACGGATTAAAGGAAACCAGTTATTTTTGCTGGTGATTAAAGAACAATCGTTCGATTACCGTACACAAAGACGCGCTGCGCCAGTACTTTGTATATTGCACGACtt                                                                                                                                                         >  2:440709/1‑250 (MQ=255)                             caaggggtaatgaagtttaaaggCCTGATCCCTTATCGGGCAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTc                                                                                                                                     >  1:180030/22‑250 (MQ=255)                                                                         ggggCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGGTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGGATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGAc                                                                                        <  2:186781/251‑1 (MQ=255)                                                                               catcatATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGTCATGAAt                                                                                  <  1:103008/251‑1 (MQ=255)                                                                                  catATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAAc                                                                               >  1:497395/1‑251 (MQ=255)                                                                                                 gccgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATgatgat                                                                >  2:209656/1‑251 (MQ=255)                                                                                                      tGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGc                                                           >  1:55813/1‑251 (MQ=255)                                                                                                         aaaGTGTTACGTTGCGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCtt                                                        >  1:407471/1‑251 (MQ=255)                                                                                                                           aaaTGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGCTTAAAGAATAATCGTTAGCTTACCGTAAACAAAGACGCGCTGTGCCAGTACt                                                                                                                                                                           >  2:363859/1‑118 (MQ=255)                                                                                                                           aaaTGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACt                                                                                                                                                                           <  1:363859/118‑1 (MQ=255)                                                                                                                             aTGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTg                                                              >  1:132108/1‑225 (MQ=255)                                                                                                                             aTGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTg                                                              <  2:132108/225‑1 (MQ=255)                                                                                                                                    ggTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTg                               >  1:139536/1‑249 (MQ=255)                                                                                                                                    ggTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAg                             <  1:23784/251‑1 (MQ=255)                                                                                                                                                ttCATCTGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCa                  >  1:144116/1‑250 (MQ=255)                                                                                                                                                                tAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACg                                                                                                                                                             <  1:28304/95‑1 (MQ=255)                                                                                                                                                                tAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACg                                                                                                                                                             >  2:28304/1‑95 (MQ=255)                                                                                                                                                                tAAAGGTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCAcaccac  <  2:180030/250‑1 (MQ=255)                                                                                                                                                                     gTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTg                                                                         >  1:476245/1‑174 (MQ=255)                                                                                                                                                                     gTAACCAGTTATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTg                                                                         <  2:476245/174‑1 (MQ=255)                                                                                                                                                                              tATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATc            >  2:343821/1‑226 (MQ=255)                                                                                                                                                                              tATTTTTGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCAGGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATc            <  1:343821/226‑1 (MQ=255)                                                                                                                                                                                    tGCTGGTGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGAATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTca                                      >  1:521735/1‑194 (MQ=255)                                                                                                                                                                                    tGCTGGTGATTAAAGAATAAGCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCGCGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGTATCATATCTTCAGCTGTGTAGGTATTAGCGACATGAATAACGTCCTGCAGGATGATCGGTCCTTCGTCCTGATTGTCATTca                                      <  2:521735/194‑1 (MQ=255)                                                                                                                                                                                          |                                                                                                                                                                                                                                GCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCAC  >  NC_000913/1287594‑1288002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑