Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	96,855	G→A	R285H (CGT→CAT)	mraY →	phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	96,855	0	G	A	100.0%	220.3 / NA	61	R285H (CGT→CAT)	mraY	phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/27);  total (34/27)

ATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACGTCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGATTATTTCGCTGATGCTGGTTCTGATTGGTCTGGCAACGCTGAAGGTACGTTAATCATGGCTGATTATCA  >  NC_000913/96608‑97100                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aTGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCa                                                                                                                                                                                                                                                     <  1:362818/251‑1 (MQ=255)    ccgcccgcatctgccGCCCGTGGTTTCGCGCTGCCGGCGTGCGCGACCGGCAATCTGCACCTTGCCAGCTCCTTCCCTCTACCGTATCTGCGACCCGCCGGGGACCCGGTTATTCTCTGTACCGCGCTCGTCGGGGCAGGACTGCGCTTCCTGTGGTTTAACACCTATCCCGCCCAGGCCTTTATGGGCGATGTCGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCCTCATGCTACATCAgg                                                                                                                                                                                                                                                   >  2:169636/15‑250 (MQ=255)         cGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTcc                                                                                                                                                                                                                                             <  2:225814/251‑1 (MQ=255)            aTTTGTCGCCGGTGGTTTTGCGCGGGTGGCGTGGGCGACCGGCAATAGGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTCTGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCctgc                                                                                                                                                                                                                                          <  2:133922/251‑1 (MQ=255)               tGTCGCCTGTGGTTTTTCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCctgctg                                                                                                                                                                                                                                        <  2:380695/250‑1 (MQ=255)                gTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCACTATGAACTTTGACAGCTACTTGCATATACCGTATCTGCGTCACGCCGGGGAACTGGTTATTGTCTGAACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTggg                                                                                                                                                                                                                                      >  1:298976/1‑250 (MQ=255)                  cGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTTCTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGa                                                                                                                                                                                                                                    <  2:193415/251‑1 (MQ=255)                   gCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCTTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGAt                                                                                                                                                                                                                                   <  2:66351/251‑1 (MQ=255)                     cGGTGGTTGTGCGCTGGGGACGGGGGCGACCGACACTATGCACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGCCTTCCTGTGGTTTACCACCTATCCGGCGCAGGTCTTTATTGGAGATGTAGGTTAGCTGTCGTTTGGAGGGGCTTGAGGCATTATCGCCGTAATGCTACATCAGGAATTCCTGCTGGTGAtt                                                                                                                                                                                                                                  >  2:381124/1‑250 (MQ=255)                           ttttGCGTTGGTGGCGTGGGCGCCCGGCAATATGAACTTTGCCAGCTTCTTGCATATACCGGATCTGCGACGCGCCGGGGAACTGGTGATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTGCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGTGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATgggg                                                                                                                                                                                                                            <  2:196409/250‑1 (MQ=255)                             ttGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCTTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTTGTGATTATgggggg                                                                                                                                                                                                                          <  1:470359/250‑1 (MQ=255)                                          gTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGt                                                                                                                                                                                                             >  1:370887/1‑249 (MQ=255)                                          gTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGt                                                                                                                                                                                                             >  1:450932/1‑249 (MQ=255)                                                                   gCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGCCTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGCGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGTGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAggg                                                                                                                                                                                  >  2:252999/1‑250 (MQ=255)                                                                               cATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTTGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTaa                                                                                                                                                                      <  2:350224/251‑1 (MQ=255)                                                                                       gTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTCTCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGcgcg                                                                                                                                                              >  2:46550/1‑251 (MQ=255)                                                                                         aggtgggACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTAGCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGa                                                                                                                                                            <  2:146899/245‑1 (MQ=255)                                                                                               gACACGCCGGGTAACTGGTTATTGTCTGTGCCGCGATTGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGTCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGTTCGGCTCCTTTAAACTGCGCGGACAACGt                                                                                                                                                      <  2:530237/251‑1 (MQ=255)                                                                                                 cactccgtggatctggttatctgcTGTACCTCGATAGTCGGGGCAGGACTGGGCGTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTGTATGGGCGATGTAGGGTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTTCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCTGACAACGTAt                                                                                                                                                    <  2:196335/228‑1 (MQ=255)                                                                                                       ggggAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGGGGTGCGTGAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCg                                                                                                                                              >  2:155341/1‑251 (MQ=255)                                                                                                          gAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGTTTCGCTGGCGTTAGGTGGTGCGGTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCa                                                                                                                                            <  1:316358/250‑1 (MQ=255)                                                                                                          gAACTGGCTATTGTCTGTACCGCGATAGACGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCAGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTCCATCCGTAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCATGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCgacc                                                                                                                                           >  2:316358/1‑248 (MQ=255)                                                                                                             cTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGGCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGc                                                                                                                                        >  1:11931/1‑251 (MQ=255)                                                                                                               ggTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGTTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGGCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCAc                                                                                                                                      <  1:50458/251‑1 (MQ=255)                                                                                                                            aCCGCGATAGGCGGGGCAGGACTGGGTTTCCTGTGGTTTAACCCCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACAGCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACTCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATcacc                                                                                                                          <  2:450932/250‑1 (MQ=255)                                                                                                                                cgATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTAt                                                                                                                     <  1:116323/251‑1 (MQ=255)                                                                                                                                 gATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTGTTTTCCGCATGGCACCGATTCATCACCACTATg                                                                                                                    >  1:465539/1‑251 (MQ=255)                                                                                                                                    aGTCGGGGCAGGACTGGGCTTCCTGAGGTTTACCACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGATCGCTGGAGCTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATAAGGAATACCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGCAAAGCTTTCTGTCCTCCTGCAGGTCGGCTCATTTACACTGCGCGGACAACGTATTTTCCGCATGTCACCGATTCATCACCACTATGAAc                                                                                                                 >  1:305152/1‑251 (MQ=255)                                                                                                                                     gTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCGATCCGGCGCAGGTGTTTATGGGCGATGTAGGTTCGCTGGCGTTATGTGTTGCGTTAGGCATTAGCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGAGGATGGGGGGCG‑GTGTCGTGGTAGATACGCTTTGTGGCATCCTGCAGGTCGGCTCCTGTAATCTGCGCGGACAACGGATTTTCCGCATGGCACCGATTCATCACCACTATGAACt                                                                                                                <  1:518746/251‑1 (MQ=255)                                                                                                                                       cGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTCGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAAc                                                                                                                                                                    >  2:331981/1‑197 (MQ=255)                                                                                                                                       cGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGTGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAAc                                                                                                                                                                    <  1:331981/197‑1 (MQ=255)                                                                                                                                            cAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGc                                                                                                         >  2:135084/1‑251 (MQ=255)                                                                                                                                               gACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGACTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTgg                                                                                                      >  1:536009/1‑251 (MQ=255)                                                                                                                                                  tGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCg                                                                                                   >  1:84273/1‑251 (MQ=255)                                                                                                                                                   gggCTTCCTGTGGTTTAACACCTCTCCGGCGCAGGTCTTTATGGGCGATGTAGGTTAGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTTTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGAATTTTCCGAATGGCACAGATTAATCACCACTATGAACTAAAAGGCTGGCCgg                                                                                                  >  2:327408/1‑251 (MQ=255)                                                                                                                                                   gggCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCAGCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGGATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGcc                                                                                                    <  1:77981/249‑1 (MQ=255)                                                                                                                                                               gTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCa                                                                                      <  2:47406/251‑1 (MQ=255)                                                                                                                                                                   aaCACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATtg                                                                                   <  1:265264/250‑1 (MQ=255)                                                                                                                                                                    acacCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATtgtg                                                                                 <  1:469661/251‑1 (MQ=255)                                                                                                                                                                      cccTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCg                                                                               <  1:376158/250‑1 (MQ=255)                                                                                                                                                                             cGGCGCAGGCCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTgg                                                                        >  2:538390/1‑251 (MQ=255)                                                                                                                                                                              ggCGCAGATCTTCATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGATAGGCATTATCGCCGTACTGCTCCATCAGGAATTACTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTgg                                                                        >  2:299457/1‑250 (MQ=255)                                                                                                                                                                                cgcAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGatt                                                                     >  1:311753/1‑251 (MQ=255)                                                                                                                                                                                   aGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTg                                                                         >  2:164754/1‑244 (MQ=255)                                                                                                                                                                                   aGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTg                                                                         <  1:164754/244‑1 (MQ=255)                                                                                                                                                                                    ggTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGATTAttt                                                                 >  1:447220/1‑251 (MQ=255)                                                                                                                                                                                        tttATGGGCGATGTAGGTTCGCTGGAGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGAAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTAGGCTCCTTTAAACTGCGCGGACAAAGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGAGAGTTTCTGGATTATTTCGCt                                                             >  2:446886/1‑251 (MQ=255)                                                                                                                                                                                          tATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGATTATTTCGCTg                                                            >  1:279751/1‑250 (MQ=255)                                                                                                                                                                                            tGGGCGATGTAGGTTCGCTGGCGTTAGGTGGAGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGATTATTTCGCTGATg                                                         >  1:33333/1‑251 (MQ=255)                                                                                                                                                                                                      aGGTGCGCTGGGGTTAGGTGGGGCGTGAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGTGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCTCATGGCACCGATGCATCACCACTTTGAACTTAAAGTCTGGCCGGAACTGCGCGTCATTGTGCGTTTCTGGATTATTTCGCTGTTGCTGGTTCta                                                <  2:130126/250‑2 (MQ=255)                                                                                                                                                                                                           cGCTGGCGTTAGGGGGTGCGTTAGGCATTATCGCCGTACTGATCAATATGGAATTCCTGCTGGTGATTATGAGGGGCGTGA‑TCGTGGTAGACACGCTTTCTGACATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGCCAACGTATTTTCCGCATGGCACAGATTCATCCCCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGATTATTTCGCTGATGCTGGTTCTGCTTgtt                                          >  2:532467/1‑249 (MQ=255)                                                                                                                                                                                                              tGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAg                                                                                                           >  1:190227/1‑183 (MQ=255)                                                                                                                                                                                                              tGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACcgc                                                                                            >  1:179393/1‑198 (MQ=255)                                                                                                                                                                                                              tGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACcgc                                                                                            <  2:179393/198‑1 (MQ=255)                                                                                                                                                                                                                      ggtggtGCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGATTATTTCGCTGATGCTGGTTCTGATTGGTCTGGCAACGCt                               >  2:405829/1‑251 (MQ=255)                                                                                                                                                                                                                           tgCGTTAGGCATTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGATTATTTCGCTGATGCTGGTTCTGATTGGTCTGGCAACGCTGAAgg                          >  1:411361/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGATTATTTCGCTGATGCTGGTTCTGATTGGTCTGGCAACGCTGAAGGTACGTTAATc                >  1:25782/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTTATCGCCGTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGATTATTTCGCTGATGCTGGTTCTGATTGGTCTGGCAACGCTGAAGGTACGTTAATc                >  1:367380/1‑251 (MQ=255)                                                                                                                                                                                                                                              gTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCCTTGTGCGTTTCTGGATTATTTCGCTGATGCTGGTTCTGATTGGTCTGGCAACGCTGAAGGTACGTTAATCATGGcgggt       >  2:395047/1‑247 (MQ=255)                                                                                                                                                                                                                                              gTACTGCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACACTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCTTGGCCCCGATTCATCACCACTATGAACTGAACGGCTGGCCGGACACGCGCGTACTTGTGCGTTTCTGGCTTATTTCGCTGATGCTGGTTATGCTTGGTCTGGCAACGCTGAAGGTACGTTCATCAGTGCTGAt       >  2:463103/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gCTACATCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGATTATTTCGCTGATGCTGGTTCTGATTGGTCTGGCAACGCTGAAGGTACGTTAATCATGGCTGATTATCa  <  1:135084/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       ATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGTCTTTATGGGCGATGTAGGTTCGCTGGCGTTAGGTGGTGCGTTAGGCATTATCGCCGTACTGCTACGTCAGGAATTCCTGCTGGTGATTATGGGGGGCGTGT‑TCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGGATTATTTCGCTGATGCTGGTTCTGATTGGTCTGGCAACGCTGAAGGTACGTTAATCATGGCTGATTATCA  >  NC_000913/96608‑97100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑